Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,608	(C)5→4	coding (372/1359 nt)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,604	0	C	.	100.0%	196.2 / NA	47	coding (376/1359 nt)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/26);  total (21/26)

CGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTC  >  NC_000913/1853469‑1853736                                                                                                                                        |                                                                                                                                     cGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGcccca                                                                                                                                  <  2:188476/140‑2 (MQ=255)  gATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGccccat                                                                                                                                 >  2:1017447/1‑138 (MQ=255)    tAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGccccattc                                                                                                                               >  1:1056297/1‑136 (MQ=255)           cacGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGccc                                                                                                                                    >  2:91011/1‑128 (MQ=255)           cacGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGccc                                                                                                                                    <  1:91011/128‑1 (MQ=255)             cGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCa                                                                                                                     >  1:411160/1‑140 (MQ=255)                   tCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATTAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGa                                                                                                               >  2:1112760/1‑140 (MQ=255)                      ccgcAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAgt                                                                                                            <  2:537748/140‑2 (MQ=255)                        gcAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGa                                                                                                          >  2:191389/1‑140 (MQ=255)                               gCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                                                                                   <  1:830760/140‑1 (MQ=255)                               gCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                                                                                   <  2:610342/140‑1 (MQ=255)                               gCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                                                                                   <  1:80360/140‑1 (MQ=255)                               gCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAagaaga                                                                                                   <  2:116656/140‑1 (MQ=255)                                cATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGAt                                                                                                  >  2:742737/1‑140 (MQ=255)                                    ggCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGc                                                                                              <  2:1085042/140‑1 (MQ=255)                                    ggCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGc                                                                                              <  1:191389/140‑1 (MQ=255)                                        aCCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGt                                                                                          >  2:544468/1‑140 (MQ=255)                                         ccAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTa                                                                                         <  2:1155811/140‑1 (MQ=255)                                                    caaATTAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGa                                                                              <  1:1112760/140‑1 (MQ=255)                                                       aTGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGa                                                                              <  1:604666/137‑1 (MQ=255)                                                       aTGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGa                                                                              >  2:604666/1‑137 (MQ=255)                                                              ccGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGc                                                                    >  1:60539/1‑140 (MQ=255)                                                              ccGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGc                                                                    <  1:878896/140‑1 (MQ=255)                                                              ccGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGc                                                                    >  2:764137/1‑140 (MQ=255)                                                               cgcgcgGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGcc                                                                   <  2:411160/140‑1 (MQ=255)                                                               cgcgcgGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGcc                                                                   <  1:257100/140‑1 (MQ=255)                                                                 cgcgGACCATTTTCCGCGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGcccc                                                                 >  1:855205/1‑140 (MQ=255)                                                                 cgcgGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGcccc                                                                 >  2:1050709/1‑140 (MQ=255)                                                                    ggACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGt                                                              <  2:1123543/140‑1 (MQ=255)                                                                     gACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGtt                                                             <  2:834622/140‑1 (MQ=255)                                                                                   cACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTTTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTGCATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACcgac                                               <  2:536385/140‑1 (MQ=255)                                                                                    aCCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACcgacg                                              <  1:596073/140‑1 (MQ=255)                                                                                     ccGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACcgacga                                             >  1:51450/1‑140 (MQ=255)                                                                                      cGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGAt                                            <  1:544468/140‑1 (MQ=255)                                                                                        tCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATga                                          <  1:1017447/140‑1 (MQ=255)                                                                                        tCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATga                                          >  2:488046/1‑140 (MQ=255)                                                                                                        cGTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGaaa                          >  2:888887/1‑140 (MQ=255)                                                                                                         gTAAATGAGGCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAAc                         >  2:272101/1‑140 (MQ=255)                                                                                                                 ggCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTgcg                 <  2:283091/140‑1 (MQ=255)                                                                                                                  gCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGc                       >  1:58843/1‑133 (MQ=255)                                                                                                                  gCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGc                       <  2:58843/133‑1 (MQ=255)                                                                                                                  gCATAGCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTgcgc                >  1:204624/1‑140 (MQ=255)                                                                                                                       gCCAACCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGccc           >  1:831753/1‑140 (MQ=255)                                                                                                                          aaCCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCaaa        >  1:792744/1‑140 (MQ=255)                                                                                                                           aCCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAag       >  1:733358/1‑140 (MQ=255)                                                                                                                           aCCATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGCGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAag       <  2:1056297/140‑1 (MQ=255)                                                                                                                            ccATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGcgccg              <  1:669036/132‑1 (MQ=255)                                                                                                                            ccATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGcgccg              >  2:669036/1‑132 (MQ=255)                                                                                                                            ccATAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAagt      <  1:776795/140‑1 (MQ=255)                                                                                                                               tAATCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAagtagt   <  2:792744/140‑1 (MQ=255)                                                                                                                                aaTCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTc  <  1:1050709/140‑1 (MQ=255)                                                                                                                                 aTCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTgcg                 <  1:453015/124‑1 (MQ=255)                                                                                                                                 aTCAGCG‑CCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTgcg                 >  2:453015/1‑124 (MQ=255)                                                                                                                                        |                                                                                                                                     CGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGGACCATTTTCCACGCACCGTCGCGGGGATAAACTCCGTAAATGAGGCATAGCCAACCATAATCAGCGCCCCCATTCCTGTTCCCATCAGGAAGCGAAAGAAGATGAGCCAGTACATATCAGGGACAAAAGCGGCCCCTGTTGCAGCAATACCGACGATGAGAAGATTTATGCGAAACGCCCTGCGCCGCCCAAAGTAGTC  >  NC_000913/1853469‑1853736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑