Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,779,615	T→C	S1038G (AGT→GGT)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,779,615	0	T	C	100.0%	189.4 / NA	53	S1038G (AGT→GGT)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/19);  total (34/19)

TAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCGTCATTA  >  NC_000913/2779479‑2779746                                                                                                                                         |                                                                                                                                    tAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGcc                                                                                                                                  <  2:1210505/140‑1 (MQ=255)      aCCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGc                                                                                                                             >  1:882432/1‑140 (MQ=255)      aCCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGc                                                                                                                             >  1:16492/1‑140 (MQ=255)      aCCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGc                                                                                                                             >  1:1215220/1‑140 (MQ=255)        cGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTg                                                                                                                           >  1:229335/1‑140 (MQ=255)        cGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTg                                                                                                                           >  1:1137013/1‑140 (MQ=255)           tAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCa                                                                                                                        >  1:436032/1‑140 (MQ=255)                  ggCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCa                                                                                                                 >  2:173985/1‑140 (MQ=255)                  ggCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCa                                                                                                                 >  2:1153459/1‑140 (MQ=255)                       gcgcTGCTGTTTGTGCTGAC‑AATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCgg                                                                                                           >  2:580562/1‑140 (MQ=255)                           tgctgTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCaa                                                                                                        >  2:189735/1‑140 (MQ=255)                                       tGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTGCCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGa                                                                                            <  1:927830/140‑1 (MQ=255)                                       tGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGa                                                                                            >  1:545184/1‑140 (MQ=255)                                       tGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGa                                                                                            <  2:490493/140‑1 (MQ=255)                                         aCAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACg                                                                                          <  1:173985/140‑1 (MQ=255)                                         aCAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACg                                                                                          <  1:1153459/140‑1 (MQ=255)                                             aTAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCAt                                                                                      <  2:229335/140‑1 (MQ=255)                                                  cATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTccc                                                                                 <  2:436032/140‑1 (MQ=255)                                                   aTCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCa                                                                                >  2:84010/1‑140 (MQ=255)                                                          tcgtTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCaa                                                                         >  2:816253/1‑140 (MQ=255)                                                          tcgtTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCaa                                                                         <  2:545184/140‑1 (MQ=255)                                                             ttGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGt                                                                      <  1:580562/140‑1 (MQ=255)                                                             ttGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGt                                                                      <  1:84010/140‑1 (MQ=255)                                                                   aGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCt                                                                <  1:1153574/140‑1 (MQ=255)                                                                     cAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTAt                                                              >  1:1115368/1‑140 (MQ=255)                                                                                  ctATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGAc                                                 >  1:1168639/1‑140 (MQ=255)                                                                                    atatTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGATACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCg                                               >  2:692482/1‑140 (MQ=255)                                                                                      atTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGcc                                             >  1:1190266/1‑140 (MQ=255)                                                                                         cGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCt                                          <  2:1115368/140‑1 (MQ=255)                                                                                         cGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCt                                          >  2:168153/1‑140 (MQ=255)                                                                                         cGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCt                                          >  2:97939/1‑140 (MQ=255)                                                                                          gCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCtt                                         >  2:871344/1‑140 (MQ=255)                                                                                           cAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTc                                        >  2:1204470/1‑140 (MQ=255)                                                                                             gTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGa                                      >  2:338531/1‑140 (MQ=255)                                                                                              ttGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAg                                     <  1:1204470/140‑1 (MQ=255)                                                                                                    gTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAAc                               >  2:538686/1‑140 (MQ=255)                                                                                                      aCCTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTg                             >  2:445948/1‑140 (MQ=255)                                                                                                        cTGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGc                           <  1:97939/140‑1 (MQ=255)                                                                                                         tGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCg                          <  2:1016504/140‑1 (MQ=255)                                                                                                         tGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCg                          <  1:1091266/140‑1 (MQ=255)                                                                                                         tGCACAGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCg                          <  1:445948/140‑1 (MQ=255)                                                                                                           cacaGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTa                        >  2:1146880/1‑140 (MQ=255)                                                                                                              aGGAATTATTGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACa                     >  1:448704/1‑140 (MQ=255)                                                                                                               ggtattattGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTTGCGCTATCGGTAATCAGGACCGCCCCTTCGAGTTTAACTGGCGTAACAt                    >  2:969128/4‑140 (MQ=255)                                                                                                                     attGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATccc              >  1:1032467/1‑140 (MQ=255)                                                                                                                     attGCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATccc              >  2:587819/1‑140 (MQ=255)                                                                                                                        gCTGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTg           >  2:947325/1‑140 (MQ=255)                                                                                                                          tGTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGc         >  1:849665/1‑140 (MQ=255)                                                                                                                           gTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCg                          >  2:1056101/1‑122 (MQ=255)                                                                                                                           gTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCg                          <  1:1056101/122‑1 (MQ=255)                                                                                                                           gTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCg        <  2:16492/140‑1 (MQ=255)                                                                                                                           gTTAAGCCAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCg        >  2:53707/1‑140 (MQ=255)                                                                                                                                 ccAGACACCGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCGTCATTa  >  2:667110/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TAGATACCGTTAGTTGTGGCAGGCGCTGCTGTTTGTGCTGACAAATAAACATCACCGTCGTTGAGTAGCAAACTGTTTACTCTATATTCGCAGTTGCTGGTACCTGCACAGGAATTATTGCTGTTAAGCCAGACACTGCCCCGGCTGGCAGCCGTCAGGTCGGCAAGCGTGGTATCGACGCCATTTCCCAGAGTCAATGTCGCGCTATCGGTAATCCGGACCGCCCCTTCGAGTTTAACTGGCGTAACATTATCCCGTGGCGTCATTA  >  NC_000913/2779479‑2779746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑