Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,382	(C)6→7	coding (407/555 nt)	yfcE ←	phosphodiesterase YfcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,376	1	.	C	97.3%	255.1 / ‑0.5	73	coding (413/555 nt)	yfcE	phosphodiesterase YfcE
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (44/27);  total (45/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAAT  >  NC_000913/2419243‑2419507                                                                                                                                       |                                                                                                                                    aaTCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGGAGTGGAAAATCT‑cccccc                                                                                                                               >  1:511042/1‑140 (MQ=255)   tCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑cccccccg                                                                                                                             <  2:566675/140‑3 (MQ=255)   tCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑cccccccg                                                                                                                             <  1:331222/140‑3 (MQ=255)             cgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGcc                                                                                                                    >  1:618402/1‑140 (MQ=255)             cgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGATTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGcc                                                                                                                    <  2:410646/140‑1 (MQ=255)              gcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCa                                                                                                                   <  1:149592/140‑1 (MQ=255)                     tACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                            <  1:576034/140‑1 (MQ=255)                      aCTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGtt                                                                                                                         >  2:725219/1‑126 (MQ=255)                      aCTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGtt                                                                                                                         <  1:725219/126‑1 (MQ=255)                       cTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGAt                                                                                                          >  1:1133194/1‑140 (MQ=255)                       cTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGAt                                                                                                          >  1:158899/1‑140 (MQ=255)                          tGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCAc                                                                                                                  <  1:564339/129‑1 (MQ=255)                          tGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCAc                                                                                                                  >  2:564339/1‑129 (MQ=255)                          tGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATggg                                                                                                       >  2:459433/1‑140 (MQ=255)                            aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTa                                                                                                     <  1:405704/140‑1 (MQ=255)                            aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTa                                                                                                     >  1:879303/1‑140 (MQ=255)                            aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTa                                                                                                     <  2:879303/140‑1 (MQ=255)                             tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt                                                                                                    <  1:934458/140‑1 (MQ=255)                             tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt                                                                                                    <  1:65263/140‑1 (MQ=255)                             tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt                                                                                                    <  1:1067228/140‑1 (MQ=255)                                     tGCGATGACACTAAGGACGTCATTATCCAGCATGTCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGCTGAAGCGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTac                                                                                            >  2:857329/1‑140 (MQ=255)                                      gCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTaca                                                                                           >  2:258047/1‑140 (MQ=255)                                       cGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                            >  2:113771/1‑122 (MQ=255)                                       cGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAg                                                                                                            <  1:113771/122‑1 (MQ=255)                                       cGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTacac                                                                                          >  1:346807/1‑140 (MQ=255)                                         aTTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCa                                                                                        <  1:352894/140‑1 (MQ=255)                                                tAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTa                                                                                             >  1:225177/1‑127 (MQ=255)                                                tAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTa                                                                                             <  2:225177/127‑1 (MQ=255)                                                   gTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGtt                                                                              <  2:346807/140‑1 (MQ=255)                                                    tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc                                                                             >  2:935501/1‑140 (MQ=255)                                                    tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc                                                                             <  2:840661/140‑1 (MQ=255)                                                    tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc                                                                             <  2:371061/140‑1 (MQ=255)                                                    tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGAATGACCGTACACCAGCACATCGTTc                                                                             <  2:618402/140‑1 (MQ=255)                                                       gTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGATTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTgg                                                                          >  2:193664/1‑140 (MQ=255)                                                        tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt                                                                         >  1:640443/1‑140 (MQ=255)                                                        tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt                                                                         >  1:210041/1‑140 (MQ=255)                                                          aTTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGttt                                                                       >  1:780818/1‑140 (MQ=255)                                                            tATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa                                                                     >  2:217299/1‑140 (MQ=255)                                                            tATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa                                                                     >  2:288993/1‑140 (MQ=255)                                                            tATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa                                                                     >  1:1187022/1‑140 (MQ=255)                                                                   cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                              >  1:994447/1‑140 (MQ=255)                                                                   cATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcaggc                                                              >  2:160793/1‑140 (MQ=255)                                                                      gCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGa                                                           >  2:331573/1‑140 (MQ=255)                                                                        cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtt                                                         >  2:241762/1‑140 (MQ=255)                                                                        cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtt                                                         >  2:341525/1‑140 (MQ=255)                                                                        cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtt                                                         >  2:357417/1‑140 (MQ=255)                                                                           aaCTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTc                                                      >  2:681366/1‑140 (MQ=255)                                                                                gCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGc                                                 >  1:496224/1‑140 (MQ=255)                                                                                gCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGc                                                 >  2:304776/1‑140 (MQ=255)                                                                                gCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGc                                                 >  2:1015699/1‑140 (MQ=255)                                                                                 ccGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGcc                                                >  1:105909/1‑140 (MQ=255)                                                                                 ccGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGcc                                                <  1:551672/140‑1 (MQ=255)                                                                                  cGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCa                                               <  2:994447/140‑1 (MQ=255)                                                                                    gATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCaaa                                             >  2:1003093/1‑140 (MQ=255)                                                                                     aTTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCaaaa                                            >  2:361157/1‑140 (MQ=255)                                                                                            ccTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGAc                                     <  1:935501/140‑1 (MQ=255)                                                                                                tCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATg                                 <  2:780818/140‑1 (MQ=255)                                                                                                           aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGAGCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                      >  2:979289/1‑140 (MQ=255)                                                                                                           aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                      >  1:406904/1‑140 (MQ=255)                                                                                                           aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                      >  2:729371/1‑140 (MQ=255)                                                                                                           aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                      >  2:1040118/1‑140 (MQ=255)                                                                                                           aCCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa                      >  2:515460/1‑140 (MQ=255)                                                                                                              gAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGAc                   <  1:459433/140‑1 (MQ=255)                                                                                                              gAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGAc                   >  2:894295/1‑140 (MQ=255)                                                                                                              gAGCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGAc                   <  2:1133194/140‑1 (MQ=255)                                                                                                                gCCGGGGTTGGAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgt                 <  2:511042/140‑1 (MQ=255)                                                                                                                gCCGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgt                 >  1:925289/1‑140 (MQ=255)                                                                                                                 ccGGGGTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgtt                >  2:529455/1‑140 (MQ=255)                                                                                                                     ggTTGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGttt            >  1:300002/1‑140 (MQ=255)                                                                                                                        tGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTc         <  2:304213/140‑1 (MQ=255)                                                                                                                        tGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTc         <  1:331573/140‑1 (MQ=255)                                                                                                                        tGAAGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTc         <  2:406904/140‑1 (MQ=255)                                                                                                                          aaGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCa       >  1:409253/1‑140 (MQ=255)                                                                                                                          aaGTGGAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCa       >  1:868966/1‑140 (MQ=255)                                                                                                                              ggAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTaa   <  2:158899/140‑1 (MQ=255)                                                                                                                               gAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAAt  >  2:519435/1‑140 (MQ=255)                                                                                                                               gAAAATCTCCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAAt  >  2:31051/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    AATCGCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCT‑CCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAAT  >  NC_000913/2419243‑2419507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑