Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,918	(C)5→6	coding (45/963 nt)	menC ←	o‑succinylbenzoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,913	1	.	C	100.0%	116.8 / NA	34	V17G (GTG→GGG)	menC	o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (15/19);  total (15/19)

CTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCA‑CCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATG  >  NC_000913/2375781‑2376046                                                                                                                                      |                                                                                                                                      cTTTGCGCCTCTACCCGTGTTTCCTGGCTGAAGCCCGGCTGGGGGGAGTTCCCCCCCCACCCTCCGCGTGCTCCTTCACGCAGAAAAACACACGGCCCGGCGCGGGTTTTTACCCGCCTGTCGCGCAGAACCA‑ccccccg                                                                                                                                <  2:230179/140‑3 (MQ=255)  tttGCGCCTCTTCCCAGGTTTCCTGACGGAAGCCGGGCAGGGGGGAGACCCCCCCCCCCCCTTCGCGCTCGCCTTCCCGCGGGCAAACATACAGCCCGTCGGGGGTTTTTAACCGCCTGTCGCGCAGAACCA‑ccccccgc                                                                                                                               <  2:949447/140‑4 (MQ=255)   ttGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCA‑ccccccgcg                                                                                                                              >  2:424067/1‑136 (MQ=255)   ttGCGCCTCTCCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCCCCCCCCCCCCTTCGCGCTCGCCTTCCCGCAGGCAAACATACAGCCCGGCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCA‑ccccccgcg                                                                                                                              <  2:258650/140‑5 (MQ=255)            ttCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATgggg                                                                                                                      >  2:791664/1‑140 (MQ=255)              cccAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCCCCCCCCCCCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGTTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGAt                                                                                                                    <  2:953963/140‑1 (MQ=255)                   gTTTCCTGCCCGAAGCCCGGCAGGGGGGAGATCCCCCCCCCCCCTTCGCGCCCGCCTTCCCGCAGGAAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGTATCTGcc                                                                                                               <  2:1164010/140‑1 (MQ=255)                     ttCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAg                                                                                                             <  1:791664/140‑1 (MQ=255)                        ctgactgaAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCgg                                                                                                          >  1:805046/1‑140 (MQ=255)                                 gCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTgc                                                                                                 >  1:301719/1‑140 (MQ=255)                                    cGGCAGGGGGGCGACCTCCCCCCCCCCTGCGCGCTCGCCTCCCCGCAGGCAAACATACAGCCCGTCGGGGTTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTgcgcg                                                                                              <  2:1093237/140‑1 (MQ=255)                                     ggCAGTGGGGAGATCCCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTgcgcgc                                                                                             <  1:788216/140‑1 (MQ=255)                                      gCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCt                                                                                            >  2:79920/1‑140 (MQ=255)                                       cggTGGGGAGATCCCCCCCCACCCTTCGCGCTCGCCTTCACGCAGACAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTa                                                                                           <  2:716961/138‑1 (MQ=255)                                                      ccccccccCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTAc                                                                                          <  1:504657/126‑1 (MQ=255)                                                      cccccACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTAc                                                                                          >  2:504657/1‑126 (MQ=255)                                                      cccccACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTcc                                                                            >  2:507242/1‑140 (MQ=255)                                                           aCCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTg                                                                       >  1:723130/1‑140 (MQ=255)                                                           aCCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTg                                                                       >  2:343000/1‑140 (MQ=255)                                                                       cGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAg                                                           >  2:438984/1‑140 (MQ=255)                                                                       cGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAg                                                           >  2:182306/1‑140 (MQ=255)                                                                        cccTTCACGCGGGCAAACATACGGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGt                                                          <  1:1118326/139‑1 (MQ=255)                                                                                  aGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACg                                                <  2:638890/140‑1 (MQ=255)                                                                                    gCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGtt                                              >  2:943998/1‑140 (MQ=255)                                                                                     aaaaCATACACCCCGTCGGGGTTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGttt                                             <  2:301719/139‑1 (MQ=255)                                                                                                  cGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCg                                >  1:588769/1‑140 (MQ=255)                                                                                                    tCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCgct                              >  1:573741/1‑138 (MQ=255)                                                                                                    tCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGtt                              >  2:501281/1‑140 (MQ=255)                                                                                                    tCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGtt                              >  2:997293/1‑140 (MQ=255)                                                                                                        gggTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGa                          <  1:79920/140‑1 (MQ=255)                                                                                                        gggTTTTTAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGa                          <  1:418335/140‑1 (MQ=255)                                                                                                              ttAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTcc                    <  2:588769/140‑1 (MQ=255)                                                                                                              ttAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTcc                    <  1:343000/140‑1 (MQ=255)                                                                                                              ttAACCGCCTGTCGCGCAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTcc                    <  1:507242/140‑1 (MQ=255)                                                                                                                           gcgcAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCg       <  1:93412/140‑1 (MQ=255)                                                                                                                           gcgcAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCg       <  1:595928/140‑1 (MQ=255)                                                                                                                           gcgcAGAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCg       <  1:501281/140‑1 (MQ=255)                                                                                                                                gAACCACCCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATg  <  2:805046/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCA‑CCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGGTTGAAGGCGTTGCGACCTTCCTGACCTTCTTCCGTCATG  >  NC_000913/2375781‑2376046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑