Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,572,997 A→G V170A (GTG→GCG)  eutD ← phosphate acetyltransferase EutD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,572,9970AG94.4% 171.0 / ‑0.5 54V170A (GTG→GCG) eutDphosphate acetyltransferase EutD
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (28/23);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGGGGTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCGT  >  NC_000913/2572863‑2573127
                                                                                                                                      |                                                                                                                                  
cacaGGGGTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACg                                                                                                                               >  2:1172744/1‑140 (MQ=255)
     gggTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCt                                                                                                                             >  2:62072/1‑137 (MQ=255)
     gggTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCt                                                                                                                             <  1:62072/137‑1 (MQ=255)
     gggTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCa                                                                                                                          >  2:745211/1‑140 (MQ=255)
     gggTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCa                                                                                                                          >  1:96701/1‑140 (MQ=255)
      ggTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCaa                                                                                                                         >  2:738610/1‑140 (MQ=255)
       gTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAAt                                                                                                                        >  2:736282/1‑140 (MQ=255)
          aCGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCgg                                                                                                                     >  2:797844/1‑140 (MQ=255)
          aCGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCgg                                                                                                                     >  2:643114/1‑140 (MQ=255)
               cgcTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACg                                                                                                                               >  2:58813/1‑125 (MQ=255)
               cgcTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACg                                                                                                                               <  1:58813/125‑1 (MQ=255)
               cgcTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAg                                                                                                                >  2:668650/1‑140 (MQ=255)
                 cTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGcc                                                                                                              >  1:1205172/1‑140 (MQ=255)
                        tGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGcc                                                                                                       >  2:83448/1‑140 (MQ=255)
                        tGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAGCCCAACGcc                                                                                                       >  2:313102/1‑140 (MQ=255)
                         gCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCg                                                                                                      <  1:797844/140‑1 (MQ=255)
                           tGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCggg                                                                                                    <  2:135526/140‑1 (MQ=255)
                           tGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCggg                                                                                                    <  2:178895/140‑1 (MQ=255)
                              aaaaCGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCg                                                                                                 <  2:241461/140‑1 (MQ=255)
                              aaaaCGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCg                                                                                                 <  2:596968/140‑1 (MQ=255)
                                aaCGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCt                                                                                               <  1:313102/140‑1 (MQ=255)
                                  cGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGt                                                                                             <  1:83448/140‑1 (MQ=255)
                                        cATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACtgtg                                                                                       >  1:612468/1‑140 (MQ=255)
                                             ccACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGc                                                                                     >  1:568275/1‑137 (MQ=255)
                                             ccACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGc                                                                                     <  2:568275/137‑1 (MQ=255)
                                             ccACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAAc                                                                                  >  2:195122/1‑140 (MQ=255)
                                                           tctcCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGa                                                                    <  2:96701/140‑1 (MQ=255)
                                                                ggTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAagaga                                                               <  1:1172744/140‑1 (MQ=255)
                                                                 gTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAagagag                                                              <  2:277546/140‑1 (MQ=255)
                                                                 gTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAagagag                                                              >  2:162505/1‑140 (MQ=255)
                                                                    aTGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGt                                                           >  1:1138625/1‑140 (MQ=255)
                                                                     tGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGtt                                                          >  1:291559/1‑140 (MQ=255)
                                                                      ggCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGttt                                                         >  2:232020/1‑140 (MQ=255)
                                                                      ggCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGttt                                                         >  1:1132404/1‑140 (MQ=255)
                                                                        cgcgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTa                                                       <  2:1138625/140‑1 (MQ=255)
                                                                          cgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACa                                                     >  2:244246/1‑140 (MQ=255)
                                                                                   tCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTg                                            <  1:162505/140‑1 (MQ=255)
                                                                                     ggCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCa                                          <  1:643114/140‑1 (MQ=255)
                                                                                      gCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATAGAAGAGAGCGTTTTACAGCCTGGCTGCaa                                         >  2:188319/1‑140 (MQ=255)
                                                                                          tGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCg                                     >  2:5727/1‑140 (MQ=255)
                                                                                           ggCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGa                                    <  1:745211/140‑1 (MQ=255)
                                                                                                 cgcgATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGc                              >  2:444901/1‑140 (MQ=255)
                                                                                                        tCCGCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATcc                       >  1:139617/1‑140 (MQ=255)
                                                                                                           gCCAGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGc                    >  1:471884/1‑140 (MQ=255)
                                                                                                              aGCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACg                 <  1:5727/140‑1 (MQ=255)
                                                                                                               gCTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACgc                <  2:1132404/140‑1 (MQ=255)
                                                                                                                cTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATc                        >  1:927975/1‑131 (MQ=255)
                                                                                                                cTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATc                        <  2:927975/131‑1 (MQ=255)
                                                                                                                cTGCGCCGCCGTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACgca               >  1:224099/1‑140 (MQ=255)
                                                                                                                         cgTCGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTg      <  2:818121/140‑1 (MQ=255)
                                                                                                                            cGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCg   <  2:471884/140‑1 (MQ=255)
                                                                                                                            cGGCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCg   <  1:195122/140‑1 (MQ=255)
                                                                                                                             ggCTGTGGCGCCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCGt  <  2:139617/140‑1 (MQ=255)
                                                                                                                             ggCTGTGGCACCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCGt  <  1:180403/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CACAGGGGTGACGGGCGCTACCATTGCTGGAAAACGACAGCATCGCCACGCGCGGCTCTTCTCCGGTGATGGCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGCAAGCCGATAATGCGTAATCCGGCACGCAGCACATTTGCCGT  >  NC_000913/2572863‑2573127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑