Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,090	(T)5→4	coding (461/696 nt)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,086	0	T	.	100.0%	204.1 / NA	49	coding (465/696 nt)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/24);  total (25/24)

CGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGA  >  NC_000913/65949‑66218                                                                                                                                          |                                                                                                                                     cGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGttt                                                                                                                                    >  2:528535/1‑140 (MQ=255)   gCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGttttc                                                                                                                                  <  1:912624/140‑2 (MQ=255)   gCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGttttc                                                                                                                                  <  1:48360/140‑2 (MQ=255)    cAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGttttca                                                                                                                                 <  1:939551/140‑3 (MQ=255)            ccccATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTc                                                                                                                        >  1:220860/1‑140 (MQ=255)                atatAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACg                                                                                                                    <  1:528535/140‑1 (MQ=255)                 tataAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGa                                                                                                                   >  2:886822/1‑140 (MQ=255)                  ataAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGAt                                                                                                                  <  2:1029952/140‑1 (MQ=255)                      gCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACg                                                                                                              >  2:653511/1‑140 (MQ=255)                      gCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACg                                                                                                              >  2:341020/1‑140 (MQ=255)                      gCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACg                                                                                                              >  1:1000030/1‑140 (MQ=255)                        gACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTc                                                                                                                        >  2:1007944/1‑128 (MQ=255)                        gACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTc                                                                                                                        <  1:1007944/128‑1 (MQ=255)                          ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGTAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTAc                                                                                                          <  2:845652/140‑1 (MQ=255)                          ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTAc                                                                                                          >  2:441977/1‑140 (MQ=255)                           ctctTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTAcc                                                                                                         >  1:673348/1‑140 (MQ=255)                                ccAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGttt                                                                                                    <  2:288583/140‑1 (MQ=255)                                    cACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCa                                                                                                >  1:177851/1‑140 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCa                                                                                                >  1:829998/1‑136 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCa                                                                                                <  2:829998/136‑1 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa                                                                                            >  1:686196/1‑140 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa                                                                                            >  2:336641/1‑140 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa                                                                                            >  2:334329/1‑140 (MQ=255)                                        aTGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa                                                                                            >  2:7190/1‑140 (MQ=255)                                            cGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATAtt                                                                                        <  1:441977/140‑1 (MQ=255)                                                aTGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGcc                                                                                    <  2:297589/140‑1 (MQ=255)                                                 tGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCg                                                                                   >  1:773915/1‑140 (MQ=255)                                                   cACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGtt                                                                                 <  1:273504/140‑1 (MQ=255)                                                        cATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGAttt                                                                            >  1:1161169/1‑140 (MQ=255)                                                              cGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGt                                                                      <  2:1000030/140‑1 (MQ=255)                                                                gCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCg                                                                    >  2:1068032/1‑140 (MQ=255)                                                                          cccATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCTTTGATTTCTGCGTCGGTCATTTTGc                                                          <  2:1134997/140‑1 (MQ=255)                                                                          cccATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGc                                                          <  1:776527/140‑1 (MQ=255)                                                                           ccATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCg                                                         <  2:137821/140‑1 (MQ=255)                                                                            cATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg                                                        <  2:773915/140‑1 (MQ=255)                                                                            cATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg                                                        <  1:9940/140‑1 (MQ=255)                                                                            cATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg                                                        <  2:646286/140‑1 (MQ=255)                                                                              tGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg                                                        <  1:680201/138‑1 (MQ=255)                                                                              tGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCgg                                                        >  2:680201/1‑138 (MQ=255)                                                                                        ccGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTTCCGGTTTCCCGCTCATATTCGCCGATGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATg                                            <  1:1068032/140‑1 (MQ=255)                                                                                        ccGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATg                                            >  1:298813/1‑140 (MQ=255)                                                                                          gTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGt                                          <  2:181872/140‑1 (MQ=255)                                                                                          gTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGt                                          <  2:298813/140‑1 (MQ=255)                                                                                          gTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGt                                          <  1:886822/140‑1 (MQ=255)                                                                                           tGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTg                                         >  1:1058190/1‑140 (MQ=255)                                                                                            gggAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGc                                        <  2:220860/140‑1 (MQ=255)                                                                                                      cAGAACGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATa                              >  1:980790/1‑140 (MQ=255)                                                                                                           cGCCGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCgg                         <  1:334329/140‑1 (MQ=255)                                                                                                              cGGGCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCgt                      >  2:595698/1‑140 (MQ=255)                                                                                                                 gCATTTGCGCTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCgtggg                   >  2:405213/1‑140 (MQ=255)                                                                                                                         gcTGCATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCg           <  2:1161169/140‑1 (MQ=255)                                                                                                                             cATCGATACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTg       >  2:801639/1‑140 (MQ=255)                                                                                                                                  aTACCCTG‑TTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGa  >  1:1112490/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGA  >  NC_000913/65949‑66218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑