Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,807	(T)5→4	coding (775/2448 nt)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,803	0	T	.	100.0%	239.2 / NA	57	coding (779/2448 nt)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/29);  total (28/29)

TTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAA  >  NC_000913/3565665‑3565937                                                                                                                                           |                                                                                                                                        ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGtt                                                                                                                                        <  1:812541/140‑1 (MQ=255)  tATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGttt                                                                                                                                       >  2:510279/1‑140 (MQ=255)  tATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGttt                                                                                                                                       >  2:905377/1‑140 (MQ=255)  tATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGttt                                                                                                                                       >  1:30418/1‑140 (MQ=255)      cAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGttttatc                                                                                                                                   >  2:396003/1‑137 (MQ=255)                tGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGcc                                                                                                                        <  1:396003/140‑1 (MQ=255)                 ggcggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcg                                                                                                                       >  1:861535/1‑140 (MQ=255)                  gcggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcgc                                                                                                                      <  2:1122127/140‑1 (MQ=255)                   cggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcgcg                                                                                                                     <  1:1013163/140‑1 (MQ=255)                   cggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcgcg                                                                                                                     <  2:812354/140‑1 (MQ=255)                   cggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcgcg                                                                                                                     >  1:812354/1‑140 (MQ=255)                   cggcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCcgcg                                                                                                                     <  2:816662/140‑1 (MQ=255)                     gcTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGaa                                                                                                                   >  1:1069344/1‑140 (MQ=255)                      cTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAag                                                                                                                  >  2:31863/1‑140 (MQ=255)                      cTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAag                                                                                                                  >  1:683029/1‑140 (MQ=255)                      cTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAag                                                                                                                  >  2:304386/1‑140 (MQ=255)                         aaaaTGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAagtag                                                                                                               <  2:861535/140‑1 (MQ=255)                                ccTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCt                                                                                                        >  1:1199171/1‑140 (MQ=255)                                   gAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGt                                                                                                     <  2:492380/140‑1 (MQ=255)                                         tCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑tt                                                                                              >  1:111243/1‑140 (MQ=255)                                             ggAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTAcc                                                                                          <  1:1079577/140‑1 (MQ=255)                                                 aCCTGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAgg                                                                                      >  1:70175/1‑140 (MQ=255)                                                   cAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCAACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGtt                                                                                    >  1:454047/1‑140 (MQ=255)                                                       aaGTATTCCTGACGCAGGCGCAGCTCACGCCCAGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAAtt                                                                                <  2:867113/140‑1 (MQ=255)                                                        aGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAAttt                                                                               <  1:56917/140‑1 (MQ=255)                                                            ttCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCt                                                                           >  1:154115/1‑140 (MQ=255)                                                                  aCGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTTACCGAGGTTAATTTCGCTACTgg                                                                      <  1:342400/140‑1 (MQ=255)                                                                      aGGCGCAGCTCACGCCCGGAGTAGGTGGAGTAATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTgc                                                                 >  2:1187546/1‑140 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAAttt                                                                               <  2:949124/125‑1 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAAttt                                                                               >  1:949124/1‑125 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTgcg                                                                >  2:689210/1‑140 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTgcg                                                                <  1:776689/140‑1 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTgcg                                                                <  2:473213/140‑1 (MQ=255)                                                                       ggCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTgcg                                                                <  1:689210/140‑1 (MQ=255)                                                                         cgcAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCa                                                              <  1:565754/140‑1 (MQ=255)                                                                              cTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCca                                                         >  1:289659/1‑140 (MQ=255)                                                                                     ccGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGc                                                  <  2:111243/140‑1 (MQ=255)                                                                                      cGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGc                                                  <  2:1093725/139‑1 (MQ=255)                                                                                      cGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGc                                                  >  1:1093725/1‑139 (MQ=255)                                                                                                ggAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGt                                       <  2:289659/140‑1 (MQ=255)                                                                                                   gTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTcgc                                    >  2:1207478/1‑140 (MQ=255)                                                                                                   gTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTcgc                                    >  1:701247/1‑140 (MQ=255)                                                                                                    tCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGGcgcg                                   >  1:913693/1‑140 (MQ=255)                                                                                                                 gTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGATGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAc                      <  2:1069344/140‑1 (MQ=255)                                                                                                                 gTACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAc                      <  2:701247/140‑1 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGt                                  <  2:957296/127‑1 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGt                                  >  1:957296/1‑127 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTc                           <  2:1122101/134‑1 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTc                           >  1:1122101/1‑134 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                     >  2:773278/1‑140 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                     >  1:751/1‑140 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                     >  1:636314/1‑140 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAc                      >  2:596329/1‑139 (MQ=255)                                                                                                                  tACGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAc                      <  1:596329/139‑1 (MQ=255)                                                                                                                   aCGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCa                    <  2:154115/140‑1 (MQ=255)                                                                                                                   aCGCGAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCa                    <  2:751/140‑1 (MQ=255)                                                                                                                     gcgAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAgg                  <  2:328782/140‑1 (MQ=255)                                                                                                                      cgAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                     <  1:89615/136‑1 (MQ=255)                                                                                                                      cgAGATACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAAcc                     >  2:89615/1‑136 (MQ=255)                                                                                                                          aTACGTTCTCGGAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTa             >  1:1158543/1‑140 (MQ=255)                                                                                                                                    ggAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTa   >  1:1000185/1‑140 (MQ=255)                                                                                                                                     gAGTGG‑TTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTaa  <  2:70175/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                        TTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAA‑TTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAA  >  NC_000913/3565665‑3565937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑