Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,682,157	(C)7→8	coding (589/3282 nt)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,682,150	1	.	C	100.0%	135.8 / NA	39	E199G (GAA→GGA)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/16);  total (23/16)

CTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAAC  >  NC_000913/2682031‑2682282                                                                                                                           |                                                                                                                                      cccaagaggAGTAGGCCTCTTAATACTGAGTGCGGATGGCGCGGGGTAAA‑CCGGAGACGTCTCGTTTC‑CCGTCGCCAACTCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCgg                                                                                                                   <  2:509631/133‑1 (MQ=255)      ggggAGTAGCCCTTTTTGTAGTAAGTGCGGGTAGCGGGGGGAAAG‑GGGGAGCCGCCCCGTTTG‑CCGTAACCAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTgg                                                                                                              <  2:73908/138‑1 (MQ=255)       ggggtgagtCCATTTTGTAGTAGGCG‑CGGGAGGGGGGGGGAAA‑GGGGGAGCCGCCCGTTTGCCCGTAAAAAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGc                                                                                                             <  2:33040/134‑1 (MQ=255)            tagtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCccacc                                                                                                        >  1:890858/1‑140 (MQ=255)              gtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCccacaca                                                                                                      >  1:145661/1‑137 (MQ=255)                    tttGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGaaa                                                                                                >  1:500724/1‑140 (MQ=255)                         agtaAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg                                                                                           >  1:59124/1‑140 (MQ=255)                         agtaAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg                                                                                           >  1:703279/1‑140 (MQ=255)                                gCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg                                                                                    >  2:1124431/1‑140 (MQ=255)                                gCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg                                                                                    >  2:1059281/1‑140 (MQ=255)                                     tAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt                                                                               >  1:915920/1‑140 (MQ=255)                                     gtgggggggggAAAGGGGGAGCCGGCCCGTTTG‑CGGTGATAAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggcgt                                                                                <  2:234469/133‑1 (MQ=255)                                     gaGCGATGGGGAAG‑GGGGAGCCGGCCCTTTTG‑CCGTAATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt                                                                               <  1:337553/139‑1 (MQ=255)                                          aTGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCg                                                                           >  2:94081/1‑140 (MQ=255)                                          aTGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCg                                                                           >  2:678631/1‑140 (MQ=255)                                           tGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGtt                                                                         >  1:234469/1‑140 (MQ=255)                                                               ccGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAtt                                                      >  1:1077251/1‑140 (MQ=255)                                                                                      gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc                                >  1:609395/1‑140 (MQ=255)                                                                                      gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc                                >  1:596899/1‑140 (MQ=255)                                                                                       ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                               >  1:753472/1‑140 (MQ=255)                                                                                       ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                               >  2:672969/1‑140 (MQ=255)                                                                                       ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                               <  1:1070250/140‑1 (MQ=255)                                                                                        cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc                              <  2:596899/140‑1 (MQ=255)                                                                                              aTCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGTGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTg                        <  2:1077251/140‑1 (MQ=255)                                                                                               tacggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGa                       <  1:1075274/138‑1 (MQ=255)                                                                                                  ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc                                     <  2:1096121/123‑1 (MQ=255)                                                                                                  ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc                                     >  1:1096121/1‑123 (MQ=255)                                                                                                  ggcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc                    >  2:240563/1‑140 (MQ=255)                                                                                                   gcggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAcc                   >  2:679590/1‑140 (MQ=255)                                                                                                    cggGAAGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCGGGGCGCAGGGTGAAACCt                  >  1:833026/1‑140 (MQ=255)                                                                                                        aaGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtca              >  1:585781/1‑140 (MQ=255)                                                                                                         aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCGGGGCGCAGGGTGAAACCTGtcat             <  2:833026/140‑1 (MQ=255)                                                                                                         aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat             <  2:631224/140‑1 (MQ=255)                                                                                                         aGACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat             <  2:753472/140‑1 (MQ=255)                                                                                                          gACGCTCTGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcatc            >  2:918837/1‑140 (MQ=255)                                                                                                              ctctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat             <  1:1093046/135‑1 (MQ=255)                                                                                                              ctctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat             >  2:1093046/1‑135 (MQ=255)                                                                                                              ctctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCt        <  2:59124/140‑1 (MQ=255)                                                                                                               tctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTg       <  2:890858/140‑1 (MQ=255)                                                                                                                ctGATGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGt      <  2:585781/140‑1 (MQ=255)                                                                                                                    tGCCCTTC‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAAc  <  1:459702/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                      CTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAA‑GCGGAGACGGCCCGTTTG‑CCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAAC  >  NC_000913/2682031‑2682282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑