Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,998	T→C	F68F (TTT→TTC)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,998	0	T	C	98.5%	235.3 / ‑4.4	68	F68F (TTT→TTC)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (35/32);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCT  >  NC_000913/2751865‑2752133                                                                                                                                      |                                                                                                                                        tGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTg                                                                                                                                   <  1:177568/140‑1 (MQ=255)       taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGAt                                                                                                                             >  2:226216/1‑140 (MQ=255)        aataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATa                                                                                                                            <  2:119800/140‑1 (MQ=255)             cTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCa                                                                                                                       >  1:1086206/1‑140 (MQ=255)              tGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGGCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAg                                                                                                                      >  2:844735/1‑140 (MQ=255)              tGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAg                                                                                                                      <  2:317350/140‑1 (MQ=255)               ggCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGc                                                                                                                     >  1:606942/1‑140 (MQ=255)               ggCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGc                                                                                                                     >  2:519075/1‑140 (MQ=255)                gCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGa                                                                                                                              >  2:538300/1‑130 (MQ=255)                gCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGa                                                                                                                              <  1:538300/130‑1 (MQ=255)                  gagaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGc                                                                                                                  >  2:158202/1‑140 (MQ=255)                   agaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGct                                                                                                                 <  2:365933/140‑1 (MQ=255)                          cgcgGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCgct                                                                                                          >  1:861796/1‑140 (MQ=255)                                    tCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGgaaga                                                                                                >  1:720498/1‑140 (MQ=255)                                        ttGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAc                                                                                            <  2:720498/140‑1 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  1:1117704/1‑140 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  2:1053395/1‑140 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  2:231955/1‑140 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  2:302885/1‑140 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  1:848102/1‑140 (MQ=255)                                         tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAcc                                                                                           >  1:603968/1‑140 (MQ=255)                                          gCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCa                                                                                          >  1:243821/1‑140 (MQ=255)                                            aaTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGc                                                                                        >  2:719250/1‑140 (MQ=255)                                              tAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCtt                                                                                      >  1:476711/1‑140 (MQ=255)                                              tAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCtt                                                                                      >  1:949492/1‑140 (MQ=255)                                               aGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTg                                                                                     >  1:1082821/1‑140 (MQ=255)                                               aGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTg                                                                                     <  2:848102/140‑1 (MQ=255)                                                    cccTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                                                                <  1:74567/140‑1 (MQ=255)                                                      cTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACgg                                                                              >  2:131550/1‑140 (MQ=255)                                                        cGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                                                                <  1:1008996/136‑1 (MQ=255)                                                        cGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                                                                >  2:1008996/1‑136 (MQ=255)                                                        cGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGc                                                                            >  2:977124/1‑140 (MQ=255)                                                         ggTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCa                                                                           <  1:719250/140‑1 (MQ=255)                                                          gTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACg                                                                               <  2:314886/135‑1 (MQ=255)                                                          gTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACg                                                                               >  1:314886/1‑135 (MQ=255)                                                          gTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCAt                                                                          <  1:302885/140‑1 (MQ=255)                                                              ttGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAAt                                                                      <  2:1117704/140‑1 (MQ=255)                                                                  ctcGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGttt                                                                  <  2:901533/140‑1 (MQ=255)                                                                         gtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTtcgt                                                           >  1:17652/1‑140 (MQ=255)                                                                          tCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTtcgtc                                                          <  1:1053395/140‑1 (MQ=255)                                                                           cgcgCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTtcgtcg                                                         >  1:295510/1‑140 (MQ=255)                                                                            gcgcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTcgc                                                        <  1:158202/140‑1 (MQ=255)                                                                             cgcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTcgcg                                                       >  1:233414/1‑140 (MQ=255)                                                                               cTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTg                                                     <  2:603968/140‑1 (MQ=255)                                                                                    gCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATcag                                                <  2:876771/140‑1 (MQ=255)                                                                                              tGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGt                                      >  1:2138/1‑140 (MQ=255)                                                                                                     cGGCGCTGCTCGCGCTGCCCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTccc                               <  2:1082821/140‑1 (MQ=255)                                                                                                     cGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTccc                               <  2:861796/140‑1 (MQ=255)                                                                                                       gcgcTGCTCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGt                             >  1:124710/1‑140 (MQ=255)                                                                                                          gtgctCGCGCTGACCTGTGAGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGATGGTTGGAAGAAAACCAGCTTGAAGACTGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGt                          <  2:303860/139‑1 (MQ=255)                                                                                                             ctCGCGCTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTtc                       >  1:471239/1‑140 (MQ=255)                                                                                                                gcgcTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTtcatc                    <  2:17652/140‑1 (MQ=255)                                                                                                                gcgcTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTtcatc                    <  2:201479/140‑1 (MQ=255)                                                                                                                gcgcTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTtcatc                    <  1:519075/140‑1 (MQ=255)                                                                                                                gcgcTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTtcatc                    <  1:602827/140‑1 (MQ=255)                                                                                                                  gcTGACCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCaa                  >  2:1065696/1‑140 (MQ=255)                                                                                                                      aCCTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGt              <  2:606942/140‑1 (MQ=255)                                                                                                                       ccTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTa             >  1:984033/1‑140 (MQ=255)                                                                                                                       ccTGTGCGCCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTa             <  1:977124/140‑1 (MQ=255)                                                                                                                            gcgcCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCt        >  1:133354/1‑140 (MQ=255)                                                                                                                            gcgcCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGATGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCt        >  1:83765/1‑140 (MQ=255)                                                                                                                             cgcCCGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTg       <  1:2486/140‑1 (MQ=255)                                                                                                                                 cGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTcc   <  2:2138/140‑1 (MQ=255)                                                                                                                                 cGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTcc   <  2:124710/140‑1 (MQ=255)                                                                                                                                 cGTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTcc   <  1:231955/140‑1 (MQ=255)                                                                                                                                  gTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCATCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCct  <  1:971528/140‑1 (MQ=255)                                                                                                                                  gTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGt      <  2:588714/136‑1 (MQ=255)                                                                                                                                  gTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGt      >  1:588714/1‑136 (MQ=255)                                                                                                                                  gTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTcc   >  1:985742/1‑139 (MQ=255)                                                                                                                                  gTTTCTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTcc   <  2:985742/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCT  >  NC_000913/2751865‑2752133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑