BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,575	A→G	G165G (GGT→GGC)	yaeI ←	phosphodiesterase YaeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,575	0	A	G	98.7%	244.0 / ‑4.8	76	G165G (GGT→GGC)	yaeI	phosphodiesterase YaeI
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (34/41); total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

TGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCACCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTGTA  >  NC_000913/184438‑184692

tGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGcc                                                                                                                     >  1:956931/1‑140 (MQ=255)

tGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGcc                                                                                                                     >  2:1100992/1‑140 (MQ=255)

  aGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCACCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGGGGCGGTTTGCATTGTCCCGCCCCTAAGTTGCCAg                                                                                                                   >  2:582123/1‑140 (MQ=255)

   gTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGt                                                                                                                  <  2:692678/140‑1 (MQ=255)

   gTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGt                                                                                                                  <  2:938897/140‑1 (MQ=255)

   gTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGt                                                                                                                  >  2:427610/1‑140 (MQ=255)

    tATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTg                                                                                                                 >  1:246985/1‑140 (MQ=255)

        gCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTa                                                                                                             >  2:55850/1‑140 (MQ=255)

        gCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTa                                                                                                             <  2:83652/140‑1 (MQ=255)

        gCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTa                                                                                                             >  2:383669/1‑140 (MQ=255)

            cacaGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAg                                                                                                         >  2:1150225/1‑140 (MQ=255)

            cacaGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAg                                                                                                         >  2:1194813/1‑140 (MQ=255)

            cacaGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAg                                                                                                         >  2:404651/1‑140 (MQ=255)

            cacaGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAg                                                                                                         >  2:494630/1‑140 (MQ=255)

             acaGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGGCCGGCCCATAAGTCACCAGTGCCTACCAGt                                                                                                        <  1:700294/140‑1 (MQ=255)

                 cATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGa                                                                                                    >  1:864312/1‑140 (MQ=255)

                  aTCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCAGAAGTCGCCAGTGCCTACCAGTTCGaa                                                                                                   >  1:850886/1‑140 (MQ=255)

                   tCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAAt                                                                                                  <  1:1034065/140‑1 (MQ=255)

                       aTCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGa                                                                                                    >  2:637863/1‑134 (MQ=255)

                       aTCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGa                                                                                                    <  1:637863/134‑1 (MQ=255)

                       aTCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGGTTGCATTGTCCGGCCCATAAGTCCCCAGTGCCTAACAGGTCGAATTGcc                                                                                              >  2:748292/1‑140 (MQ=255)

                           cAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCCTAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGtt                                                                                          >  2:715855/1‑140 (MQ=255)

                            aGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTc                                                                                         >  1:171344/1‑140 (MQ=255)

                            aGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGGTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTc                                                                                         >  1:75138/1‑140 (MQ=255)

                             gggTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCg                                                                                        <  2:171344/140‑1 (MQ=255)

                                  catcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTg                                                                                   <  2:956931/140‑1 (MQ=255)

                                  catcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTg                                                                                   <  1:317057/140‑1 (MQ=255)

                                  catcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTg                                                                                   <  1:910632/140‑1 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGt                                                                                    >  1:52477/1‑138 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGt                                                                                    <  2:52477/138‑1 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTgg                                                                                  >  1:52706/1‑140 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTgg                                                                                  >  2:240205/1‑140 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTgg                                                                                  >  1:264437/1‑140 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTgg                                                                                  >  1:852210/1‑140 (MQ=255)

                                   atcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTgg                                                                                  >  1:628445/1‑140 (MQ=255)

                                    tcaCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGc                                                                                 <  1:494630/140‑1 (MQ=255)

                                      aCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGa                                                                               <  2:349654/140‑1 (MQ=255)

                                      aCGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGa                                                                               <  1:654015/140‑1 (MQ=255)

                                          aTGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCAc                                                                           >  2:1102827/1‑140 (MQ=255)

                                               ttcttTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAg                                                                      <  2:628445/140‑1 (MQ=255)

                                                tcttTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGc                                                                     <  2:52706/140‑1 (MQ=255)

                                                    tGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGa                                                                               >  1:278311/1‑126 (MQ=255)

                                                    tGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGa                                                                               <  2:278311/126‑1 (MQ=255)

                                                    tGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTgg                                                                 <  2:264437/140‑1 (MQ=255)

                                                    tGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTgg                                                                 >  2:312119/1‑140 (MQ=255)

                                                      cTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGtt                                                               <  1:427610/140‑1 (MQ=255)

                                                       tGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAg                                                                      <  1:354588/132‑1 (MQ=255)

                                                       tGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAg                                                                      >  2:354588/1‑132 (MQ=255)

                                                       tGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTa                                                              <  2:497185/140‑1 (MQ=255)

                                                       tGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTa                                                              <  2:864312/140‑1 (MQ=255)

                                                         tCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTaaa                                                            <  2:850886/140‑1 (MQ=255)

                                                         tCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTaaa                                                            <  2:852210/140‑1 (MQ=255)

                                                          cGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAaac                                                           <  1:1194813/140‑1 (MQ=255)

                                                            ggATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAaacaa                                                         <  1:355952/140‑1 (MQ=255)

                                                                tATGCGCCAGCCCCAGCCTTGGCAGATTGGCTTCCCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTCCCTACCAGTTCCACTCGCCTGTTCGGCGTGGCGATACCCGTAGCCTGGTTAAACAACAcc                                                     <  2:513438/140‑1 (MQ=255)

                                                                   gcgcCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTg                                                  >  1:899105/1‑140 (MQ=255)

                                                                     gcCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGAt                                                >  2:193908/1‑140 (MQ=255)

                                                                          cACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCg                                           <  1:748292/140‑1 (MQ=255)

                                                                           aCCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGc                                          <  2:61238/140‑1 (MQ=255)

                                                                            ccAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCt                                         <  1:1100992/140‑1 (MQ=255)

                                                                              aGTCTTGGCAGATTGGC‑TCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGAt                                      >  1:817383/1‑140 (MQ=255)

                                                                               gTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGAt                                      >  1:1077797/1‑140 (MQ=255)

                                                                                  ttGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTc                                   <  1:240205/140‑1 (MQ=255)

                                                                                    ggCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCCTTGTGCGGCCCATAATGCGCCAGTGCCTAACAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTTAACAACACCGTGATGCCCGCTGATTTTaa                                 >  2:799158/1‑140 (MQ=255)

                                                                                    ggCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCaa                                 >  1:1199544/1‑140 (MQ=255)

                                                                                       aGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGt                              <  1:147651/140‑1 (MQ=255)

                                                                                       aGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGt                              <  2:916634/140‑1 (MQ=255)

                                                                                        gATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGtc                             >  1:1205013/1‑140 (MQ=255)

                                                                                        gATTGGC‑TCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGtct                            <  2:817383/140‑1 (MQ=255)

                                                                                          ttGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGtctc                           <  1:1150225/140‑1 (MQ=255)

                                                                                          ttGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGtctc                           <  1:404651/140‑1 (MQ=255)

                                                                                            ggCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGc                         <  2:1007159/140‑1 (MQ=255)

                                                                                            ggCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGc                         <  1:193908/140‑1 (MQ=255)

                                                                                                   cTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCaa                                 <  1:968407/125‑1 (MQ=255)

                                                                                                   cTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCaa                                 >  2:968407/1‑125 (MQ=255)

                                                                                                    tGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAg                 <  1:383669/140‑1 (MQ=255)

                                                                                                            ggCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGAttttt         >  1:667613/1‑140 (MQ=255)

                                                                                                             gCGGTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGAtttttt        <  1:582123/140‑1 (MQ=255)

                                                                                                                gTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCt     >  2:391201/1‑140 (MQ=255)

                                                                                                                gTTTGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCt     >  1:558033/1‑140 (MQ=255)

                                                                                                                gTTTGCATTGTCCGGCCCATAAGGCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCt     >  2:86517/1‑140 (MQ=255)

                                                                                                                 tttGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTg    <  2:558033/140‑1 (MQ=255)

                                                                                                                   tGCATTGTCCGGCCCATAAGTCGCCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTGTa  <  1:55850/140‑1 (MQ=255)

TGAGTATGGCCGCACAGCATCAGATCCCAGGGTTCATCACGCATGACTTCTTTGCTGTCGGGATTATGCGCCAGCACCAGTCTTGGCAGATTGGCTTCGCTGGCGGGAGGCGGTTTGCATTGTCCGGCCCATAAGTCACCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTGTA  >  NC_000913/184438‑184692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`