Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,095,814	(C)6→7	coding (169/1260 nt)	rhaA ←	L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,095,808	1	.	C	100.0%	188.5 / NA	53	I59V (ATT→GTT)	rhaA	L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (27/26);  total (27/26)

ACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAAT‑CCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAAC  >  NC_000913/4095669‑4095939                                                                                                                                             |                                                                                                                                    aCTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAAt                                                                                                                                      <  2:587085/140‑1 (MQ=255) aCTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAAt                                                                                                                                      <  2:524969/140‑1 (MQ=255)            tGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAAT‑cccccccggtc                                                                                                                          <  1:576345/140‑6 (MQ=255)             gATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCa                                                                                                                          <  2:811976/140‑1 (MQ=255)               ttCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGc                                                                                                                        >  2:384754/1‑140 (MQ=255)                        gatGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCt                                                                                                                  <  2:226488/137‑1 (MQ=255)                        gatGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCt                                                                                                                  >  1:226488/1‑137 (MQ=255)                         atGGCATGTAAATTGAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCg                                                                                                              >  1:967365/1‑140 (MQ=255)                         atGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCg                                                                                                              >  1:396887/1‑140 (MQ=255)                          tGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCgg                                                                                                             <  1:21839/140‑1 (MQ=255)                               tGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGtttt                                                                                                        >  1:809170/1‑140 (MQ=255)                               tGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGtttt                                                                                                        >  1:182857/1‑140 (MQ=255)                                 tAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCa                                                                                                      >  1:69420/1‑140 (MQ=255)                                    aTTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCaaaa                                                                                                   <  2:69420/140‑1 (MQ=255)                                    aTTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCaaaa                                                                                                   <  1:1191966/140‑1 (MQ=255)                                     ttAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAAc                                                                                                  <  1:251187/140‑1 (MQ=255)                                       aaGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCg                                                                                                >  1:196281/1‑140 (MQ=255)                                         gCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGa                                                                                              >  2:250061/1‑140 (MQ=255)                                          ccGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGaa                                                                                             >  1:784507/1‑140 (MQ=255)                                               tCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAAcatc                                                                                        <  2:784507/140‑1 (MQ=255)                                                cGGCCCCGGAACCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAAcatca                                                                                       <  2:290115/140‑1 (MQ=255)                                                  gCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACatcatc                                                                                     <  2:967365/140‑1 (MQ=255)                                                  gCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACatcatc                                                                                     <  1:15692/140‑1 (MQ=255)                                                  gCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACatcatc                                                                                     <  2:182857/140‑1 (MQ=255)                                                  gCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACatcatc                                                                                     <  1:250061/140‑1 (MQ=255)                                                  gCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACatcatc                                                                                     <  1:212692/140‑1 (MQ=255)                                                   ccccGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCg                                                                                    >  1:984671/1‑140 (MQ=255)                                                       ggAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGcc                                                                                  <  1:608124/138‑1 (MQ=255)                                                       ggAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGcc                                                                                  >  2:608124/1‑138 (MQ=255)                                                         aaTCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGc                                                                              <  2:379579/140‑1 (MQ=255)                                                              gCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCcagca                                                                         <  2:196281/140‑1 (MQ=255)                                                              gCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCcagca                                                                         <  2:1164194/140‑1 (MQ=255)                                                              gCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCcagca                                                                         <  2:1146027/140‑1 (MQ=255)                                                              gCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCcagca                                                                         <  2:21899/140‑1 (MQ=255)                                                                   aTAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCa                                                                    <  1:196758/140‑1 (MQ=255)                                                                   aTAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCa                                                                    >  1:167776/1‑140 (MQ=255)                                                                                gATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTaa                                                       >  1:589312/1‑140 (MQ=255)                                                                                   cGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTGAACg                                                    >  1:392045/1‑140 (MQ=255)                                                                                      cACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATc                                                 >  1:820704/1‑140 (MQ=255)                                                                                           aGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGtt                                            >  1:918391/1‑140 (MQ=255)                                                                                             cTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTgg                                          >  2:1094532/1‑140 (MQ=255)                                                                                             cTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTgg                                          <  2:701478/140‑1 (MQ=255)                                                                                                aCTGGCATTACGCGCTTTGTCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgcgc                                       >  1:1160709/1‑140 (MQ=255)                                                                                                aCTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGgcgc                                       >  1:347179/1‑140 (MQ=255)                                                                                                    gCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAgcg                                   >  1:96997/1‑140 (MQ=255)                                                                                                      aTTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcc                                 <  2:820704/140‑1 (MQ=255)                                                                                                       ttACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGcct                                >  1:1106358/1‑140 (MQ=255)                                                                                                           gcgcTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCctcctc                            <  2:92812/140‑1 (MQ=255)                                                                                                            cgcTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCg                           >  2:613701/1‑140 (MQ=255)                                                                                                            cgcTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCg                           <  2:984671/140‑1 (MQ=255)                                                                                                              cTTTGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGAc                         >  2:664075/1‑140 (MQ=255)                                                                                                               tttGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACa                        <  1:730930/140‑1 (MQ=255)                                                                                                                ttGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCctcctc                            >  2:1184179/1‑135 (MQ=255)                                                                                                                ttGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCctcctc                            <  1:1184179/135‑1 (MQ=255)                                                                                                                 tGCCCGGATAATTGCCTGTGGCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATc                      >  1:501/1‑140 (MQ=255)                                                                                                                                     gCCTGAATCCCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACTGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAAc  >  2:1161906/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                    ACTGGCGTATCTGATTCCAGATAGATGGCATGTAAATTAAGCCGTTTCGGCCCCGGAATCAGCCGCATAGCCTGTTCCAGATCGGCACGTAGCTCACTGGCATTACGCGCTTTGCCCGGATAATTGCCTGTGGCCTGAAT‑CCCCCCGGTCAGCGAACCTTCCGGGTTTTCAAAACCGGAAACATCATCGCCCTGCCAGCAGTGCATTGAAACGGGTAAACGATCAAGTTGGCGCAGCGCCTCCTCGACATCAATCCCCACCGCCGCGAAAC  >  NC_000913/4095669‑4095939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑