Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→7	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,323	0	G	.	96.6%	232.9 / 2.1	59	noncoding (79/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base . (29/28);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-02

CCTCGTTTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTT  >  NC_000913/4606193‑4606462                                                                                                                                       |                                                                                                                                            ccTCGTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gggggggac                                                                                                                                     >  1:629581/1‑138 (MQ=255) catCGTTTGGTGCGGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTCCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gggggggac                                                                                                                                     <  2:846634/138‑3 (MQ=21)     gTTTGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGa                                                                                                                                <  1:680310/140‑1 (MQ=255)      tttGGTGC‑GAGGGGGGGGGACTTGAACCCCCAGGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGaa                                                                                                                               <  1:721912/140‑1 (MQ=21)      tttGGTGC‑GAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGaa                                                                                                                               >  1:530947/1‑140 (MQ=255)      tttGGTGC‑GAGGGGGGGGGACTTGAACACCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gggggg                                                                                                                                        <  2:638153/132‑1 (MQ=255)      tttGGTGC‑GAGGGGGGGGGACTTGAACACCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑gggggg                                                                                                                                        >  1:638153/1‑132 (MQ=255)          gTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAAccccc                                                                                                                          >  2:938177/1‑140 (MQ=255)           tGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCa                                                                                                                         <  1:938177/140‑1 (MQ=255)             gcgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCa                                                                                                                         <  1:890066/135‑1 (MQ=255)             gcgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCa                                                                                                                         <  2:629581/135‑1 (MQ=255)             gcgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCa                                                                                                                         <  2:1192160/135‑1 (MQ=255)             gcgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCa                                                                                                                         <  1:331149/135‑1 (MQ=255)              cgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCAc                                                                                                                        <  1:247508/136‑1 (MQ=255)              cgagggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAACAATATCAACGAGGTTTTTCGTGCGA‑‑‑GGCGGGTTCTTGAACCCCCAc                                                                                                                        >  2:1161697/5‑140 (MQ=17)                agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTCAACCCCCACGt                                                                                                                      >  1:820870/3‑140 (MQ=255)                         gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                                             <  1:1213801/140‑1 (MQ=255)                           cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                           >  1:49675/1‑140 (MQ=255)                           cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                           >  1:588905/1‑140 (MQ=255)                             tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGGCGCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                         <  1:778218/140‑1 (MQ=255)                                            gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGCGGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                          >  2:211939/1‑140 (MQ=255)                                                gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAGGCTAGcgcg                                                                                      >  1:1170965/1‑140 (MQ=255)                                                  acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                                    >  1:245002/1‑140 (MQ=255)                                                   cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                                   >  1:166919/1‑140 (MQ=255)                                                    acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                  <  1:30390/140‑1 (MQ=255)                                                    acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                  >  2:30390/1‑140 (MQ=255)                                                      tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                <  2:588905/140‑1 (MQ=255)                                                      tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTATTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                <  2:49675/140‑1 (MQ=255)                                                       aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                               <  2:166919/140‑1 (MQ=255)                                                       aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                               <  2:342350/140‑1 (MQ=255)                                                        acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                              >  1:493847/1‑140 (MQ=255)                                                             tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAGGCTAGCGCGTCTACCAATTcca                                                                         >  2:142561/1‑139 (MQ=255)                                                              gAAGCTAGCGCGTCTACCAATTGCGCCACCTTCGCCCAGTGTGAAGAATATCAACGGGGTTTCTCGTGCG‑‑‑CGGGTGGTTCTTGAACACCCACTTCTTTAAGAACACGAACACCTTTACCTAGCGTATCTACAAATTcgac                                                                        >  2:506994/1‑137 (MQ=12)                                                              gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                        <  1:652825/140‑1 (MQ=255)                                                                    aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                  >  2:647251/1‑140 (MQ=255)                                                                         gTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                             <  2:245002/140‑1 (MQ=255)                                                                                aaTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa                                                      <  1:647251/140‑1 (MQ=255)                                                                                 aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                                     <  2:820870/140‑1 (MQ=255)                                                                                  ttCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAGGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt                                                    <  2:1170965/140‑1 (MQ=255)                                                                                   tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                                        <  2:77125/135‑1 (MQ=255)                                                                                   tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                                        >  1:77125/1‑135 (MQ=255)                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                               <  1:1179622/126‑1 (MQ=255)                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                               >  2:1179622/1‑126 (MQ=255)                                                                                     cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCt                                                 >  2:607263/1‑140 (MQ=255)                                                                                      gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                >  1:460691/1‑124 (MQ=255)                                                                                      gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                                <  2:460691/124‑1 (MQ=255)                                                                                       ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACA‑CTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                              >  1:822589/1‑140 (MQ=255)                                                                                        cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                              >  2:1046266/1‑140 (MQ=255)                                                                                             tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                         >  2:577040/1‑140 (MQ=255)                                                                                              cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                        <  1:607263/140‑1 (MQ=255)                                                                                                     tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                                 >  1:612088/1‑140 (MQ=255)                                                                                                     tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                                 >  1:464517/1‑140 (MQ=255)                                                                                                     tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                                 >  1:528690/1‑140 (MQ=255)                                                                                                     tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                                 >  1:360284/1‑140 (MQ=255)                                                                                                        gAGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc                              <  1:577040/140‑1 (MQ=255)                                                                                                         aGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                             >  2:282781/1‑140 (MQ=255)                                                                                                         aGCAATATCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                             >  2:117429/1‑140 (MQ=255)                                                                                                              tatCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                         >  1:446270/1‑139 (MQ=255)                                                                                                              tatCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                         <  2:446270/139‑1 (MQ=255)                                                                                                               atCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       <  1:282781/140‑1 (MQ=255)                                                                                                               atCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       >  2:8605/1‑140 (MQ=255)                                                                                                               atCAACGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       >  2:878396/1‑140 (MQ=255)                                                                                                               atCAACGTGGTTTTTGGGGCGA‑‑‑GGGGGTGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       <  2:493847/140‑1 (MQ=255)                                                                                                                   aCGTGGTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAtt                   >  1:562735/1‑140 (MQ=255)                                                                                                                        gTTTTTGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGt              >  1:264310/1‑140 (MQ=255)                                                                                                                           tttGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGt              >  2:600062/1‑140 (MQ=255)                                                                                                                             tGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       <  2:741447/126‑1 (MQ=255)                                                                                                                             tGGTGCGA‑‑‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                       >  1:741447/1‑126 (MQ=255)                                                                                                                                  cGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACCATCATTTCTTTAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTCTTCAg     >  2:769661/1‑140 (MQ=255)                                                                                                                                       ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCtt  <  1:1161697/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                            CCTCGTTTGGTGC‑GA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTT  >  NC_000913/4606193‑4606462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑