Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,554,937	A→G	G82G (GGT→GGC)	eutR ←	putative AraC‑type transcriptional regulator EutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,554,937	0	A	G	100.0%	149.9 / NA	43	G82G (GGT→GGC)	eutR	putative AraC‑type transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

GTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTG  >  NC_000913/2554803‑2555073                                                                                                                                       |                                                                                                                                         gTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGgt                                                                                                                                     >  2:792616/1‑140 (MQ=255)      accaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTAtt                                                                                                                                <  1:792616/140‑1 (MQ=255)      accaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTAtt                                                                                                                                <  1:421734/140‑1 (MQ=255)       ccaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTc                                                                                                                               >  1:257206/1‑140 (MQ=255)        caGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCa                                                                                                                              >  1:582104/1‑140 (MQ=255)             cGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGaaa                                                                                                                         <  2:257206/140‑1 (MQ=255)                gTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAAc                                                                                                                      <  2:119482/140‑1 (MQ=255)                         tCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATatc                                                                                                             <  1:578131/140‑1 (MQ=255)                          ccGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATatca                                                                                                            <  2:627428/140‑1 (MQ=255)                                          ttGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATGTCATCGAGTAGAATTTCCg                                                                                            >  1:264015/1‑140 (MQ=255)                                            gCGAACCGATAAATCCCTGTTCGCCGCGCGTCGCCGGATTACCATACCAGAACAAGTTCGGCCGGACAAAGCACGATTTACACAGCGCCAGGCCGGTGTACTTACGATAAACCTGAATATCAACGAGTATAATTTCCGTa                                                                                          >  2:695887/1‑140 (MQ=255)                                            gCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTa                                                                                          >  2:853753/1‑140 (MQ=255)                                            gCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTa                                                                                          >  2:241220/1‑140 (MQ=255)                                                aCCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      <  2:1170816/140‑1 (MQ=255)                                                   gATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGGGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCAc                                                                                   >  1:684056/1‑140 (MQ=255)                                                     tAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCa                                                                                 >  2:504410/1‑140 (MQ=255)                                                            cTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACt                                                                          <  1:1150382/140‑1 (MQ=255)                                                              gCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTg                                                                        >  1:1138506/1‑140 (MQ=255)                                                               cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      >  1:211123/1‑125 (MQ=255)                                                               cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      <  2:211123/125‑1 (MQ=255)                                                               cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGc                                                                       >  1:533686/1‑140 (MQ=255)                                                                 cACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGccc                                                                     <  1:1073927/140‑1 (MQ=255)                                                                   ccGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGGATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCgg                                                                   <  2:1131468/140‑1 (MQ=255)                                                                    cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCaa                                                                                   <  2:72255/123‑2 (MQ=255)                                                                    cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCAc                                                                                   >  1:72255/1‑123 (MQ=255)                                                                    cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGcc                                                                      <  2:684051/136‑1 (MQ=255)                                                                    cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGcc                                                                      >  1:684051/1‑136 (MQ=255)                                                                     gcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 >  2:758120/1‑140 (MQ=255)                                                                         gTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAg                                                             >  2:171072/1‑140 (MQ=255)                                                                                 aaTGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 <  1:994729/128‑1 (MQ=255)                                                                                 aaTGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 >  2:994729/1‑128 (MQ=255)                                                                                        aaCCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATAATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATctg                                              <  1:1205877/140‑1 (MQ=255)                                                                                            aGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCtgctgc                                          <  1:171072/140‑1 (MQ=255)                                                                                                cGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAgg                                      <  1:241220/140‑1 (MQ=255)                                                                                                    ttCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGt                                  >  1:926362/1‑140 (MQ=255)                                                                                                    ttCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGt                                  <  2:1138506/140‑1 (MQ=255)                                                                                                     tCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTa                                 <  2:328134/140‑1 (MQ=255)                                                                                                           aGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATGTCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGtt                           <  2:264015/140‑1 (MQ=255)                                                                                                                     cGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGtt                 <  2:645391/140‑1 (MQ=255)                                                                                                                       aCTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCa               >  1:1064295/1‑140 (MQ=255)                                                                                                                        cTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCAt              >  1:1028207/1‑140 (MQ=255)                                                                                                                           aCGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGa           >  2:217037/1‑140 (MQ=255)                                                                                                                               aGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATc       >  1:471812/1‑140 (MQ=255)                                                                                                                                 cgcCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCg     >  1:1101994/1‑140 (MQ=255)                                                                                                                                    cAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTtg  <  1:758120/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTG  >  NC_000913/2554803‑2555073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑