Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,358	+G	coding (144/474 nt)	trmL →	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,354	1	.	G	91.0%	213.7 / 13.3	67	coding (140/474 nt)	trmL	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base G (43/18);  total (43/24)
Fisher's exact test for biased strand distribution p-value = 1.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

ATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GC‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAT  >  NC_000913/3781215‑3781487                                                                                                                                                |                                                                                                                                       aTGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑c‑gc‑gc                                                                                                                                         <  2:924008/140‑1 (MQ=255) aTGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑c‑gc‑gc                                                                                                                                         <  1:1151705/140‑1 (MQ=255) aTGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑c‑gc‑gc                                                                                                                                         >  1:1192952/1‑140 (MQ=255)  tGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑c‑gc‑gc‑g                                                                                                                                       <  2:569731/140‑1 (MQ=255)     tAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GC‑gggg                                                                                                                                    >  2:838380/1‑140 (MQ=255)          aTCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GC‑gggggctgg                                                                                                                               >  1:1072010/1‑135 (MQ=255)            cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGAc                                                                                                                             >  1:78889/1‑140 (MQ=255)            cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGAc                                                                                                                             >  1:386578/1‑140 (MQ=255)            cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGAc                                                                                                                             >  1:1065083/1‑140 (MQ=255)            cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGAc                                                                                                                             >  1:1153067/1‑140 (MQ=255)            cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGAGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGAc                                                                                                                             >  2:370617/1‑140 (MQ=255)             gTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACt                                                                                                                            <  1:838380/140‑1 (MQ=255)                cTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATc                                                                                                                         <  1:862113/140‑1 (MQ=255)                   tACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACg                                                                                                                      >  2:169731/1‑140 (MQ=255)                   tACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACg                                                                                                                      >  1:451461/1‑140 (MQ=255)                         ccAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTa                                                                                                                >  2:982864/1‑140 (MQ=255)                          cAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTAc                                                                                                               >  2:23333/1‑140 (MQ=255)                           aGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTAcc                                                                                                              >  1:736443/1‑140 (MQ=255)                                  ccGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTa                                                                                                       >  2:624536/1‑140 (MQ=255)                                    cccAAATACTGGCAACATCATCCTTCTTTGCGCTAATACCGGTTTTCGTCTGCATATCACCGAACGGATGGGATTTGCCGGGGACGATAAGCGCCTGCGC‑C‑GG‑GCGGGGGGG‑GGACTATCACGAGTTTACCGCCGTTAcg                                                                                                     <  2:229347/139‑1 (MQ=255)                                     ccAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTAcgc                                                                                                    <  1:370617/140‑1 (MQ=255)                                     caaaaTACTGCAAACATCATCCGTTTTTGCGCTAATCCCGGCTTTCGTCTGCATATCACCGACCCGATGGGATTTGCCTGGGACAATAAGCGCCTGCGC‑C‑GCGGG‑GGGGCT‑GGATTATCACGAGTTTACCGCCGTTAcgc                                                                                                    <  2:1192952/138‑1 (MQ=255)                                        aaTCCTGCCAACATCACCCTTCTTGGCGCTAATCCCGGCTTTCGTCTGCATATCATCGAACCGAGGGGTTTTGCCGGGGACGATAAGCGCTTCCGC‑C‑GC‑GCGGGGGTT‑GGACTATCACGATTTTACCGCCGTTACGCGtc                                                                                                 <  2:233396/140‑1 (MQ=255)                                        aaTACTGGCAACATCATCCGTTTTTGCGCTAATCCCGGCTTTCGTCTGCATATCATCGACCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGtc                                                                                                 <  2:1027585/140‑1 (MQ=255)                                        aaTACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGtc                                                                                                 <  1:1158406/140‑1 (MQ=255)                                               gCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGa                                                                                          >  2:953906/1‑140 (MQ=255)                                               gCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGa                                                                                          >  2:1132731/1‑140 (MQ=255)                                                    atcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATc                                                                                     >  1:487412/1‑140 (MQ=255)                                                       atcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑AC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcg                                                                                  >  1:1072300/1‑140 (MQ=255)                                                         ccGTGTTTGCGATATTGCCGGTTTTCGTCTGCATACGATCGACCCGATGGGATTTCCGGGGGAGGATAGGGGCCTGCGC‑C‑GC‑GCGGGGGTT‑GGACTACCCCATGTTTACCGCCGTTACGCGTCATCATGACTATcgcgcg                                                                                <  2:763694/140‑1 (MQ=255)                                                         ccGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcgcg                                                                                >  1:104312/1‑140 (MQ=255)                                                           gtttttGCGCTAATCCCGGCTTTCGTCTGCATATCATCGACCCGAGGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GCGGG‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGtt                                                                              <  2:1072010/137‑1 (MQ=255)                                                                  cgcTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGaa                                                                       >  1:715301/1‑140 (MQ=255)                                                                  cgcTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGaa                                                                       >  2:462104/1‑140 (MQ=255)                                                                    cTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑AC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGc                                                                     <  2:1072300/140‑1 (MQ=255)                                                                             gCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATcc                                                            >  2:739370/1‑140 (MQ=255)                                                                              cTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGaaa                                                                <  2:9951/135‑1 (MQ=255)                                                                              cTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGaaa                                                                >  1:9951/1‑135 (MQ=255)                                                                                    tCTGCATACCCCCGAACCGATGGGTTTTGCCGGGGACGAAAAGCCCCCGCCC‑CGGG‑GG‑GGGGGT‑GGACTATCCCGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcgc                                                     <  2:68583/140‑1 (MQ=255)                                                                                     cTGCATATCATCGACCCGATGGGTTTTGCCTGGGCCGATAAGCGCCTGCGC‑C‑GCGGG‑GGGGTT‑GGATTACCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGcc                                                    <  2:104312/140‑1 (MQ=255)                                                                                        cATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGt                                                 >  1:142317/1‑140 (MQ=255)                                                                                        cATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGt                                                 >  1:745367/1‑140 (MQ=255)                                                                                           atcatcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCg                                              >  2:1051790/1‑140 (MQ=255)                                                                                           atcatcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCg                                              >  2:154770/1‑140 (MQ=255)                                                                                              atcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGccc                                           >  1:1026169/1‑140 (MQ=255)                                                                                               tcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTctgtct                                          >  1:1046895/1‑135 (MQ=255)                                                                                               tcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCt                                          >  2:969981/1‑140 (MQ=255)                                                                                               tcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCt                                          >  2:62622/1‑140 (MQ=255)                                                                                                cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc                                            <  1:330027/137‑1 (MQ=255)                                                                                                cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGcc                                            >  2:330027/1‑137 (MQ=255)                                                                                                cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTc                                         >  2:280972/1‑140 (MQ=255)                                                                                                  aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcac                                       >  2:199474/1‑140 (MQ=255)                                                                                                  aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcac                                       >  2:912936/1‑140 (MQ=255)                                                                                                   aCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcacc                                      >  1:642861/1‑140 (MQ=255)                                                                                                      gATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg                                   <  2:1026169/140‑1 (MQ=255)                                                                                                      gATGGGATTTGCCGGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg                                   <  1:15723/140‑1 (MQ=255)                                                                                                       aTGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGa                                  >  2:557589/1‑140 (MQ=255)                                                                                                       aTGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGa                                  >  2:765275/1‑140 (MQ=255)                                                                                                          ggATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAg                               >  1:59445/1‑140 (MQ=255)                                                                                                           gATTTGCTGGGGACGATAAGCGCCTGCGC‑C‑GCGGG‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg                              <  1:958841/140‑1 (MQ=255)                                                                                                           gATTTGCCGGGGACGATAAGCGCTTGCGC‑C‑GC‑GCGGGGGCT‑GGATTATCACGTGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg                              <  1:982864/140‑1 (MQ=255)                                                                                                             tttGCTGGGGACAATAAGCGCCTGCGCGG‑GG‑GG‑GGGGCG‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTa                            <  2:745367/140‑1 (MQ=255)                                                                                                             tttGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTa                            <  1:1132731/140‑1 (MQ=255)                                                                                                               tGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTaca                          <  1:23333/140‑1 (MQ=255)                                                                                                                gCCTGGGAGGATAAGCGCCTGCGC‑C‑GCGGG‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTacac                         <  1:149260/140‑1 (MQ=255)                                                                                                                     ggACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCt                    >  2:938202/1‑140 (MQ=255)                                                                                                                     ggACGATAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCt                    >  2:649729/1‑140 (MQ=255)                                                                                                                          aTAAGCGCCTGCGC‑C‑GG‑GC‑GGGGGTGGGTTTATCACGGTTTTTCCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCCCCACGAAAGGTACACCTGCTCACAg               <  2:736443/140‑1 (MQ=255)                                                                                                                           tAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAgc              >  2:448950/1‑140 (MQ=255)                                                                                                                           tAAGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTACTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAgc              >  2:38553/1‑140 (MQ=255)                                                                                                                            aaGCGCCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAgcg             >  2:691821/1‑140 (MQ=255)                                                                                                                             aGCGCCTGCGC‑C‑GCGGG‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAgcgc            <  1:1157129/140‑1 (MQ=255)                                                                                                                               cgcCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCg          <  1:953906/140‑1 (MQ=255)                                                                                                                                gcCTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGt         <  1:938202/140‑1 (MQ=255)                                                                                                                                 ccTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTa        >  2:498250/1‑140 (MQ=255)                                                                                                                                  cTGCGC‑C‑GC‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTaa       <  1:1051790/140‑1 (MQ=255)                                                                                                                                     cgc‑c‑gc‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCt    >  1:152291/1‑140 (MQ=255)                                                                                                                                       c‑c‑gc‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAt  <  1:154770/140‑1 (MQ=255)                                                                                                                                       c‑c‑gc‑GCGGGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAt  <  1:62622/140‑1 (MQ=255)                                                                                                                                                |                                                                                                                                       ATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGC‑C‑GC‑GC‑GGGGCT‑GGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAT  >  NC_000913/3781215‑3781487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑