Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT35.9% 68.6 / 25.4 39N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (23/2);  new base T (0/14);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 7.96e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111621‑4111885
                                                                                                                               |                                                                                                                                          
aTTAAACCCTTCAAGCGATTCAGATAGCCCCAGTTAAATCGGTCCAACAGCGAAGGCCACCCCCTTTC‑CGCCGTTGCCCGCGACAACATACCGCAGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCccgccg                                                                                                                               <  2:1142641/140‑1 (MQ=255)
           cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                    >  1:418559/1‑140 (MQ=255)
           cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                    >  1:278107/1‑140 (MQ=255)
           cAAGCAATTCAGATACCGCCAGTTAAATCGTTTAAACAGCGAAGGTCCCCCCTTTTT‑CGCCGTTGCCCGCAACAAAATAACGCGGTGCCCTTTCTGTCTGGGTGAAATATTGTTTTTTTTCCCCCGCCGTTAGTAGCGAc                                                                                                                    <  2:73743/140‑1 (MQ=255)
                  ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                                                                                             >  2:1157491/1‑140 (MQ=255)
                    cAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGGAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttt                                                                                                           >  2:587208/1‑140 (MQ=255)
                            gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:84027/1‑140 (MQ=255)
                            gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  2:85618/1‑140 (MQ=255)
                                        ggtaaaaCAGCGAAGGCCACCCCTTTTC‑CGCTGTTGCCCGCAACAACAAAACGCAGTGCCCTTTCTGTCTCGGTTTAATATTTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                       <  2:1014083/135‑1 (MQ=255)
                                          ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACGTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  2:840517/1‑140 (MQ=255)
                                          ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:1134713/1‑140 (MQ=255)
                                          ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:750722/1‑140 (MQ=255)
                                          ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:650954/1‑140 (MQ=255)
                                          ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:721391/1‑140 (MQ=255)
                                          tacAACAGCGAAGGTCATCCCCTTTC‑CGCCGTTGCCCGCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATACTTTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     <  2:1150560/138‑1 (MQ=255)
                                           taaaCAGCGAAGGTCACCCCTTTTC‑CGCCGTTGTCCGCAACAACATACCGCAGTGCCCCTTCTGTCTGGGTGTAATAACGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    <  2:750722/138‑1 (MQ=255)
                                           tCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    >  1:302750/1‑140 (MQ=255)
                                           tCAACAGCGAAGCTCAGCCCCTTCT‑CGCCGTTGTCCGCGACAACATAGCGCAGTGCACCTTCTGCGTCGGTGCAATACCGTTTGTTTTTCGCCGCTGATAGGCGCGGCTGCCGTTTTTTCTGGCTTTGTGTTTTGGCCAt                                                                                    >  2:1134509/1‑140 (MQ=255)
                                           ccAACGGCGAAGGTCCCCCCTTTTT‑CGCCGTTCTCCGCAACAACATAAGGCATTGCCCTTTCGTTCTGGTTTTAATAGTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    <  2:1134713/139‑1 (MQ=255)
                                           caaaCAGGGAGGGCCACCCCTTTTC‑CGCCTTTGCCCGCGACAAAATAACGCAGTCCCCTTTCTGTCTGGGTTTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    <  2:721391/138‑1 (MQ=255)
                                              aCAGCGAAGGCCACCCCTTTTT‑CGCCGTTGCCCGCGACAACATAACGCAGTCCCCCTTCTGTCTCGGTTTAATATCTTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                                 <  2:84027/140‑1 (MQ=255)
                                                   gAAGGTCCCCCCTTTTC‑CGCCGTTGCCCGCGACAACATAACGCAGTGCCCCTTTTGTCTCGGTTTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgggg                                                                            <  2:834764/140‑1 (MQ=255)
                                                    aaGGTCCCCCCTTTTT‑CGCCGTTGCCCGCGACAACATAACGCAGTGCCCCTTCTGTCGGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:27707/140‑1 (MQ=255)
                                                    aaGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  2:912488/1‑140 (MQ=255)
                                                         ccaccccTTTTCCGCGGTTGCCCGCAAAAAAATACCGCAGTGCCCCTTCTGTCTGGGTATAATAGCGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt                                                                       <  2:433519/137‑1 (MQ=255)
                                                            ccctTTTC‑CGCCGTTGCCCGCGACAACATAACGCGGTCCCCCTTCTGTCTGGGTGTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc                                                                   <  2:650954/140‑1 (MQ=255)
                                                                tttc‑CGCCGTTGCCCGCAAAAACATAACGCAGTCCCCCTTCTTTCTGGGTGTAATAATTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt                                                               <  2:418559/140‑1 (MQ=255)
                                                                 ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  1:13242/1‑140 (MQ=255)
                                                                 ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGGGAGTGTGCGGTTc                                                              >  1:91344/1‑140 (MQ=255)
                                                                  tt‑CCCCTTTGTCCGCGAAAAAAAAACGCAGTCCCCCTTTTGTCTGGGTTTAATATGTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCa                                                             <  1:887453/140‑1 (MQ=255)
                                                                          ttGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACtgctgc                                                      >  1:4856/1‑140 (MQ=255)
                                                                             tCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCAt                                                   >  1:931156/1‑140 (MQ=255)
                                                                              ccGCGAAAACATACCGCGGTCCCCTTTCTGTCTGGGTTTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                  <  2:278107/140‑1 (MQ=255)
                                                                                       caTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                         >  1:283567/1‑140 (MQ=255)
                                                                                         tAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgc                                       >  1:1156668/1‑140 (MQ=255)
                                                                                            cGCAGTCCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgcttt                                    <  1:85618/140‑1 (MQ=255)
                                                                                                  gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                              >  1:741536/1‑140 (MQ=255)
                                                                                                      tttCTGTCTGGGTTAAATGACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                          <  1:1134509/139‑1 (MQ=255)
                                                                                                           gTCTGGGTTTAATACTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     <  2:125956/140‑1 (MQ=255)
                                                                                                           gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTTGCTTTCGCGGCttt                     >  2:809252/1‑140 (MQ=255)
                                                                                                           gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     >  1:141352/1‑140 (MQ=255)
                                                                                                                ggTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  1:125956/1‑140 (MQ=255)
                                                                                                                 gtgtAATAACGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTTGCTTTCGCGGCTTTTTGctc               <  1:809252/140‑1 (MQ=255)
                                                                                                                 gtgtAATAACGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               <  1:1010869/140‑1 (MQ=255)
                                                                                                                         atttttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCt       <  1:46187/137‑1 (MQ=255)
                                                                                                                          ttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:302750/138‑1 (MQ=255)
                                                                                                                            tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:352231/1‑140 (MQ=255)
                                                                                                                            tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:972736/1‑140 (MQ=255)
                                                                                                                              tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTg                  >  2:626214/1‑124 (MQ=255)
                                                                                                                              tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:688177/1‑140 (MQ=255)
                                                                                                                              tgtttTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTg                  <  1:626214/124‑1 (MQ=255)
                                                                                                                               |                                                                                                                                          
ATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111621‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑