Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,080,039	(G)5→6	coding (735/1509 nt)	putP →	proline:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,080,034	1	.	G	91.7%	192.3 / 9.8	60	coding (730/1509 nt)	putP	proline:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base G (30/25);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑GGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTG  >  NC_000913/1079895‑1080166                                                                                                                                              |                                                                                                                                     ccTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑tt                                                                                                                                       <  1:121329/140‑1 (MQ=255)     aTCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑gggg                                                                                                                                  >  2:161715/1‑140 (MQ=255)     aTCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑gggg                                                                                                                                  <  2:245003/140‑1 (MQ=255)               cGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTa                                                                                                                         >  2:762475/1‑140 (MQ=255)               cGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑GGGGGCTGGGTTAc                                                                                                                        >  1:313926/1‑140 (MQ=255)                 gTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACt                                                                                                                       >  2:375559/1‑140 (MQ=255)                 gTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACt                                                                                                                       >  1:228785/1‑140 (MQ=255)                 gTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACt                                                                                                                       >  2:544762/1‑140 (MQ=255)                    aTCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCg                                                                                                                    <  2:535460/140‑1 (MQ=255)                                      ggTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTgg                                                                                                  >  1:437360/1‑140 (MQ=255)                                       gTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGgc                                                                                                 <  2:1028479/140‑1 (MQ=255)                                       gTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGgc                                                                                                 <  2:596131/140‑1 (MQ=255)                                                    cTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATggcg                                                                                    >  1:206788/1‑140 (MQ=255)                                                     tCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATggcgg                                                                                   >  1:330295/1‑140 (MQ=255)                                                          ggAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGAt                                                                              >  2:337220/1‑140 (MQ=255)                                                                 aTCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATggcggc                                                                                  >  2:491228/1‑129 (MQ=255)                                                                 aTCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATggcggc                                                                                  <  1:491228/129‑1 (MQ=255)                                                                 aTCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGACAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTcacc                                                                       >  1:1042892/1‑140 (MQ=255)                                                                 aTCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑G‑‑‑‑‑‑GGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTcacc                                                                       >  2:623396/1‑133 (MQ=255)                                                                 aTCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑G‑‑‑‑‑‑GGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTcacc                                                                       <  1:623396/133‑1 (MQ=255)                                                                     aacaaaAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGACAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAg                                                                   <  2:1042892/140‑1 (MQ=255)                                                                     aacaaaAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATAGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGGTTCTCACCACAg                                                                   >  2:1103900/1‑140 (MQ=255)                                                                             agCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTcc                                                           >  2:1069597/1‑140 (MQ=255)                                                                              gCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCa                                                          <  1:1215188/140‑1 (MQ=255)                                                                                aTCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATg                                                        >  2:388763/1‑140 (MQ=255)                                                                                aTCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATg                                                        >  2:441152/1‑140 (MQ=255)                                                                                 tCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgc                                                       >  1:715725/1‑140 (MQ=255)                                                                                  cGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcg                                                      <  1:612001/140‑1 (MQ=255)                                                                                  cGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcg                                                      >  2:612001/1‑140 (MQ=255)                                                                                    aaaaCGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    >  2:49845/1‑140 (MQ=255)                                                                                    aaaaCGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    <  1:762475/140‑1 (MQ=255)                                                                                    aaaaCGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    <  1:161715/140‑1 (MQ=255)                                                                                    aaaaCGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    <  2:330295/140‑1 (MQ=255)                                                                                     aaaCGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGcgt                                                   <  2:715725/140‑1 (MQ=255)                                                                                         gTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTa                                               <  2:437360/140‑1 (MQ=255)                                                                                          ttGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTAt                                              <  1:49845/140‑1 (MQ=255)                                                                                            ggccagCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTa                                            >  1:854200/6‑140 (MQ=255)                                                                                            gATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcg                                                      >  2:215030/1‑130 (MQ=255)                                                                                            gATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcg                                                      <  1:215030/130‑1 (MQ=255)                                                                                              tatGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGt                                          <  2:228785/140‑1 (MQ=255)                                                                                              tatGCTCAAAGGTCTGAACTATGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGt                                          <  2:817000/140‑1 (MQ=255)                                                                                                tGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTAt                                        <  2:232677/140‑1 (MQ=255)                                                                                                     aaaGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGTGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    <  2:564630/123‑1 (MQ=255)                                                                                                     aaaGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGTGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGcgcg                                                    >  1:564630/1‑123 (MQ=255)                                                                                                     aaaGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGGCGTATTAGTATGACCt                                   <  2:206788/140‑1 (MQ=255)                                                                                                        ggTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGga                                <  1:375559/140‑1 (MQ=255)                                                                                                         gTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGgat                               <  1:337220/140‑1 (MQ=255)                                                                                                          tCTGAACTTTGTTGCCATTATCTCACTGATGGGTTT‑GGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGt                                          >  2:702095/1‑128 (MQ=255)                                                                                                          tCTGAACTTTGTTGCCATTATCTCACTGATGGGTTT‑GGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGt                                          <  1:702095/128‑1 (MQ=255)                                                                                                          tCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGgatg                              >  1:565881/1‑140 (MQ=255)                                                                                                            tGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGgatgat                            >  2:961451/1‑140 (MQ=255)                                                                                                            tGAACTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGgatgat                            <  2:1093589/140‑1 (MQ=255)                                                                                                               aCTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGAc                                     >  2:620771/1‑128 (MQ=255)                                                                                                               aCTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGAc                                     <  1:620771/128‑1 (MQ=255)                                                                                                               aCTTTGTTGCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCct                         <  1:961451/140‑1 (MQ=255)                                                                                                                   tgttgCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGc                     >  2:607542/1‑140 (MQ=255)                                                                                                                   tgttgCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGc                     >  1:998312/1‑140 (MQ=255)                                                                                                                   tgttgCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGc                     >  2:844356/1‑140 (MQ=255)                                                                                                                     ttgCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCt                   <  1:300796/140‑1 (MQ=255)                                                                                                                     ttgCCATTATCTCACTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCt                   >  2:300796/1‑140 (MQ=255)                                                                                                                                  aCTGATGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTg      >  2:275280/1‑140 (MQ=255)                                                                                                                                      aTGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTg  >  1:615442/1‑140 (MQ=255)                                                                                                                                      aTGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTg  >  1:75525/1‑140 (MQ=255)                                                                                                                                      aTGGG‑TTGGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGGGGCtt  >  2:179220/1‑139 (MQ=255)                                                                                                                                              |                                                                                                                                     CCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGG‑TT‑GGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTG  >  NC_000913/1079895‑1080166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑