| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,329,419 | G→A | Q127* (CAA→TAA) | yfaQ ← | tandem DUF2300 domain‑containing protein YfaQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,329,419 | 0 | G | A | 97.9% | 153.9 / ‑4.3 | 48 | Q127* (CAA→TAA) | yfaQ | tandem DUF2300 domain‑containing protein YfaQ |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (27/20); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
AAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTC > NC_000913/2329283‑2329547 | aaGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTAccc > 1:273077/1‑140 (MQ=255) ttCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCcaca > 2:407406/1‑140 (MQ=255) ccTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCcacaca > 2:157541/1‑140 (MQ=255) ccTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCaacaca > 1:68338/1‑140 (MQ=255) gCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTgc < 2:273077/140‑1 (MQ=255) tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCa > 2:1078640/1‑140 (MQ=255) tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCa < 1:531338/140‑1 (MQ=255) cgcgGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCa > 1:536546/1‑140 (MQ=255) ggTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGcc < 2:517663/128‑1 (MQ=255) ggTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGcc > 1:517663/1‑128 (MQ=255) cGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgcg < 1:864315/140‑1 (MQ=255) cGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTgcg < 1:696498/140‑1 (MQ=255) gTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTgcgg > 2:282585/1‑140 (MQ=255) cTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCa < 2:719876/140‑1 (MQ=255) tAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCaa > 2:1187663/1‑140 (MQ=255) aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 2:299980/140‑1 (MQ=255) aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 1:608726/140‑1 (MQ=255) aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 1:1187663/140‑1 (MQ=255) aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 2:536546/140‑1 (MQ=255) tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTg > 1:430283/1‑125 (MQ=255) tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTg < 2:430283/125‑1 (MQ=255) tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATagag > 2:328647/1‑140 (MQ=255) cagcGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCcac < 2:570823/140‑1 (MQ=255) aTTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGaa > 2:1937/1‑140 (MQ=255) aCCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTAc < 1:1189853/140‑1 (MQ=255) aCCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAgcgc > 2:332571/1‑140 (MQ=255) aGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGTTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTa > 1:645101/1‑140 (MQ=255) ggTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTAt > 1:1183473/1‑140 (MQ=255) cTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATcgcgc > 2:314847/1‑140 (MQ=255) tCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATg > 2:456762/1‑140 (MQ=255) aGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct > 2:903613/1‑140 (MQ=255) gCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGctc > 1:945930/1‑140 (MQ=255) gCGTAGTCAGAGACGCTAACCACGCAGCTGCCTGACGCTGTTGCCAGCATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGctc > 2:509700/1‑140 (MQ=255) tAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTcc < 2:1183473/140‑1 (MQ=255) aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct < 2:1161114/135‑1 (MQ=255) aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct > 1:1161114/1‑135 (MQ=255) aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTccc < 1:314847/140‑1 (MQ=255) aCGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGa > 2:133614/1‑140 (MQ=255) cGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac > 1:288834/1‑140 (MQ=255) cGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac < 1:1110518/140‑1 (MQ=255) tAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGacaac > 1:114608/1‑140 (MQ=255) aaCCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGacaaca > 2:20228/1‑140 (MQ=255) aCCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCAACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGAcaacaa < 2:68338/140‑1 (MQ=255) cGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATaaa > 2:880088/1‑140 (MQ=255) gCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAAc < 1:407406/140‑1 (MQ=255) tGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac > 1:933920/1‑125 (MQ=255) tGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac < 2:933920/125‑1 (MQ=255) cGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGa > 2:707829/1‑140 (MQ=255) ttGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTc < 2:936120/140‑1 (MQ=255) | AAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTC > NC_000913/2329283‑2329547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |