Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,025	T→C	L384L (TTA→TTG)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,025	0	T	C	100.0%	280.8 / NA	79	L384L (TTA→TTG)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/36);  total (43/36)

ATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTAAATGTCACTAAC  >  NC_000913/2480889‑2481160                                                                                                                                         |                                                                                                                                        aTTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCaaa                                                                                                                                      <  2:99524/140‑1 (MQ=255)    cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAg                                                                                                                                   >  2:743734/1‑140 (MQ=255)    cgcgTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAg                                                                                                                                   <  2:289957/140‑1 (MQ=255)          gtcCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAg                                                                                                                             >  1:4320/1‑140 (MQ=255)                acaGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACg                                                                                                                       >  2:1136581/1‑140 (MQ=255)                    cGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGa                                                                                                                   >  2:936568/1‑140 (MQ=255)                      tCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGACGAAt                                                                                                                 >  2:244493/1‑140 (MQ=255)                       cATTGTCAACGACGTATCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATc                                                                                                                >  1:220915/1‑140 (MQ=255)                       cATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATc                                                                                                                <  2:468868/140‑1 (MQ=255)                            tCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGa                                                                                                           <  1:743734/140‑1 (MQ=255)                               acgacgTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGaaaa                                                                                                        >  2:1043061/1‑140 (MQ=255)                                   cgTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACt                                                                                                    >  2:867395/1‑140 (MQ=255)                                    gTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTg                                                                                                   <  1:1068001/140‑1 (MQ=255)                                      aCCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCg                                                                                                 >  1:747762/1‑140 (MQ=255)                                      aCCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCg                                                                                                 >  1:360168/1‑140 (MQ=255)                                      aCCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCg                                                                                                 >  1:363652/1‑140 (MQ=255)                                      aCCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCg                                                                                                 >  2:156597/1‑140 (MQ=255)                                            tGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCaaaa                                                                                           <  1:156597/140‑1 (MQ=255)                                             gATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAat                                                                                          <  1:67327/140‑1 (MQ=255)                                                ccTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat                                                                                       <  1:867395/140‑1 (MQ=255)                                                ccTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat                                                                                       >  1:991086/1‑140 (MQ=255)                                                ccTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat                                                                                       <  2:737878/140‑1 (MQ=255)                                                ccTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatga                                                                                        <  1:734511/139‑1 (MQ=255)                                                ccTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatga                                                                                        >  2:734511/1‑139 (MQ=255)                                                    gcAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCt                                                                                   >  2:430756/2‑140 (MQ=255)                                                    aCAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCt                                                                                   >  1:1031882/1‑140 (MQ=255)                                                    aCAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCt                                                                                   <  2:266806/140‑1 (MQ=255)                                                    aCAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCt                                                                                   >  2:512860/1‑140 (MQ=255)                                                     cAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat                                                                                       <  2:1204911/135‑1 (MQ=255)                                                     cAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAatgat                                                                                       >  1:1204911/1‑135 (MQ=255)                                                      aaGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGt                                                                                 >  2:237541/1‑140 (MQ=255)                                                        ggTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGTGGAAAAATGATGCCTGTaa                                                                               >  2:638176/1‑140 (MQ=255)                                                         gTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAACCTGAAAACGAAATCGTTGTTACGGGCAAAAAGAAACAGGCAACGGAGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTaaa                                                                              >  2:308955/1‑140 (MQ=255)                                                          tACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTaaaa                                                                             >  2:623969/1‑140 (MQ=255)                                                          tACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTaaaa                                                                             >  2:273358/1‑140 (MQ=255)                                                              aaaaaaaTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAaatcaa                                                                         >  2:327081/1‑140 (MQ=255)                                                                 aaaaTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCg                                                                      <  1:936568/140‑1 (MQ=255)                                                                 aaaaTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCg                                                                      <  2:213325/140‑1 (MQ=255)                                                                  aaaTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGt                                                                     <  1:306333/140‑1 (MQ=255)                                                                   aaTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGtt                                                                    >  2:496876/1‑140 (MQ=255)                                                                    aTTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTg                                                                   >  1:1042572/1‑140 (MQ=255)                                                                      tACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGc                                                                 <  2:523661/140‑1 (MQ=255)                                                                                 gAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTcac                                                      >  2:865290/1‑140 (MQ=255)                                                                                  aGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTcaca                                                     >  1:551275/1‑140 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    <  2:175698/140‑1 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    >  1:287498/1‑140 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    <  2:551275/140‑1 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    <  1:496876/140‑1 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    >  2:292607/1‑140 (MQ=255)                                                                                   ggCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATACCTGTAAAATCAATCGTTGGCATAAATGTCACAg                                                    >  2:763577/1‑140 (MQ=255)                                                                                       ttGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACg                                                <  1:623969/140‑1 (MQ=255)                                                                                        tGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATTATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGc                                               >  2:1119785/1‑140 (MQ=255)                                                                                        tGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGc                                               <  2:835175/140‑1 (MQ=255)                                                                                         ggCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGcc                                              >  1:863917/1‑140 (MQ=255)                                                                                           cAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAg                                            <  1:512860/140‑1 (MQ=255)                                                                                            aaaTTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCGTAAATGTCACAGAACGCCAgc                                           <  1:1136581/140‑2 (MQ=255)                                                                                               tttATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAAt                                        >  2:927209/1‑140 (MQ=255)                                                                                                  attatCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGc                                     <  1:865290/140‑1 (MQ=255)                                                                                                  attatCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGc                                     <  1:1043061/140‑1 (MQ=255)                                                                                                   ttatCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCa                                    <  1:850663/140‑1 (MQ=255)                                                                                                        tGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCg                               >  2:648859/1‑140 (MQ=255)                                                                                                         ggCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGc                              >  2:2226/1‑140 (MQ=255)                                                                                                         ggCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGc                              <  2:978708/140‑1 (MQ=255)                                                                                                          gCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCa                             >  1:105666/1‑140 (MQ=255)                                                                                                          gCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCa                             >  2:301291/1‑140 (MQ=255)                                                                                                          gCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCa                             <  1:648571/140‑1 (MQ=255)                                                                                                          gCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCa                             <  2:1042572/140‑1 (MQ=255)                                                                                                          gCAAGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCa                             <  2:1031882/140‑1 (MQ=255)                                                                                                             aGCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATAc                          >  1:607878/1‑140 (MQ=255)                                                                                                              gCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACa                         >  2:866320/1‑140 (MQ=255)                                                                                                              gCCTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACa                         >  2:111832/1‑140 (MQ=255)                                                                                                                cTGAAAACGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATc                       >  2:1175238/1‑140 (MQ=255)                                                                                                                    aaaCGAAATCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCaaaa                   <  2:79523/140‑1 (MQ=255)                                                                                                                         aaaTCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTa              <  2:287498/140‑1 (MQ=255)                                                                                                                         aaaTCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTa              >  1:186826/1‑140 (MQ=255)                                                                                                                          aaTCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTaa             <  1:608515/140‑1 (MQ=255)                                                                                                                          aaTCGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTaa             <  1:927209/140‑1 (MQ=255)                                                                                                                             cGTTGTTAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTAAATg          >  1:51165/1‑140 (MQ=255)                                                                                                                                tgttAAGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGTGGAAAAATGATGCCTGTAAAATTAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAATTAAATGTCa       <  1:638176/140‑1 (MQ=255)                                                                                                                                     aGGGCAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTAAATGTCACTaac  <  2:778070/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGGGTAAAAAGAAACAGGCAACGGCGAATCCCTGAAAAAACTGCGGCAAAATGATGCCTGTAAAATCAATCGTTGGCATAAATGTCACAGAACGCCAGTAATAGCAAACCGCATACATCAAAAAACTAAATGTCACTAAC  >  NC_000913/2480889‑2481160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑