Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,480,639	(A)6→7	coding (1502/1758 nt)	ynbC →	hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,480,633	1	.	A	100.0%	116.0 / NA	34	V499E (GTA→GAA)	ynbC	hydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (14/20);  total (14/20)

CTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑AAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGATGGTAAACCG  >  NC_000913/1480495‑1480763                                                                                                                                            |                                                                                                                                   cTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑a                                                                                                                                   >  2:1116118/1‑140 (MQ=255) cTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑a                                                                                                                                   >  1:968282/1‑140 (MQ=255) cTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑a                                                                                                                                   >  2:261047/1‑140 (MQ=255) cTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑a                                                                                                                                   >  1:456228/1‑140 (MQ=255) cTCAACGGGGAATGTCTGGGCGGGCGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑a                                                                                                                                   >  2:222389/1‑140 (MQ=255)       ggggAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑aaaaaaa                                                                                                                             >  2:267133/1‑139 (MQ=255)          gAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑aaaaaaactc                                                                                                                          <  1:330789/140‑5 (MQ=255)               tCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCg                                                                                                                      <  2:503055/140‑1 (MQ=255)               tCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCg                                                                                                                      <  1:288867/140‑1 (MQ=255)                   ggCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAgg                                                                                                                  >  1:1032235/1‑140 (MQ=255)                        gTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTg                                                                                                             >  2:597588/1‑140 (MQ=255)                         tGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCa                                                                                                                    >  2:178409/1‑132 (MQ=255)                         tGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCa                                                                                                                    <  1:178409/132‑1 (MQ=255)                         tGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGc                                                                                                            >  1:932670/1‑140 (MQ=255)                         tGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGc                                                                                                            >  2:606935/1‑140 (MQ=255)                         tGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGc                                                                                                            >  1:1120953/1‑140 (MQ=255)                          gCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGcc                                                                                                           <  2:1084872/140‑1 (MQ=255)                                      gggCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGaa                                                                                               <  1:346715/140‑1 (MQ=255)                                           aTGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCggg                                                                                          <  2:1184881/140‑1 (MQ=255)                                                tttAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCa                                                                                     <  1:267133/140‑1 (MQ=255)                                                tttAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCa                                                                                     <  2:1077298/140‑1 (MQ=255)                                                 ttAGCCCTGAGGAACTGAGCGCGTTAACTGCGCGGCGTACTCTGGCGATTGCCTCTGGCCTGTATGAGCTTCTTTCCTAAAATGAGCAATC‑AAAACGCCGACACGCAGGTCTTGCTCATCTCCTCCAACCggggggaagt                                                                                   <  2:297826/140‑5 (MQ=255)                                                  tAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCAtt                                                                                   <  2:400567/140‑1 (MQ=255)                                                  tAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCAtt                                                                                   <  1:1116118/140‑1 (MQ=255)                                                                 cAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCggg                                                                    <  1:183769/140‑1 (MQ=255)                                                                                 cgGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTc                                                    <  2:932670/140‑1 (MQ=255)                                                                                  ggCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCa                                                   <  1:416258/140‑1 (MQ=255)                                                                                           tGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAgat                                          <  2:456228/140‑1 (MQ=255)                                                                                           tGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAgat                                          <  2:968282/140‑1 (MQ=255)                                                                                              cGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAgatgat                                       >  2:246238/1‑140 (MQ=255)                                                                                                       ctGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGgt                              >  2:149893/1‑140 (MQ=255)                                                                                                       ctGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGgt                              >  1:641105/1‑140 (MQ=255)                                                                                                               tATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCa                      >  1:470804/1‑140 (MQ=255)                                                                                                               tATGAGCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCa                      >  2:724808/1‑140 (MQ=255)                                                                                                                    gCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAAc                        <  1:433709/133‑1 (MQ=255)                                                                                                                    gCTTTTTCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAAc                        >  2:433709/1‑133 (MQ=255)                                                                                                                      tttttCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATaa               <  1:1139652/140‑1 (MQ=255)                                                                                                                        tttCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAg             <  1:261047/140‑1 (MQ=255)                                                                                                                         ttCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGa            >  2:340397/1‑140 (MQ=255)                                                                                                                         ttCCCGAAAATGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGa            >  1:1159910/1‑140 (MQ=255)                                                                                                                               aaaaTGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGATGGTaa      <  2:470804/140‑1 (MQ=255)                                                                                                                                   tGAGCAGGTAAAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGATGGTAAACCg  <  2:831621/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                   CTCAACGGGGAATGTCTGGGCGGGTGCGTTTTGAACAGGGCGATGCGTTTAACCCGGAGGAACTCAGCGCGTTAACTCCGCGGCCTACGCTGGCGATTGTCTCTGGCCTGTATGAGCTTTTTCCCGAAAATGAGCAGGT‑AAAAAACTCACTCGCAGGTCTTGCCAATGCCATCGAACCGGGCGGCATTCTCATCTACACCGGGCAGCCGTGGCACCCTCAACTGGAGATGATTGCCGGGGTGTTAACCAGTCATAAAGATGGTAAACCG  >  NC_000913/1480495‑1480763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑