Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,312	(G)5→4	coding (694/702 nt)	lolD →	lipoprotein release complex ‑ ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,308	0	G	.	100.0%	156.6 / NA	38	coding (690/702 nt)	lolD	lipoprotein release complex ‑ ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (17/21);  total (17/21)

TCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGA  >  NC_000913/1177172‑1177443                                                                                                                                         |                                                                                                                                        tCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATgggg                                                                                                                                      <  1:19857/140‑1 (MQ=255) tCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATgggg                                                                                                                                      <  1:151414/140‑1 (MQ=255)    aGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATggggcgg                                                                                                                                   >  1:883065/1‑137 (MQ=255)        gCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTa                                                                                                                              <  2:1058639/140‑1 (MQ=255)           tGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATg                                                                                                                           >  2:197175/1‑140 (MQ=255)                  ttGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGc                                                                                                                    >  2:705097/1‑140 (MQ=255)                    gAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCt                                                                                                                  <  2:442411/140‑1 (MQ=255)                    gAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCt                                                                                                                  <  2:1115659/140‑1 (MQ=255)                       tCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGTCTTTa                                                                                                               >  1:1150329/1‑140 (MQ=255)                        cGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGTCTTTAt                                                                                                              <  2:1150329/140‑1 (MQ=255)                          cTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCg                                                                                                            <  1:984489/140‑1 (MQ=255)                                      cGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGc                                                                                                <  1:197175/140‑1 (MQ=255)                                       gCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGcc                                                                                               <  2:883065/140‑1 (MQ=255)                                        ccTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCt                                                                                              >  2:339780/1‑140 (MQ=255)                                           tCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCg                                                                                           >  1:91753/1‑140 (MQ=255)                                           tCCTGGTGGTTACTCACAACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCg                                                                                           >  2:19434/1‑140 (MQ=255)                                                   gtTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTc                                                                                   >  1:689834/1‑140 (MQ=255)                                                      aCTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTcgcg                                                                                <  1:849071/140‑1 (MQ=255)                                                             aCCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGAcggcg                                                                         >  1:1084766/1‑140 (MQ=255)                                                              ccTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGAcggcgg                                                                        <  1:189561/140‑1 (MQ=255)                                                                  cAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGgcggcgcg                                                                    >  2:71898/1‑140 (MQ=255)                                                                   aaCTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGgcggcgcgg                                                                   <  1:1087409/140‑1 (MQ=255)                                                                        gCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCa                                                              <  1:339780/140‑1 (MQ=255)                                                                           aaaCGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATgg                                                           <  1:71898/140‑1 (MQ=255)                                                                                  tGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt                                                    >  2:535673/1‑140 (MQ=255)                                                                                  tGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt                                                    >  2:106080/1‑140 (MQ=255)                                                                                  tGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACCGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt                                                    <  1:106080/140‑1 (MQ=255)                                                                                     gccgccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGAt                                                 <  1:672472/140‑1 (MQ=255)                                                                                         ccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt                                                    <  1:643087/133‑1 (MQ=255)                                                                                         ccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt                                                    >  2:643087/1‑133 (MQ=255)                                                                                         ccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTcc                                             >  1:93976/1‑140 (MQ=255)                                                                                          cAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTcca                                            <  1:598419/140‑2 (MQ=255)                                                                                          cAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCg                                            <  1:19434/140‑1 (MQ=255)                                                                                             ttAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTa                                         >  1:828545/1‑140 (MQ=255)                                                                                              tAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTAt                                        <  2:848049/140‑1 (MQ=255)                                                                                                  aaTGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCt                                    <  1:535673/140‑1 (MQ=255)                                                                                                      cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCa                                >  2:1109309/1‑140 (MQ=255)                                                                                                       gTGATGGTCGTCTGACGGCGGAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCAt                               >  1:909703/1‑140 (MQ=255)                                                                                                                           gAACTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTgg           <  2:91753/140‑1 (MQ=255)                                                                                                                            aaCTGAGCCTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGc          >  2:14505/1‑140 (MQ=255)                                                                                                                                    cTGAT‑GGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGCCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGa  <  2:1084766/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGA  >  NC_000913/1177172‑1177443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑