BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	169,469	T→C	G662G (GGT→GGC)	fhuA →	ferrichrome outer membrane transporter/phage receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	169,469	0	T	C	100.0%	453.0 / NA	125	G662G (GGT→GGC)	fhuA	ferrichrome outer membrane transporter/phage receptor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (69/56); total (69/56)

Only 100 of 126 total aligned reads displayed.

AGTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGTCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGG  >  NC_000913/169334‑169596

aGTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGa                                                                                                                               <  2:37905/140‑1 (MQ=255)

 gTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGAc                                                                                                                              >  1:1016416/1‑140 (MQ=255)

 gTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGAc                                                                                                                              >  2:533908/1‑140 (MQ=255)

 gTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGAc                                                                                                                              >  1:211952/1‑140 (MQ=255)

  tCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACg                                                                                                                             >  2:42277/1‑140 (MQ=255)

               ttACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCggtg                                                                                                                >  1:777696/1‑140 (MQ=255)

               ttACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCggtg                                                                                                                <  2:354442/140‑1 (MQ=255)

                tACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACACCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACg                                                                                                                             >  1:202253/1‑126 (MQ=255)

                tACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACACCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACg                                                                                                                             <  2:202253/126‑1 (MQ=255)

                 acacCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCggtggt                                                                                                              >  1:1182114/1‑140 (MQ=255)

                 acacCGATGCGGAATACACCACCGATACTACCTATAAAGGCAAT‑CGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTc                                                                                                             >  1:931161/1‑140 (MQ=255)

                   acCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCg                                                                                                            >  1:1161015/1‑140 (MQ=255)

                   acCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCg                                                                                                            >  1:141464/1‑140 (MQ=255)

                   acCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCg                                                                                                            >  2:708068/1‑140 (MQ=255)

                   acCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCg                                                                                                            >  1:867776/1‑140 (MQ=255)

                    ccGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGt                                                                                                           <  2:1090309/140‑1 (MQ=255)

                      gATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTta                                                                                                         >  1:740360/1‑140 (MQ=255)

                      gATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTta                                                                                                         >  1:183676/1‑140 (MQ=255)

                      gATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTta                                                                                                         >  1:81576/1‑140 (MQ=255)

                       aTGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtat                                                                                                        >  1:114508/1‑140 (MQ=255)

                       aTGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtat                                                                                                        >  1:600453/1‑140 (MQ=255)

                       aTGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtat                                                                                                        >  2:84536/1‑140 (MQ=255)

                        tGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtata                                                                                                       >  2:310554/1‑140 (MQ=255)

                        tGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtata                                                                                                       >  2:1184690/1‑140 (MQ=255)

                          cGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACt                                                                                                     <  2:1161015/140‑1 (MQ=255)

                             aaTACACCACCGATACTACCTCTAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGc                                                                                                  <  2:1107446/140‑1 (MQ=255)

                             aaTACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTGCACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGc                                                                                                  <  2:855631/140‑1 (MQ=255)

                             aaTACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGc                                                                                                  <  2:1091751/140‑1 (MQ=255)

                                acacCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTcc                                                                                               <  1:594595/140‑1 (MQ=255)

                                 caccacCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATAc                                                                                                      <  2:357640/132‑1 (MQ=255)

                                 caccacCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATAc                                                                                                      >  1:357640/1‑132 (MQ=255)

                                  accaccGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAg                                                                                             >  1:549439/1‑140 (MQ=255)

                                    caccGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGtt                                                                                           >  2:1004014/1‑140 (MQ=255)

                                    caccGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGtt                                                                                           >  2:190611/1‑140 (MQ=255)

                                     accGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTa                                                                                          <  2:1016416/140‑1 (MQ=255)

                                      ccGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTAt                                                                                         >  2:881954/1‑140 (MQ=255)

                                       cGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTc                                                                                                <  1:102033/132‑1 (MQ=255)

                                       cGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTc                                                                                                >  2:102033/1‑132 (MQ=255)

                                         aTACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtata                                                                                                       >  2:287246/1‑123 (MQ=255)

                                         aTACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTtata                                                                                                       <  1:287246/123‑1 (MQ=255)

                                         aTACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACt                                                                                                     >  2:455329/1‑125 (MQ=255)

                                         aTACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACt                                                                                                     <  1:455329/125‑1 (MQ=255)

                                         aTACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑Gt                                                                                    >  1:986801/1‑140 (MQ=255)

                                           actacCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGa                                                                                  >  1:1208544/1‑140 (MQ=255)

                                            ctacCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGAt                                                                                 <  2:1182114/140‑1 (MQ=255)

                                               ccTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCg                                                                              >  2:340613/1‑140 (MQ=255)

                                                 tataAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGc                                                                            >  1:713224/1‑140 (MQ=255)

                                                 tataAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGc                                                                            >  1:823607/1‑140 (MQ=255)

                                                  ataAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCt                                                                           <  1:881954/140‑1 (MQ=255)

                                                  ataAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCt                                                                           >  1:837470/1‑140 (MQ=255)

                                                  ataAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCt                                                                           <  2:261309/140‑1 (MQ=255)

                                                    aaaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTaa                                                                         >  2:719847/1‑140 (MQ=255)

                                                    aaaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTaa                                                                         >  2:775446/1‑140 (MQ=255)

                                                    aaaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTaa                                                                         <  2:527486/140‑1 (MQ=255)

                                                    aaaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTaa                                                                         <  1:862526/140‑1 (MQ=255)

                                                    aaaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTaa                                                                         <  1:1064192/140‑1 (MQ=255)

                                                     aaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGAt                                                                                 <  2:408755/131‑1 (MQ=255)

                                                     aaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGAt                                                                                 >  1:898624/1‑131 (MQ=255)

                                                     aaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGAt                                                                                 >  1:408755/1‑131 (MQ=255)

                                                     aaGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGAt                                                                                 <  2:898624/131‑1 (MQ=255)

                                                          aaTACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCtt                                                                   >  1:955108/1‑140 (MQ=255)

                                                           aTACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCttt                                                                  >  2:946974/1‑140 (MQ=255)

                                                           aTACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCttt                                                                  >  1:312148/1‑140 (MQ=255)

                                                                   gCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTggg                                                          <  2:986801/140‑1 (MQ=255)

                                                                   gCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTggg                                                          <  1:340613/140‑1 (MQ=255)

                                                                   gCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTggg                                                          <  1:12042/140‑1 (MQ=255)

                                                                   gCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTggg                                                          <  1:708068/140‑1 (MQ=255)

                                                                        ggTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGtt                                                     <  1:775446/140‑1 (MQ=255)

                                                                         gTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTta                                                    >  2:892841/1‑140 (MQ=255)

                                                                         gTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTta                                                    >  2:463772/1‑140 (MQ=255)

                                                                          tGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTtat                                                   <  1:892841/140‑1 (MQ=255)

                                                                           gCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTtata                                                  <  1:989250/140‑1 (MQ=255)

                                                                              aaaaCACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACgg                                               >  2:738363/1‑140 (MQ=255)

                                                                                   acaTGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTg                                          >  2:171233/1‑140 (MQ=255)

                                                                                     aTGGCTTCGTTGTGGGCTGACTACGCCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGa                                        <  2:931161/140‑1 (MQ=255)

                                                                                     aTGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGa                                        >  1:1165321/1‑140 (MQ=255)

                                                                                     aTGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGa                                        <  2:713224/140‑1 (MQ=255)

                                                                                     aTGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGa                                        <  2:823607/140‑1 (MQ=255)

                                                                                      tGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGAt                                       >  1:820547/1‑140 (MQ=255)

                                                                                      tGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGAt                                       >  1:285438/1‑140 (MQ=255)

                                                                                          ttCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGt                                   <  2:955108/140‑1 (MQ=255)

                                                                                            cGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTa                                 <  1:946974/140‑1 (MQ=255)

                                                                                             gTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAg                                >  2:1101057/1‑140 (MQ=255)

                                                                                             gTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAg                                <  1:190611/140‑1 (MQ=255)

                                                                                              ttGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGt                               <  1:383775/140‑1 (MQ=255)

                                                                                              ttGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGt                               <  1:1004014/140‑1 (MQ=255)

                                                                                              ttGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGt                               <  2:740360/140‑1 (MQ=255)

                                                                                               tgtgGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTa                              <  1:872852/140‑1 (MQ=255)

                                                                                                   ggCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGtt                          <  2:114508/140‑1 (MQ=255)

                                                                                                      tGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGt                               >  2:144681/1‑132 (MQ=255)

                                                                                                      tGACTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGt                               <  1:144681/132‑1 (MQ=255)

                                                                                                        aCTACACCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGAt                     >  2:603719/1‑140 (MQ=255)

                                                                                                           acacCTTCTTTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTg                  >  1:743464/1‑140 (MQ=255)

                                                                                                                 tcttTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGa            <  1:310554/140‑1 (MQ=255)

                                                                                                                  cttTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAg           <  2:820547/140‑1 (MQ=255)

                                                                                                                  cttTGACGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAg           >  2:660333/1‑140 (MQ=255)

                                                                                                                       aCGGTCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGc      >  2:440656/1‑140 (MQ=255)

                                                                                                                           tCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCAtgg  >  1:511115/1‑140 (MQ=255)

                                                                                                                           tCCGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCAtgg  >  2:247179/1‑140 (MQ=255)

                                                                                                                            ccGCTTTCAGGCCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATGGTGTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCAtg   >  1:908818/1‑140 (MQ=255)

AGTCGGTTCTTATACTTACACCGATGCGGAATACACCACCGATACTACCTATAAAGGCAATACGCCTGCACAGGTGCCAAAACACATGGCTTCGTTGTGGGCTGACTACACCTTCTTTGACGGTCCGCTTTCAGGTCTGACGCTGGGCACCGGTGGTCGTTATACTGGCTCCAGTTATG‑‑GTGATCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGG  >  NC_000913/169334‑169596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`