Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,339	G→A	P104L (CCC→CTC)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,339	0	G	A	100.0%	165.5 / NA	48	P104L (CCC→CTC)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/19);  total (29/19)

AACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCG  >  NC_000913/2436202‑2436473                                                                                                                                          |                                                                                                                                       aaCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGt                                                                                                                                      >  1:488495/1‑140 (MQ=255)  aCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGtt                                                                                                                                     >  1:879309/1‑140 (MQ=255)  aCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGtt                                                                                                                                     >  1:266921/1‑140 (MQ=255)    cGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   >  1:689637/1‑140 (MQ=255)      cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 <  2:488495/140‑1 (MQ=255)      cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 >  2:382569/1‑140 (MQ=255)      cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 >  2:200140/1‑140 (MQ=255)        cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   >  1:95395/1‑136 (MQ=255)        cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   <  2:95395/136‑1 (MQ=255)         tGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGc                                                                                                                              >  1:663577/1‑140 (MQ=255)                 tAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATc                                                                                                                      <  2:15818/140‑1 (MQ=255)                 tAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATc                                                                                                                      <  1:200140/140‑1 (MQ=255)                    cGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCAc                                                                                                                   <  2:590363/140‑1 (MQ=255)                     ggTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCAct                                                                                                                  >  1:175070/1‑140 (MQ=255)                         tCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCActgctg                                                                                                              >  2:121882/1‑140 (MQ=255)                         tCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCActgctg                                                                                                              >  2:488136/1‑140 (MQ=255)                          cAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGt                                                                                                             >  1:1028832/1‑140 (MQ=255)                             tGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGa                                                                                                          >  1:266729/1‑140 (MQ=255)                                 gccAGCAGCTGGCT‑GTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCAcc                                                                                                     >  2:32017/1‑140 (MQ=255)                                    agcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAg                                                                                                   <  2:1028832/140‑1 (MQ=255)                                     gcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAgg                                                                                                  <  2:266729/140‑1 (MQ=255)                                        gctggctgGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCaa                                                                                               <  1:179858/140‑1 (MQ=255)                                                     aCTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCg                                                                                  <  1:488136/140‑1 (MQ=255)                                                          cTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGcttct                                                                             >  1:912901/1‑140 (MQ=255)                                                          cTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGcttct                                                                             <  2:879309/140‑1 (MQ=255)                                                              tACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa                                                                         >  1:653828/1‑140 (MQ=255)                                                                 ggCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAAccc                                                                      >  1:1128400/1‑140 (MQ=255)                                                                   cGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAg                                                                    >  2:764628/1‑140 (MQ=255)                                                                    gATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAgg                                                                   <  2:663577/140‑1 (MQ=255)                                                                     aTGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGc                                                                  >  2:354382/1‑140 (MQ=255)                                                                            tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCAGCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           <  1:455225/140‑1 (MQ=255)                                                                            tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           >  1:644599/1‑140 (MQ=255)                                                                            tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           <  2:912901/140‑1 (MQ=255)                                                                                             gTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGAc                                          >  2:219034/1‑140 (MQ=255)                                                                                                       aCGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGcc                                <  1:32017/140‑1 (MQ=255)                                                                                                        cGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCa                               >  2:799194/1‑140 (MQ=255)                                                                                                        cGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCa                               >  1:345517/1‑140 (MQ=255)                                                                                                         gggTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAg                              <  1:382569/140‑1 (MQ=255)                                                                                                            tttACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGTCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTg                           >  2:567202/1‑140 (MQ=255)                                                                                                                          caccaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGGGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGt             >  1:1048343/1‑140 (MQ=255)                                                                                                                           accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg              >  2:1038046/1‑138 (MQ=255)                                                                                                                           accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg              <  1:1038046/138‑1 (MQ=255)                                                                                                                           accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTc            >  2:344244/1‑140 (MQ=255)                                                                                                                           accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTc            >  2:825307/1‑140 (MQ=255)                                                                                                                            ccaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCg           <  2:266921/140‑1 (MQ=255)                                                                                                                                     cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  <  1:825307/140‑1 (MQ=255)                                                                                                                                     cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  >  1:669248/1‑140 (MQ=255)                                                                                                                                     cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  <  1:219034/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCG  >  NC_000913/2436202‑2436473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑