Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,432,376	(G)5→6	coding (681/966 nt)	ytfF ←	inner membrane protein YtfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,432,371	1	.	G	100.0%	214.2 / NA	60	R229P (CGT→CCT)	ytfF	inner membrane protein YtfF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (34/26);  total (34/26)

CTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGG  >  NC_000913/4432234‑4432504                                                                                                                                           |                                                                                                                                      cTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑gg                                                                                                                                     >  1:18328/1‑140 (MQ=255)   gCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑gggg                                                                                                                                   <  1:140686/140‑1 (MQ=255)       gCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcc                                                                                                                               <  1:647613/140‑4 (MQ=255)       gCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcc                                                                                                                               <  2:18328/140‑4 (MQ=255)       gCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcc                                                                                                                               <  2:665328/140‑4 (MQ=255)        cGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcca                                                                                                                              <  1:378557/140‑5 (MQ=255)         gTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggccaa                                                                                                                             <  1:546046/140‑6 (MQ=255)          tttCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCaaa                                                                                                                             >  2:844145/1‑140 (MQ=255)                 aaCAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAgg                                                                                                                      <  2:1164044/140‑1 (MQ=255)                   cAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGga                                                                                                                    >  2:155747/1‑140 (MQ=255)                   cAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGga                                                                                                                    <  1:325392/140‑1 (MQ=255)                      tCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGaa                                                                                                                 >  1:1059860/1‑140 (MQ=255)                      tCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcca                                                                                                                              >  1:1042769/1‑122 (MQ=255)                      tCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑ggggggcca                                                                                                                              <  2:1042769/126‑5 (MQ=255)                              ccGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTc                                                                                                              <  1:382182/135‑1 (MQ=255)                              ccGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTc                                                                                                              >  2:382182/1‑135 (MQ=255)                              ccGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCg                                                                                                         <  2:912267/140‑1 (MQ=255)                              ccGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCg                                                                                                         >  1:569433/1‑140 (MQ=255)                                  gaATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTg                                                                                                     <  2:365295/140‑1 (MQ=255)                                       aCTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTAt                                                                                                >  1:13434/1‑140 (MQ=255)                                        cTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTAtt                                                                                               <  2:569433/140‑1 (MQ=255)                                        cTGTCGGTAATAGCTGGCCGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTAtt                                                                                               <  2:1191896/140‑1 (MQ=255)                                         tGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCGTGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTc                                                                                              >  2:300648/1‑140 (MQ=255)                                             ggTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGcc                                                                                          >  1:1144770/1‑140 (MQ=255)                                                aaTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGt                                                                                       >  2:497557/1‑140 (MQ=255)                                                 aTAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTa                                                                                      >  1:378224/1‑140 (MQ=255)                                                   aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAAc                                                                                    >  1:1177759/1‑140 (MQ=255)                                                   aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAAc                                                                                    >  1:479009/1‑140 (MQ=255)                                                   aGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAAc                                                                                    >  2:879913/1‑140 (MQ=255)                                                    gctggctgGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACa                                                                                   <  2:479009/140‑1 (MQ=255)                                                    gctggctgGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACa                                                                                   <  2:1177759/140‑1 (MQ=255)                                                     ctggctggCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTaa                                                                                     >  2:229254/1‑137 (MQ=255)                                                     ctggctggCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTaa                                                                                     <  1:229254/137‑1 (MQ=255)                                                     ctggctggCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACaa                                                                                  >  1:881697/1‑140 (MQ=255)                                                       ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGc                                                                                >  2:1041844/1‑140 (MQ=255)                                                       ggctggcGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGc                                                                                >  1:448030/1‑140 (MQ=255)                                                              gACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACgaga                                                                         <  2:788744/140‑1 (MQ=255)                                                               aCGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGAt                                                                        <  1:149201/140‑1 (MQ=255)                                                                cGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATa                                                                       <  1:155747/140‑1 (MQ=255)                                                                   tCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGcc                                                                    >  1:613000/1‑140 (MQ=255)                                                                             tGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATGAGTGaa                                                          >  2:902427/1‑140 (MQ=255)                                                                             tGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGaa                                                          >  2:527476/1‑140 (MQ=255)                                                                               cgcCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAAc                                                        <  1:1153896/140‑1 (MQ=255)                                                                                 ccAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCg                                                      <  2:881697/140‑1 (MQ=255)                                                                                 ccAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCg                                                      <  2:13434/140‑1 (MQ=255)                                                                                     cccATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGcc                                                                    <  2:782156/122‑1 (MQ=255)                                                                                     cccATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGcc                                                                    >  1:782156/1‑122 (MQ=255)                                                                                      ccATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATGAGTGAAACCGGCAgt                                                 <  1:902427/140‑1 (MQ=255)                                                                                         tGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGtgtg                                              >  1:714629/1‑140 (MQ=255)                                                                                                aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCg                                                      <  1:1085231/125‑1 (MQ=255)                                                                                                aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAgcg                                       >  1:1217110/1‑140 (MQ=255)                                                                                                aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAgcg                                       >  1:798984/1‑140 (MQ=255)                                                                                                aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAgcg                                       >  1:730686/1‑140 (MQ=255)                                                                                                aGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGAAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCg                                                      >  2:1085231/1‑125 (MQ=255)                                                                                                    cGGCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTg                                   <  1:497557/140‑1 (MQ=255)                                                                                                     ggCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTgc                                  >  2:987437/1‑140 (MQ=255)                                                                                                      gCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTgcg                                 <  2:798984/140‑1 (MQ=255)                                                                                                      gCTATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTgcg                                 <  1:527476/140‑1 (MQ=255)                                                                                                        tATCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCt                               <  2:1052537/140‑1 (MQ=255)                                                                                                         aTCGCAACCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTg                              <  2:724318/140‑1 (MQ=255)                                                                                                               aCCATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGccc                        >  2:312744/1‑140 (MQ=255)                                                                                                                 cATCAGACTAATAAACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCcac                      <  1:987437/140‑1 (MQ=255)                                                                                                                          aataaACACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATAGGa             >  2:224092/1‑140 (MQ=255)                                                                                                                              aaCACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGtt         >  2:573017/1‑140 (MQ=255)                                                                                                                              aaCACCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGtt         >  1:240120/1‑140 (MQ=255)                                                                                                                                 acCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGt      >  2:1096727/1‑140 (MQ=255)                                                                                                                                 acCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTACGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGccc                        <  1:946678/122‑1 (MQ=255)                                                                                                                                 acCAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTACGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGccc                        >  2:946678/1‑122 (MQ=255)                                                                                                                                   cAGCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCg    >  2:171276/1‑140 (MQ=255)                                                                                                                                   cAGCGGACGGGGGGCCAAAGGGTA‑GGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCgg   >  2:1021560/1‑140 (MQ=255)                                                                                                                                     gCGGACGGGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCggg  >  1:959599/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                      CTGCCAGCGTTTCGAAAACAATCAGCGGCCCGAGAATCACTGTCGGTAATAGCTGGCTGGCGACGTTCCAGCAGAGTGCGCCAACCCATGAGCAAAGCACGGCTATCGCAACCATCAGACTAATAAACACCAGCGGAC‑GGGGGCCAAAGGGTAGGGAGAAGTCCGGCGTTTGCGTATTCAGCCAGTAACAAGCGACGAGATAGCCGATAAGTGAAACCGGCAGTGTGACCAGCGCCTGCGCTGTTGCCCACATCATCGGATGTTTGTCGGG  >  NC_000913/4432234‑4432504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑