Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	682,156	Δ1 bp	coding (1238/1671 nt)	hscC ←	chaperone protein HscC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	682,153	0	T	.	100.0%	213.7 / NA	51	coding (1241/1671 nt)	hscC	chaperone protein HscC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/25);  total (26/25)

GCCGTCTTCCAGAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGGTTTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCGGAAACGCCCTGGCGGT  >  NC_000913/682014‑682288                                                                                                                                            |                                                                                                                                        gCCGTCTTCCAGAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGGt                                                                                                                                         <  1:1204810/140‑1 (MQ=255) gCCGTCTTCCAGAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGGt                                                                                                                                         <  1:887728/140‑1 (MQ=255)            gAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGAt                                                                                                                             >  2:97665/1‑140 (MQ=255)            gAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGAt                                                                                                                             >  1:555554/1‑140 (MQ=255)             aaGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATa                                                                                                                            <  1:753162/140‑1 (MQ=255)                 aCGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACg                                                                                                                        >  2:796858/1‑140 (MQ=255)                 aCGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACg                                                                                                                        >  2:516112/1‑140 (MQ=255)                  cGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGt                                                                                                                       >  1:676490/1‑140 (MQ=255)                   gTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGtt                                                                                                                      <  1:693466/140‑1 (MQ=255)                    tCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAg                                                                                                                           <  2:987634/134‑1 (MQ=255)                    tCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAg                                                                                                                           >  1:987634/1‑134 (MQ=255)                        cTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCg                                                                                                                 <  1:1118496/140‑1 (MQ=255)                        cTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCg                                                                                                                 <  1:97665/140‑1 (MQ=255)                        cTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCg                                                                                                                 <  1:721165/140‑1 (MQ=255)                             aGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAAtt                                                                                                            >  2:72965/1‑140 (MQ=255)                             aGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAAtt                                                                                                            <  1:72965/140‑1 (MQ=255)                                     gTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTg                                                                                                    >  2:413693/1‑140 (MQ=255)                                      ttGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGt                                                                                                   <  1:89294/140‑1 (MQ=255)                                      ttGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGt                                                                                                   >  1:261788/1‑140 (MQ=255)                                      ttGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGt                                                                                                   >  1:369339/1‑140 (MQ=255)                                         atatCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTcc                                                                                                <  1:521294/140‑1 (MQ=255)                                            tCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAAATGAAt                                                                                                        <  1:520708/129‑1 (MQ=255)                                            tCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAAATGAAt                                                                                                        >  2:520708/1‑129 (MQ=255)                                              aTAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTg                                                                                           >  1:398235/1‑140 (MQ=255)                                               tAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGc                                                                                          <  2:110325/140‑1 (MQ=255)                                                aaCTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGt                                                                                            >  1:30013/1‑137 (MQ=255)                                                aaCTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGt                                                                                            <  2:30013/137‑1 (MQ=255)                                                 aCTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCAt                                                                                        >  2:549419/1‑140 (MQ=255)                                                   tAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATgg                                                                                      <  2:261788/140‑1 (MQ=255)                                                         gAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAAt                                                                                <  2:369339/140‑1 (MQ=255)                                                                     aCTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTacac                                                                    <  2:555554/140‑1 (MQ=255)                                                                      cTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACAcg                                                                   >  2:347833/1‑140 (MQ=255)                                                                         aTAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGa                                                                >  2:955427/1‑140 (MQ=255)                                                                         aTAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGa                                                                >  1:558378/1‑140 (MQ=255)                                                                          tAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGac                                                               >  2:352014/1‑140 (MQ=255)                                                                               cccGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTCCTCGCGACACggg                                                          <  2:829111/140‑1 (MQ=255)                                                                                 cGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGca                                                        <  1:888352/140‑1 (MQ=255)                                                                                 cGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGca                                                        <  1:955427/140‑1 (MQ=255)                                                                                 cGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGca                                                        <  1:896723/140‑1 (MQ=255)                                                                                   gTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGcaca                                                      <  1:549419/140‑1 (MQ=255)                                                                                    ttttCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAg                                                     >  2:168926/1‑140 (MQ=255)                                                                                        cttcAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGcaca                                                      <  2:352457/135‑1 (MQ=255)                                                                                        cttcAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGcaca                                                      >  1:352457/1‑135 (MQ=255)                                                                                             aCGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTc                                            >  1:529191/1‑140 (MQ=255)                                                                                                    aTCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCaat                                     <  2:529191/140‑1 (MQ=255)                                                                                                      cGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCaataa                                   >  1:640772/1‑140 (MQ=255)                                                                                                          ggATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGtt                                              <  2:25482/125‑1 (MQ=255)                                                                                                          ggATTCCACCAGAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGtt                                              >  1:25482/1‑125 (MQ=255)                                                                                                                     gAATGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGc                    >  2:970245/1‑140 (MQ=255)                                                                                                                       aTGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCg                  >  2:1004678/1‑140 (MQ=255)                                                                                                                       aTGTTGTTTTTAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCg                  >  2:971021/1‑140 (MQ=255)                                                                                                                                 tAACTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCGGAAACGCCCt        <  1:796858/140‑1 (MQ=255)                                                                                                                                   aCTTTGTGG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCGGAAACGCCCTgg      <  2:640772/140‑1 (MQ=255)                                                                                                                                       tgtgG‑TTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCGGAAACGCCCTGGCGGt  <  1:516112/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        GCCGTCTTCCAGAAGCACGTCAACTTCAAGCAACCCGTTGATATCATAACTAAAGCGAATATCAATCGACTGATAAGCCCCGGTTTTCTTCAACGGCACATCGAAGGATTCCACCAGAATGTTGTTTTTAACTTTGTGGTTTTCTCCCTGATAGACGTTAACCGTAATTGAATCCTGTTCCGGGTGCATGGTTGAATAAGTTTCTACACGCGACACGGGCACAGTGGTGTTTCGTTCAATAATCGGCGAGAAAATGCCGGAAACGCCCTGGCGGT  >  NC_000913/682014‑682288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑