Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,643	T→C	V266A (GTT→GCT)	hyfC →	hydrogenase 4 component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,643	0	T	C	100.0%	143.5 / NA	41	V266A (GTT→GCT)	hyfC	hydrogenase 4 component C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/23);  total (18/23)

GTGCCGGGCTGGCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCT  >  NC_000913/2604508‑2604774                                                                                                                                        |                                                                                                                                    gTGCCGGGCTGGCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACg                                                                                                                                 <  1:614304/140‑1 (MQ=255) gTGCCGGGCTGGCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACg                                                                                                                                 >  1:440763/1‑140 (MQ=255)           ggCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAgg                                                                                                                       <  2:922106/140‑1 (MQ=255)                  gCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCt                                                                                                                >  1:259133/1‑140 (MQ=255)                            ggctggggctgAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTa                                                                                                      >  2:912412/1‑140 (MQ=255)                               tggggctgAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTg                                                                                                   >  1:333525/1‑140 (MQ=255)                                      gAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCaa                                                                                            <  1:912412/140‑1 (MQ=255)                                                gTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGaaaa                                                                                  <  2:233495/140‑1 (MQ=255)                                                gTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGaaaa                                                                                  <  2:715688/140‑1 (MQ=255)                                                     ggCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGc                                                                             <  1:1034656/140‑1 (MQ=255)                                                       cATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTg                                                                           >  2:457346/1‑140 (MQ=255)                                                           aCTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCAc                                                                       >  2:1029604/1‑140 (MQ=255)                                                            cTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCAcg                                                                      <  1:319123/140‑1 (MQ=255)                                                               tttGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCgg                                                                   <  1:314710/140‑1 (MQ=255)                                                                ttGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCggg                                                                  >  1:835264/1‑140 (MQ=255)                                                                 tgtgGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGc                                                                 <  1:980945/140‑1 (MQ=255)                                                                       ccTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGttttt                                                           <  1:202602/140‑1 (MQ=255)                                                                       ccTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGttttt                                                           >  2:189987/1‑140 (MQ=255)                                                                         tGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTa                                                         >  1:361201/1‑140 (MQ=255)                                                                         tGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTa                                                         <  2:426960/140‑1 (MQ=255)                                                                               tGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCAtt                                                   >  2:330730/1‑140 (MQ=255)                                                                                 cccTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAGTCGCAGAAAACACGCTGGCACGCGGGCGttttt                                                           >  2:1129068/1‑130 (MQ=255)                                                                                 cccTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGttttt                                                           <  1:1129068/130‑1 (MQ=255)                                                                                     ttGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt                                                       >  2:1111350/1‑130 (MQ=255)                                                                                     ttGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACt                                                       <  1:1111350/130‑1 (MQ=255)                                                                                     ttGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCAt                                             <  2:262446/140‑1 (MQ=255)                                                                                     ttGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCAt                                             >  2:799782/1‑140 (MQ=255)                                                                                      tGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCAtg                                            >  2:708036/1‑140 (MQ=255)                                                                                          cgcgcgcAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc                                        <  2:1217454/140‑1 (MQ=255)                                                                                          cgcgcgcAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGAc                                        <  1:1071772/140‑1 (MQ=255)                                                                                            cgcgcAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCt                                      >  2:928209/1‑140 (MQ=255)                                                                                            cgcgcAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCt                                      >  2:14119/1‑140 (MQ=255)                                                                                               gcAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCtggc                                   <  1:928209/140‑1 (MQ=255)                                                                                                              cGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtg                    <  2:67499/140‑1 (MQ=255)                                                                                                              cGCCTGCCTGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtg                    <  1:508447/140‑1 (MQ=255)                                                                                                               gcctgcctGCTGACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTtgc                   >  1:577962/1‑140 (MQ=255)                                                                                                                       gctgACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTGCTCATTCTCCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTg           <  2:370575/140‑1 (MQ=255)                                                                                                                          gACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCAt        <  2:577962/140‑1 (MQ=255)                                                                                                                          gACTTCACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCAt        <  2:440763/140‑1 (MQ=255)                                                                                                                               cACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTTACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGtc   >  2:779313/1‑140 (MQ=255)                                                                                                                               cACTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGtc   <  1:708036/140‑1 (MQ=255)                                                                                                                                aCTTGTCGCTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGtct  <  1:1029604/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GTGCCGGGCTGGCGCTAGCGAAATGGGGGCTGGGGCTGAAACAGGTCGTGATGGCATCACTGTTTGTGGCCCTGTTTCTGCCCTTTGGGCGCGCGCAAGAACTTTCTCTCGCCTGCCTGCTGACTTCACTTGTCGTTACGCTGCTCAAGGTTTTGCTGATTTTTGTACTGGCCTCAATCGCAGAAAACACGCTGGCACGCGGGCGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCT  >  NC_000913/2604508‑2604774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑