Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,744	A→G	V283V (GTA→GTG)	srmB →	ATP‑dependent RNA helicase SrmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,744	0	A	G	100.0%	183.4 / NA	52	V283V (GTA→GTG)	srmB	ATP‑dependent RNA helicase SrmB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/24);  total (28/24)

CATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGT  >  NC_000913/2713616‑2713879                                                                                                                                 |                                                                                                                                        cATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCg                                                                                                                              >  1:748144/1‑140 (MQ=255)     tGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAAc                                                                                                                          >  1:1094326/1‑140 (MQ=255)     tGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAAc                                                                                                                          >  2:741629/1‑140 (MQ=255)     tGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAAc                                                                                                                          >  1:382508/1‑140 (MQ=255)      gTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACg                                                                                                                         >  1:750701/1‑140 (MQ=255)        tAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGaa                                                                                                                       <  1:258292/140‑1 (MQ=255)         aaaaCAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAg                                                                                                                      <  2:428095/140‑1 (MQ=255)              aGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGc                                                                                                                                   >  1:1211636/1‑122 (MQ=255)              aGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGc                                                                                                                                   <  2:1211636/122‑1 (MQ=255)              aGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATc                                                                                                                 <  1:591878/140‑1 (MQ=255)               gCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCa                                                                                                                >  1:1048128/1‑140 (MQ=255)               gCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCa                                                                                                                >  2:122697/1‑140 (MQ=255)                ccGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCaa                                                                                                               >  1:701903/1‑140 (MQ=255)                 cGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAg                                                                                                              >  2:921783/1‑140 (MQ=255)                     aGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGtt                                                                                                          <  1:330742/140‑1 (MQ=255)                     aGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGtt                                                                                                          <  2:1182054/140‑1 (MQ=255)                     aGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGtt                                                                                                          <  1:921783/140‑1 (MQ=255)                               tCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAgg                                                                                                <  2:748144/140‑1 (MQ=255)                                  aTTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTcg                                                                                             <  2:489549/140‑1 (MQ=255)                                     gtgtTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGt                                                                                          <  1:731860/140‑1 (MQ=255)                                            tgCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTa                                                                                   <  1:731146/140‑1 (MQ=255)                                               gTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTg                                                                                <  2:750701/140‑1 (MQ=255)                                                     gtgagcgtgTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTa                                                                                   <  1:564392/131‑1 (MQ=255)                                                     gtgagcgtgTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTa                                                                                   >  2:564392/1‑131 (MQ=255)                                                        agcgtgTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAAcc                                                                       >  2:706136/1‑140 (MQ=255)                                                                      cTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAGGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCcgcgcg                                                         >  2:249677/1‑140 (MQ=255)                                                                      cTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCcgcgcg                                                         <  2:1094326/140‑1 (MQ=255)                                                                      cTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCcgcgcg                                                         >  1:648989/1‑140 (MQ=255)                                                                        ggCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCcgcgcgcg                                                       >  1:902570/1‑140 (MQ=255)                                                                         gCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGATGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTAGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCgg                                                      >  2:521037/1‑140 (MQ=255)                                                                          cAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGt                                                     >  2:561427/1‑140 (MQ=255)                                                                                ggCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGAc                                               >  1:737257/1‑140 (MQ=255)                                                                                 gCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACa                                              <  2:60021/140‑1 (MQ=255)                                                                                     cgcgAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTcc                                          <  1:706136/140‑1 (MQ=255)                                                                                      gcgAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCt                                         <  2:737257/140‑1 (MQ=255)                                                                                         aaGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGAc                                      >  1:1132881/1‑140 (MQ=255)                                                                                               gCATCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGc                                >  1:165905/1‑140 (MQ=255)                                                                                                  tCAACAACTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCAc                             <  2:1149360/140‑1 (MQ=255)                                                                                                      caacTGCTATCTCGAAGGTGAGATGGTGCAGGGCAGGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCt                         <  1:249677/140‑1 (MQ=255)                                                                                                      caacTGCTATCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCt                         <  1:911333/140‑1 (MQ=255)                                                                                                            cTATCTCGAAGGTGAGATGATGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTAGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACt                   <  1:521037/140‑1 (MQ=255)                                                                                                              aTCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGcc                               <  2:48673/126‑1 (MQ=255)                                                                                                              aTCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGcc                               >  1:48673/1‑126 (MQ=255)                                                                                                              aTCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCtt                        >  1:612003/1‑133 (MQ=255)                                                                                                              aTCTCGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCtt                        <  2:612003/133‑1 (MQ=255)                                                                                                                 tcGAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGat              >  2:332131/1‑140 (MQ=255)                                                                                                                   gAAGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGatat            >  1:398400/1‑140 (MQ=255)                                                                                                                    aaGGTGAGATGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATg           >  2:867110/1‑140 (MQ=255)                                                                                                                         gagaTGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCcgcg      >  2:209486/1‑140 (MQ=255)                                                                                                                          agaTGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCgcgc     >  1:468265/1‑140 (MQ=255)                                                                                                                           gaTGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCa    >  2:851382/1‑140 (MQ=255)                                                                                                                             tGGTGCAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGt  <  2:165905/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        CATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGT  >  NC_000913/2713616‑2713879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑