| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,904,787 | (T)7→8 | intergenic (+41/‑14) | manZ → / → yobD | mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,904,780 | 1 | . | T | 97.4% | 129.2 / ‑3.5 | 38 | intergenic (+34/‑21) | manZ/yobD | mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (17/20); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑G‑TTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGC > NC_000913/1904649‑1904903 | tgtgGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑G‑tttttttt < 1:319298/140‑2 (MQ=255) gtgGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑G‑tttttttta < 2:520211/140‑3 (MQ=255) tACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTgg < 1:135256/140‑1 (MQ=255) tACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTgg < 2:271751/140‑1 (MQ=255) aCTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTgga < 2:421636/140‑1 (MQ=255) tGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTggagg > 2:1185553/1‑140 (MQ=255) gcgcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTggagga > 1:982014/1‑140 (MQ=255) gcgcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTggagga < 1:959378/140‑1 (MQ=255) aaGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGAc < 2:660313/140‑1 (MQ=255) cTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGAcc < 1:793856/140‑1 (MQ=255) atcatcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTAc < 2:633767/140‑1 (MQ=255) atcatcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTAc < 2:445527/140‑1 (MQ=255) atcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGATGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGa > 1:1062345/1‑140 (MQ=255) ggCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGt > 1:260388/1‑129 (MQ=255) ggCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGt < 2:260388/129‑1 (MQ=255) tcttcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCAt < 1:1109847/140‑1 (MQ=255) tcttcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCAt < 2:550823/140‑1 (MQ=255) ttcGTCATCGGTATAGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcg > 2:534684/1‑140 (MQ=255) gTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 2:982014/140‑1 (MQ=255) gTCATCGGTATAGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 1:534684/140‑1 (MQ=255) cGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTc > 2:666788/1‑140 (MQ=255) gTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCt > 1:684923/1‑140 (MQ=255) gTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCt < 1:666788/140‑1 (MQ=255) gCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCt > 2:722512/1‑140 (MQ=255) tGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCTCTCCTGGCCTTc < 2:834275/140‑1 (MQ=255) ggTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcg < 1:152620/140‑1 (MQ=255) tttccGCTTGCGCCTTGTGGGGATTTTAAGCCTTTTTTCCACTCCGGGGGCT‑TTTTGCCCC‑CTTT‑TTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcg < 2:1190236/136‑1 (MQ=255) gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGTGGATTAATGACAATCACGCACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 2:1084271/140‑1 (MQ=255) gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 1:86771/140‑1 (MQ=255) gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 2:30583/140‑1 (MQ=255) gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 2:184361/140‑1 (MQ=255) cGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcg > 1:628380/1‑129 (MQ=255) cGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcg < 2:628380/129‑1 (MQ=255) cGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGAt > 1:295072/1‑140 (MQ=255) cGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGAt > 2:267889/1‑140 (MQ=255) tgctgGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGtt > 1:1175258/1‑140 (MQ=255) ttgGGACTGTAAGACTGTTGTGCTCTACCGGGCCC‑TTTTGGCCC‑CT‑T‑TTTTTTTATTGGGAGGATTAATGACAATCACGGACCTGCTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTtca < 2:867150/139‑1 (MQ=255) aaGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCccg > 2:52575/1‑140 (MQ=255) ttgtACACTACCGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAAc > 1:644579/1‑140 (MQ=255) ctgcGGGGCCTTTTTGGCCCTT‑‑T‑TTTTTTTATCGGGAGGATTAATAAAAACCCGGGACGTGGTTGTTTTTTTTTCCACCCCCGCCCCCGTGCCCTCCGGTATCTACGATCATTTCCTCTGCCCCCGCCGTAACGGCCCCa < 2:428449/137‑1 (MQ=255) ccGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCAccc > 1:428449/1‑140 (MQ=255) ccGGGGCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCAccc > 2:140567/1‑140 (MQ=255) gggCC‑TTTTGGCCC‑C‑‑GTTTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCctgc > 1:382993/1‑140 (MQ=255) | TGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCC‑TTTTGGCCC‑C‑‑G‑TTTTTTTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGC > NC_000913/1904649‑1904903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |