Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,102	A→G	G686G (GGT→GGC)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,102	0	A	G	100.0%	295.3 / NA	84	G686G (GGT→GGC)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/43);  total (41/43)

TTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGC  >  NC_000913/2456966‑2457238                                                                                                                                         |                                                                                                                                         ttGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCa                                                                                                                                       <  2:719725/140‑1 (MQ=255)   gACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATa                                                                                                                                     <  2:258488/140‑1 (MQ=255)    aCCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATAc                                                                                                                                    >  1:255311/1‑140 (MQ=255)      cAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  <  2:1136162/140‑1 (MQ=255)        acacTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCg                                                                                                                                >  2:673943/1‑140 (MQ=255)            tCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGc                                                                                                                            >  1:134930/1‑140 (MQ=255)              cGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCaa                                                                                                                          >  1:371534/1‑140 (MQ=255)                ccATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGt                                                                                                                        >  2:378595/1‑140 (MQ=255)                  aTTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCg                                                                                                                      <  1:1073140/140‑1 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  <  2:784431/127‑1 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  >  1:323996/1‑127 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  <  2:693887/127‑1 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  >  1:784431/1‑127 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  <  2:323996/127‑1 (MQ=255)                   ttCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTg                                                                                                                                  >  1:693887/1‑127 (MQ=255)                        cgcgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCat                                                                                                                >  2:850499/1‑140 (MQ=255)                         gcgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCatt                                                                                                               <  2:286893/140‑1 (MQ=255)                             aTTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTg                                                                                                           >  1:804319/1‑140 (MQ=255)                             aTTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTg                                                                                                           >  1:161398/1‑140 (MQ=255)                               tCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCa                                                                                                         <  1:850499/140‑1 (MQ=255)                                       cTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTc                                                                                                 >  2:486141/1‑140 (MQ=255)                                       cTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTc                                                                                                 >  2:579937/1‑140 (MQ=255)                                         ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCa                                                                                                                 <  2:734834/122‑1 (MQ=255)                                         ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCa                                                                                                                 >  1:734834/1‑122 (MQ=255)                                          tGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGcc                                                                                              <  1:486141/140‑1 (MQ=255)                                          tGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGcc                                                                                              >  1:448218/1‑140 (MQ=255)                                           gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGccc                                                                                             >  1:963753/1‑140 (MQ=255)                                           gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGccc                                                                                             >  2:958303/1‑140 (MQ=255)                                            aCCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCTACCACTTCGCCcg                                                                                            >  1:733572/1‑140 (MQ=255)                                            aCCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCcg                                                                                            >  2:567531/1‑140 (MQ=255)                                            aCCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCcg                                                                                            >  2:315698/1‑140 (MQ=255)                                            aCCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCcg                                                                                            >  2:824041/1‑140 (MQ=255)                                                  ttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCga                                                                                      <  1:579937/140‑1 (MQ=255)                                                   tATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                     <  2:482929/140‑1 (MQ=255)                                                   tATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                     <  2:868862/140‑1 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                     >  1:541161/1‑132 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                     <  2:541161/132‑1 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                             >  1:716448/1‑140 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                             >  2:817282/1‑140 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                             >  2:411896/1‑140 (MQ=255)                                                           gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                             >  2:975219/1‑140 (MQ=255)                                                            tCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGat                                                                            >  1:988858/1‑140 (MQ=255)                                                            tCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGat                                                                            >  1:1085181/1‑140 (MQ=255)                                                             cagttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGata                                                                           <  1:975219/140‑1 (MQ=255)                                                                ttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAg                                                                        <  1:145900/140‑1 (MQ=255)                                                                 tgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGc                                                                       >  1:1198133/1‑140 (MQ=255)                                                                      ttgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAAc                                                                  <  2:1152613/140‑1 (MQ=255)                                                                       tgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACg                                                                 <  1:315698/140‑1 (MQ=255)                                                                         ttttCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGt                                                               >  1:1004178/1‑140 (MQ=255)                                                                          tttCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTc                                                              >  2:705807/1‑140 (MQ=255)                                                                          tttCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTc                                                              >  2:1124422/1‑140 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGGGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  1:655333/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  2:255311/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  1:747972/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  1:1124422/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  1:705807/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  2:716448/140‑1 (MQ=255)                                                                                                 cgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  1:580643/140‑1 (MQ=255)                                                                                                        ctcGACCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCaa                                >  1:221091/1‑140 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGGCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAc                            <  2:1085181/140‑1 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       >  1:655273/1‑129 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                       <  2:655273/129‑1 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAc                            >  1:387926/1‑140 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAc                            <  2:988858/140‑1 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAc                            >  2:400719/1‑140 (MQ=255)                                                                                                            aCCAAACGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAc                            <  2:387926/140‑1 (MQ=255)                                                                                                                aaCGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACcgcg                        >  2:617289/1‑140 (MQ=255)                                                                                                                 aCGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAAc                                         <  2:922259/122‑1 (MQ=255)                                                                                                                 aCGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAAc                                         >  1:922259/1‑122 (MQ=255)                                                                                                                 aCGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCgcgc                       <  2:134930/140‑1 (MQ=255)                                                                                                                  cGCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGcc                      >  1:1211856/1‑140 (MQ=255)                                                                                                                   gCTCGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCa                     >  2:595542/1‑140 (MQ=255)                                                                                                                      cGCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAata                  >  1:841673/1‑140 (MQ=255)                                                                                                                       gCAAGGGGTAAAACGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAatat                 <  1:673943/140‑1 (MQ=255)                                                                                                                                 aaaCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacg       <  1:400719/140‑1 (MQ=255)                                                                                                                                 aaaCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTACAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacg       <  2:968840/140‑1 (MQ=255)                                                                                                                                   aCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca     <  1:1201561/140‑1 (MQ=255)                                                                                                                                   aCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca     <  1:49691/140‑1 (MQ=255)                                                                                                                                   aCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca     <  2:221091/140‑1 (MQ=255)                                                                                                                                   aCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca     <  2:161398/140‑1 (MQ=255)                                                                                                                                   aCGGGAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgca     <  1:411896/140‑1 (MQ=255)                                                                                                                                     gggAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTcacgcacg   <  1:817282/140‑1 (MQ=255)                                                                                                                                      ggAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCacgcacgc  <  2:371534/140‑1 (MQ=255)                                                                                                                                      ggAGCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCacgcacgc  <  1:187053/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGC  >  NC_000913/2456966‑2457238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑