Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,981,526	(C)5→4	coding (87/1425 nt)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,981,522	0	C	.	100.0%	252.3 / NA	60	coding (91/1425 nt)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/35);  total (25/35)

GTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGC  >  NC_000913/1981385‑1981652                                                                                                                                          |                                                                                                                                   gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGccc                                                                                                                                  <  1:503333/140‑1 (MQ=255) gtaTTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGccc                                                                                                                                  <  1:110833/140‑1 (MQ=255)     tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGccccagt                                                                                                                              <  1:557926/140‑4 (MQ=255)     tCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGccccagt                                                                                                                              >  1:971492/1‑137 (MQ=255)         cAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGc                                                                                                                         >  2:681439/1‑140 (MQ=255)         cAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGc                                                                                                                         >  2:935622/1‑140 (MQ=255)          aaGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGcc                                                                                                                        <  1:347820/140‑1 (MQ=255)            ggTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCaa                                                                                                                      >  2:181478/1‑140 (MQ=255)                cTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGc                                                                                                                  >  1:635750/1‑140 (MQ=255)                cTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGc                                                                                                                  >  1:582933/1‑140 (MQ=255)                 tGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCa                                                                                                                 <  1:181478/140‑1 (MQ=255)                   ttCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAg                                                                                                               <  1:11653/140‑1 (MQ=255)                         gAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCAcc                                                                                                         <  1:1010316/140‑1 (MQ=255)                           ggTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCgg                                                                                                       <  2:790537/140‑1 (MQ=255)                               aaaaGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACt                                                                                                   <  2:582933/140‑1 (MQ=255)                                   gagGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTggcg                                                                                               <  1:1018835/140‑1 (MQ=255)                                      gCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGgcggcg                                                                                            >  1:158737/1‑140 (MQ=255)                                        ccACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTg                                                                                          <  2:988999/140‑1 (MQ=255)                                           cGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTc                                                                                       >  2:584287/1‑140 (MQ=255)                                             tAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGACGGCGTGCTCGt                                                                                     <  2:1142751/140‑1 (MQ=255)                                                 gTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtgg                                                                                 >  1:685270/1‑140 (MQ=255)                                                   tACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtggtg                                                                               <  1:656296/140‑1 (MQ=255)                                                    aCCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCtggtgg                                                                              >  2:783921/1‑140 (MQ=255)                                                     ccTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTggtggt                                                                             >  1:222734/1‑140 (MQ=255)                                                     ccTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTggtggt                                                                             <  2:158737/140‑1 (MQ=255)                                                     ccTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTggtggt                                                                             >  2:61538/1‑140 (MQ=255)                                                      cTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTggtggta                                                                            <  2:1088063/140‑2 (MQ=255)                                                      cTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGgtggtg                                                                            <  2:250346/140‑1 (MQ=255)                                                           ttCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATc                                                                       <  2:592427/140‑1 (MQ=255)                                                                   ttttAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGa                                                               <  1:935622/140‑1 (MQ=255)                                                                   ttttAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGa                                                               <  1:681439/140‑1 (MQ=255)                                                                      tAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGAtac                                                            <  2:133/140‑1 (MQ=255)                                                                                tCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTaaa                                                  <  2:635750/140‑1 (MQ=255)                                                                                tCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTaaa                                                  <  2:971492/140‑1 (MQ=255)                                                                                     aTTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGa                                                               >  1:910575/1‑122 (MQ=255)                                                                                     aTTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGa                                                               <  2:910575/122‑1 (MQ=255)                                                                                     aTTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGAtac                                                            <  2:487200/125‑1 (MQ=255)                                                                                     aTTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGAtac                                                            >  1:487200/1‑125 (MQ=255)                                                                                      ttCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTc                                            <  1:1205633/140‑1 (MQ=255)                                                                                         ccTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATa                                         >  1:1102137/1‑140 (MQ=255)                                                                                         ccTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATa                                         >  2:452707/1‑140 (MQ=255)                                                                                         ccTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATa                                         >  2:368874/1‑140 (MQ=255)                                                                                           tGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGt                                       >  2:208884/1‑140 (MQ=255)                                                                                           tGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGt                                       <  2:66711/140‑1 (MQ=255)                                                                                              ttCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAAc                                                 >  1:135637/1‑127 (MQ=255)                                                                                              ttCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAAc                                                 <  2:135637/127‑1 (MQ=255)                                                                                              ttCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGCCTCATAGtcat                                    <  2:222734/140‑1 (MQ=255)                                                                                              ttCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcat                                    <  1:452707/140‑1 (MQ=255)                                                                                               tCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcatc                                   <  1:584287/140‑1 (MQ=255)                                                                                               tCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcatc                                   <  2:685270/140‑1 (MQ=255)                                                                                               tCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGt                                       >  1:221367/1‑136 (MQ=255)                                                                                               tCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGt                                       <  2:221367/136‑1 (MQ=255)                                                                                                caccacTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcatca                                  <  1:368874/140‑1 (MQ=255)                                                                                                   cacTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTc                               >  2:614811/1‑140 (MQ=255)                                                                                                    acTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTcc                              >  1:327635/1‑140 (MQ=255)                                                                                                            cAAACCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTAttt                      >  1:71040/1‑140 (MQ=255)                                                                                                             aaaCCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcat                                    >  1:799000/1‑125 (MQ=255)                                                                                                             aaaCCACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGtcat                                    <  2:799000/125‑1 (MQ=255)                                                                                                                ccACAGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTtctc                  >  2:959128/1‑140 (MQ=255)                                                                                                                    aGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCtttt              <  2:508184/140‑1 (MQ=255)                                                                                                                    aGTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCtttt              <  1:614811/140‑1 (MQ=255)                                                                                                                     gTCCGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCttttt             <  2:549232/140‑1 (MQ=255)                                                                                                                        cGCCTGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTttgt          >  2:838698/1‑140 (MQ=255)                                                                                                                            tGCGGCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTa      <  1:61538/140‑1 (MQ=255)                                                                                                                                gCTTTCAGTG‑CCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGc  <  1:783921/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   GTATTCGTCAAGGTCCTGTTCGCTGAGGTTAAAAGAGGCCCACGTAATGTTACCTTTTTTCACCTTTTTTAGCGGCTGATCCTCATTCCCTGTTTCACCACTCCAGCCAAACCACAGTCCGCCTGCGGCTTTCAGTGCCCCCAGTATGCCAACGGCAAGGCCACCGGCACTGGCGGCGTGCTCGTCTGGTGGTGCAATCCGGTTAGATACTACGACTAAACGACTCATAGTCATCACTCCTGTTATTTTCTCTTTTTTGTTGTAATGC  >  NC_000913/1981385‑1981652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑