Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,322,934	T→C	S357P (TCA→CCA)	atoC →	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,322,934	0	T	C	100.0%	208.1 / NA	58	S357P (TCA→CCA)	atoC	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/26);  total (32/26)

ATACTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGA  >  NC_000913/2322799‑2323061                                                                                                                                        |                                                                                                                                aTACTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATg                                                                                                                             >  1:704716/1‑140 (MQ=255)   aCTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGc                                                                                                                           <  2:382519/140‑1 (MQ=255)   aCTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGc                                                                                                                           <  1:439397/140‑1 (MQ=255)     tGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCg                                                                                                                         >  1:436471/1‑140 (MQ=255)      gccgccTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCgg                                                                                                                        >  1:492660/1‑140 (MQ=255)       ccgccTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCggg                                                                                                                       >  1:103623/1‑140 (MQ=255)       ccgccTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCggg                                                                                                                       >  2:829487/1‑140 (MQ=255)       ccgccTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCggg                                                                                                                       >  1:528391/1‑140 (MQ=255)           ctctGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCG‑GATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAata                                                                                                                  <  1:523763/140‑1 (MQ=255)               gcgcGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTc                                                                                                               >  2:902935/1‑140 (MQ=255)                  cgATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCgag                                                                                                            <  1:805920/140‑1 (MQ=255)                    aTCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGagag                                                                                                          <  2:559919/140‑1 (MQ=255)                    aTCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGagag                                                                                                          >  2:353795/1‑140 (MQ=255)                     tCGCCGGGCAGATATTTCCCTGTTAGCTAATCACTTTTTGCAACTATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGc                                                                                                         >  2:815858/1‑140 (MQ=255)                       gCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCtt                                                                                                       <  1:829487/140‑1 (MQ=255)                         cGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTc                                                                                                     <  1:1089985/140‑1 (MQ=255)                         cGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTc                                                                                                     <  2:479067/140‑1 (MQ=255)                            gAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCaa                                                                                                  <  2:528391/140‑1 (MQ=255)                               gATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGt                                                                                               >  2:114676/1‑140 (MQ=255)                                  atTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTAt                                                                                            <  2:436471/140‑1 (MQ=255)                                           ttAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACgcgc                                                                                   >  2:520430/1‑140 (MQ=255)                                               cTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCcgtc                                                                               <  2:64126/140‑1 (MQ=255)                                                  aTCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtg                                                                            >  2:106143/1‑140 (MQ=255)                                                   tCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtga                                                                           >  2:801591/1‑140 (MQ=255)                                                    cACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtgat                                                                          <  1:801591/140‑1 (MQ=255)                                                    cACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtgat                                                                          <  1:827405/140‑1 (MQ=255)                                                    cACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtgat                                                                          <  1:364848/140‑1 (MQ=255)                                                     aCTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGtgatg                                                                         >  1:7022/1‑140 (MQ=255)                                                        ttttGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAAt                                                                      <  1:321475/140‑1 (MQ=255)                                                         tttGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAAtt                                                                     >  2:720415/1‑140 (MQ=255)                                                               aaaaTTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGcc                                                               >  2:1160026/1‑140 (MQ=255)                                                                             agaATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCt                                                 >  2:195208/1‑140 (MQ=255)                                                                                aTCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAg                                              <  1:353795/140‑1 (MQ=255)                                                                                 tCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAgg                                             >  1:789584/1‑140 (MQ=255)                                                                                  cAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCTAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGa                                            >  1:501163/1‑140 (MQ=255)                                                                                  cAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGa                                            <  1:641793/140‑1 (MQ=255)                                                                                  cAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGa                                            <  2:103623/140‑1 (MQ=255)                                                                                  cAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGa                                            >  2:641793/1‑140 (MQ=255)                                                                                     cgcgATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCt                                         >  1:942518/1‑140 (MQ=255)                                                                                     cgcgATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCt                                         <  1:60191/140‑1 (MQ=255)                                                                                         atatTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCg                                     >  2:921288/1‑140 (MQ=255)                                                                                               tCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAg                               <  2:157806/140‑1 (MQ=255)                                                                                                    aTCGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCg                          >  1:264885/1‑140 (MQ=255)                                                                                                      cGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTc                        >  1:758914/1‑140 (MQ=255)                                                                                                      cGATCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTc                        >  1:701129/1‑140 (MQ=255)                                                                                                        aTCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAg                      >  2:75320/1‑140 (MQ=255)                                                                                                         tCCGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGc                     >  1:1015739/1‑140 (MQ=255)                                                                                                          ccGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGcc                    <  2:758914/140‑1 (MQ=255)                                                                                                           cGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCa                   <  2:701129/140‑1 (MQ=255)                                                                                                           cGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCa                   <  2:704716/140‑1 (MQ=255)                                                                                                           cGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCa                   <  1:720415/140‑1 (MQ=255)                                                                                                           cGATGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCa                   >  2:11087/1‑140 (MQ=255)                                                                                                             aTGGCAATGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGt                 <  1:195208/140‑1 (MQ=255)                                                                                                                   aTGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATTAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTaa           >  1:157831/1‑140 (MQ=255)                                                                                                                   aTGTCACTGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTaa           >  1:272241/1‑140 (MQ=255)                                                                                                                          tGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGc    >  1:71302/1‑140 (MQ=255)                                                                                                                          tGCTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGc    <  1:1150292/140‑1 (MQ=255)                                                                                                                            cTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGa  >  2:427371/1‑140 (MQ=255)                                                                                                                            cTTACCGCCTGGCCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGa  >  2:975052/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                ATACTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGA  >  NC_000913/2322799‑2323061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑