Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,366,679	T→C	V257A (GTC→GCC)	arnB →	UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,366,679	0	T	C	100.0%	189.6 / NA	54	V257A (GTC→GCC)	arnB	UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/25);  total (29/25)

TTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCG  >  NC_000913/2366550‑2366814                                                                                                                                  |                                                                                                                                        ttCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTagag                                                                                                                               <  1:816405/140‑1 (MQ=255) ttCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTagag                                                                                                                               <  1:413817/140‑1 (MQ=255)    aCGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAAt                                                                                                                                    >  1:992370/1‑132 (MQ=255)    aCGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAAt                                                                                                                                    <  2:992370/132‑1 (MQ=255)         cTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCaa                                                                                                                       <  2:775325/140‑1 (MQ=255)                tCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGt                                                                                                                <  2:24191/140‑1 (MQ=255)                  gATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCg                                                                                                              >  1:78841/1‑140 (MQ=255)                      ccTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGTCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGgcgc                                                                                                          >  2:844885/1‑140 (MQ=255)                      ccTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGgcgc                                                                                                          >  2:548011/1‑140 (MQ=255)                              agacaAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGc                                                                                                  >  2:967508/1‑140 (MQ=255)                              agacaAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGc                                                                                                  <  1:703993/140‑1 (MQ=255)                                         gggCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATc                                                                                       >  1:855327/1‑140 (MQ=255)                                           gCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCag                                                                                     <  2:855327/140‑1 (MQ=255)                                               tGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAg                                                                                              >  1:750379/1‑127 (MQ=255)                                               tGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAg                                                                                              <  2:750379/127‑1 (MQ=255)                                               tGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCagcaag                                                                                 >  1:910934/1‑140 (MQ=255)                                                     gCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTgg                                                                           <  1:1181204/140‑1 (MQ=255)                                                      cAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGc                                                                          >  1:357837/1‑140 (MQ=255)                                                                          gggCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCAt                                                      >  1:157502/1‑140 (MQ=255)                                                                                      cAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCg                                          >  1:306207/1‑140 (MQ=255)                                                                                        aTCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGcc                                        >  1:564597/1‑140 (MQ=255)                                                                                        aTCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGcc                                        <  1:936884/140‑1 (MQ=255)                                                                                             aCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGcc                                   >  2:149838/1‑140 (MQ=255)                                                                                             aCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGcc                                   >  2:1055068/1‑140 (MQ=255)                                                                                             aCCGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGcc                                   >  2:301759/1‑140 (MQ=255)                                                                                              ccGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCg                                  >  2:131972/1‑140 (MQ=255)                                                                                              ccGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCg                                  >  1:627819/1‑140 (MQ=255)                                                                                              ccGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCg                                  >  2:1197182/1‑140 (MQ=255)                                                                                               cGATATTAACGCCGCGATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGc                                 <  2:952354/140‑1 (MQ=255)                                                                                                 atatTAACGCCGCGATTTCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCAGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGccc                               >  2:374122/1‑138 (MQ=255)                                                                                                           cgcgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCt                     <  1:194001/140‑1 (MQ=255)                                                                                                            gcgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                    >  1:1116083/1‑140 (MQ=255)                                                                                                            gcgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                    >  2:486992/1‑140 (MQ=255)                                                                                                            gcgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                    >  2:876620/1‑140 (MQ=255)                                                                                                            gcgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTg                    >  2:1202404/1‑140 (MQ=255)                                                                                                             cgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTgg                   >  2:1059762/1‑140 (MQ=255)                                                                                                             cgATTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTgg                   >  2:494285/1‑140 (MQ=255)                                                                                                               aTTGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACCCCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCa                 <  2:1787/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  1:1202404/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  2:157502/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  2:160216/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  2:1023109/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  1:149838/140‑1 (MQ=255)                                                                                                                ttGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCAt                <  1:301759/140‑1 (MQ=255)                                                                                                                 tGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATc               <  1:876620/140‑1 (MQ=255)                                                                                                                 tGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATc               <  1:131972/140‑1 (MQ=255)                                                                                                                 tGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATc               <  1:1197182/140‑1 (MQ=255)                                                                                                                 tGCCCTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATc               <  2:627819/140‑1 (MQ=255)                                                                                                                     cTGACACAGTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGtt           >  1:447378/1‑140 (MQ=255)                                                                                                                             gTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt              >  1:106581/1‑129 (MQ=255)                                                                                                                             gTTAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt              <  2:106581/129‑1 (MQ=255)                                                                                                                              ttAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg  >  1:542958/1‑140 (MQ=255)                                                                                                                              ttAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCg  >  1:898741/1‑140 (MQ=255)                                                                                                                              ttAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTAAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt              >  2:802448/1‑128 (MQ=255)                                                                                                                              ttAGCCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTAAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCt              <  1:802448/128‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTATAAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAGCACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTCCCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATTATTCG  >  NC_000913/2366550‑2366814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑