Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,545,766	A→G	intergenic (‑157/‑7)	murR ← / → murQ	DNA‑binding transcriptional dual regulator MurR/N‑acetylmuramic acid 6‑phosphate etherase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,545,766	0	A	G	100.0%	175.3 / NA	51	intergenic (‑157/‑7)	murR/murQ	DNA‑binding transcriptional dual regulator MurR/N‑acetylmuramic acid 6‑phosphate etherase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/20);  total (31/20)

GCAGCATAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCG  >  NC_000913/2545629‑2545902                                                                                                                                          |                                                                                                                                         gcagcaTAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTcgcc                                                                                                                                        >  2:667605/1‑140 (MQ=255) gcagcaTAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTcgcc                                                                                                                                        >  2:132023/1‑140 (MQ=255) gcagcaTAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTcgcc                                                                                                                                        >  1:32018/1‑140 (MQ=255)       tAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGAt                                                                                                                                  >  2:164807/1‑140 (MQ=255)                 ttAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGaa                                                                                                                        <  2:457443/140‑1 (MQ=255)                              aCGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTg                                                                                                           <  2:364204/140‑1 (MQ=255)                                gCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGaa                                                                                                         <  1:132023/140‑1 (MQ=255)                                gCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGaa                                                                                                         >  1:725420/1‑140 (MQ=255)                                            ctGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACAcc                                                                                             >  2:534883/1‑140 (MQ=255)                                             tGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCg                                                                                            >  1:977074/1‑140 (MQ=255)                                              gCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGc                                                                                           >  1:242469/1‑140 (MQ=255)                                               cGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGcc                                                                                          <  2:958186/140‑1 (MQ=255)                                               cGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGcc                                                                                          <  2:242469/140‑1 (MQ=255)                                                 aGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTc                                                                                        >  2:600348/1‑140 (MQ=255)                                                     tAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCt                                                                                    <  2:32018/140‑1 (MQ=255)                                                       gACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGa                                                                                  >  2:1149760/1‑140 (MQ=255)                                                       gACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGa                                                                                  >  2:1052336/1‑140 (MQ=255)                                                          tCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAAt                                                                               <  1:1052336/140‑1 (MQ=255)                                                            aaTGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTg                                                                             >  2:884649/1‑140 (MQ=255)                                                             aTGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATTATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGa                                                                            >  2:774772/1‑140 (MQ=255)                                                                      ttAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATc                                                                   >  2:191077/1‑140 (MQ=255)                                                                              gTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTgg                                                           <  1:1149760/140‑1 (MQ=255)                                                                                cAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGaa                                                         <  2:725420/140‑1 (MQ=255)                                                                                  aTTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAAt                                                       >  1:480718/1‑140 (MQ=255)                                                                                   ttCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTAGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAAtg                                                      >  1:919742/1‑140 (MQ=255)                                                                                    tCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAAtgt                                                     <  1:534883/140‑1 (MQ=255)                                                                                     ggggATATATGATTCCATACATCTGAAATTATTTGATCGTAAAAAGTAAGGTGGCCACCGATGCAATTTGAAAAGGTGATTCCTGAGGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAAtgtg                                                    <  2:919742/139‑1 (MQ=255)                                                                                      ggtatatatGATTCCATACATCTGCAATTACTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGc                                                   <  2:744598/137‑1 (MQ=255)                                                                                         atatatGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCgg                                                >  2:958087/1‑140 (MQ=255)                                                                                         atatatGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCgg                                                >  2:136877/1‑140 (MQ=255)                                                                                                    cATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGa                                     >  2:972527/1‑140 (MQ=255)                                                                                                    cATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGa                                     >  1:344662/1‑140 (MQ=255)                                                                                                     aTACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATTATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                    <  1:774772/140‑1 (MQ=255)                                                                                                     aTACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGAt                                    <  1:972527/140‑1 (MQ=255)                                                                                                      tACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATg                                   >  2:946432/1‑140 (MQ=255)                                                                                                      tACATCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATg                                   >  1:204026/1‑140 (MQ=255)                                                                                                      tACATCTGCAATTATTTGATCGTAAATAGGAAGGTCGCCACCGCTGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACAGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGCGCCGGATTATCAACGATg                                   >  2:868153/1‑140 (MQ=255)                                                                                                         aTCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTTACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAg                                >  1:394406/1‑140 (MQ=255)                                                                                                         aTCTGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAg                                >  1:830654/1‑140 (MQ=255)                                                                                                            tGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATa                             >  1:232626/1‑140 (MQ=255)                                                                                                            tGCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATa                             >  2:689057/1‑140 (MQ=255)                                                                                                             gCAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATaa                            <  1:946432/140‑1 (MQ=255)                                                                                                              cAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATaaa                           >  1:1057967/1‑140 (MQ=255)                                                                                                              cAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATaaa                           >  2:537305/1‑140 (MQ=255)                                                                                                              cAATTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATaaa                           >  2:288790/1‑140 (MQ=255)                                                                                                               aaTTATTTGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATaaaa                          >  2:1175117/1‑140 (MQ=255)                                                                                                                    tttGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTa                     <  2:344662/140‑1 (MQ=255)                                                                                                                     ttGATCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTAc                    >  2:960599/1‑140 (MQ=255)                                                                                                                        aTCGTAAATAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGc                 <  2:945094/140‑1 (MQ=255)                                                                                                                               aTAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGt          <  1:689057/140‑1 (MQ=255)                                                                                                                                tAGTAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGtt         <  1:960599/140‑1 (MQ=255)                                                                                                                                   tAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAg      >  1:676527/1‑140 (MQ=255)                                                                                                                                   tAAGGTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAg      >  1:1150920/1‑140 (MQ=255)                                                                                                                                      ggTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAgcgc   >  1:654028/1‑140 (MQ=255)                                                                                                                                       gTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg  <  2:830654/140‑1 (MQ=255)                                                                                                                                       gTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg  <  1:863529/140‑1 (MQ=255)                                                                                                                                       gTCGCCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGcgcg  <  1:191077/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCAGCATAAATTCCGCTTAAGTTCTTATGACGCTCTTCACACTCTGCGAGTGTAGACTCAATGATTCCTTTAAGCCTGTCAATTCGGGATATATGATTCCATACATCTGCAATTATTTGATCGTAAATAGTAAGGTCACCACCGATGCAATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCG  >  NC_000913/2545629‑2545902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑