Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,601,884	Δ1 bp	coding (214/474 nt)	yjjB ←	putative succinate exporter YjjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,601,884	0	T	.	100.0%	251.1 / NA	60	coding (214/474 nt)	yjjB	putative succinate exporter YjjB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/36);  total (24/36)

ACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAAC  >  NC_000913/4601750‑4602017                                                                                                                                       |                                                                                                                                      aCCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGAc                                                                                                                                 <  2:8435/140‑1 (MQ=255) aCCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGAc                                                                                                                                 >  1:1137708/1‑140 (MQ=255)  ccTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGg‑accgacc                                                                                                                                >  1:298146/1‑140 (MQ=255)      aCTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCa                                                                                                                            >  2:177223/1‑140 (MQ=255)         ggCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAg                                                                                                                         >  1:112515/1‑140 (MQ=255)         ggCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAg                                                                                                                         >  2:709689/1‑140 (MQ=255)          gCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGa                                                                                                                        >  1:100336/1‑140 (MQ=255)          gCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGa                                                                                                                        <  2:5028/140‑1 (MQ=255)                ttttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCAt                                                                                                                  >  2:194890/1‑140 (MQ=255)                  ttACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATaa                                                                                                                <  1:1165230/140‑1 (MQ=255)                       gccgAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGtt                                                                                                           <  1:68506/140‑1 (MQ=255)                          gAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGAc                                                                                                        <  1:920776/140‑1 (MQ=255)                           aaaTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGAcc                                                                                                       <  2:1137708/140‑1 (MQ=255)                           aaaTCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGAcc                                                                                                       <  1:363653/140‑1 (MQ=255)                              tCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACt                                                                                                    <  1:177223/140‑1 (MQ=255)                              tCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACt                                                                                                    <  2:583294/140‑1 (MQ=255)                               cATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTc                                                                                                   <  1:434814/140‑1 (MQ=255)                               cATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTc                                                                                                   <  2:100336/140‑1 (MQ=255)                                       tataAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCAATAAAGGTTGACCACTCAATATTCa                                                                                           <  2:401156/140‑1 (MQ=255)                                           aGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAAccc                                                                                       <  1:186597/140‑1 (MQ=255)                                            gCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATTTTCAACCCg                                                                                      >  1:599400/1‑140 (MQ=255)                                             ccGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGc                                                                                     <  1:709689/140‑1 (MQ=255)                                               gATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTg                                                                                   <  1:611518/140‑1 (MQ=255)                                               gATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTg                                                                                   <  1:11567/140‑1 (MQ=255)                                               gATATGCCCGAGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTg                                                                                   <  1:296294/140‑1 (MQ=255)                                                atatGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTgg                                                                                  <  2:417889/140‑1 (MQ=255)                                                     cccGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGtcatc                                                                             >  2:258706/1‑140 (MQ=255)                                                          gAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAg                                                                          <  2:1212986/138‑1 (MQ=255)                                                          gAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAg                                                                          >  1:1212986/1‑138 (MQ=255)                                                          gAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGAt                                                                        <  1:74905/140‑1 (MQ=255)                                                                aGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCg                                                                  >  1:741131/1‑140 (MQ=255)                                                                aGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCg                                                                  <  2:112515/140‑1 (MQ=255)                                                                 gggATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCgg                                                                 <  1:932387/140‑1 (MQ=255)                                                                    aTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGaa                                                              >  1:810358/1‑140 (MQ=255)                                                                    aTAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGaa                                                              >  2:802328/1‑140 (MQ=255)                                                                     tAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAAc                                                             >  2:78561/1‑140 (MQ=255)                                                                            cgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATg                                                         >  1:347327/1‑137 (MQ=255)                                                                            cgccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATg                                                         <  2:347327/137‑1 (MQ=255)                                                                              ccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTa                                                    <  2:684594/140‑1 (MQ=255)                                                                              ccACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTa                                                    <  2:741131/140‑1 (MQ=255)                                                                                aCGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATc                                                  <  2:789352/140‑1 (MQ=255)                                                                                   gTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAg                                               <  2:1204893/140‑1 (MQ=255)                                                                                     aaaCACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGcc                                             <  1:194890/140‑1 (MQ=255)                                                                                        cacTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAag                                          <  1:148056/140‑1 (MQ=255)                                                                                             tCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAgcg                                     <  1:78561/140‑1 (MQ=255)                                                                                                   gcgcCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAGGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc                               >  2:513230/1‑140 (MQ=255)                                                                                                   gcgcCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACAcc                               >  2:641871/1‑140 (MQ=255)                                                                                                     gcCAGATACCAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAg                             >  1:623004/1‑140 (MQ=255)                                                                                                             ccAGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGc                     <  2:298146/140‑1 (MQ=255)                                                                                                               aGCGCGACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGccc                   <  2:1078840/140‑1 (MQ=255)                                                                                                                 cgcgACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGc                 >  2:621749/1‑140 (MQ=255)                                                                                                                  gcgACCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCa                <  1:353483/140‑1 (MQ=255)                                                                                                                     aCCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg             >  2:285354/1‑140 (MQ=255)                                                                                                                     aCCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg             >  1:226685/1‑140 (MQ=255)                                                                                                                     aCCATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACgg             >  2:16129/1‑140 (MQ=255)                                                                                                                      ccATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACggg            >  2:105289/1‑140 (MQ=255)                                                                                                                       cATTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATTTTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGa           <  2:599400/140‑1 (MQ=255)                                                                                                                        aTTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa          <  2:623004/140‑1 (MQ=255)                                                                                                                        aTTGAATACCAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGaa          <  1:641871/140‑1 (MQ=255)                                                                                                                                ccAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAac  >  1:520761/1‑140 (MQ=255)                                                                                                                                ccAATGG‑ACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAac  >  1:394808/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCTAACTGGCTGATTTTTACCGCCGAAATCATTGCGGTATAAGCCGATATGCCCGGGAACATAGGGATAACGGCCGCCACGGTAAACACTTTCGGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAAC  >  NC_000913/4601750‑4602017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑