Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,583	T→C	G82G (GGT→GGC)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,583	0	T	C	100.0%	196.0 / NA	55	G82G (GGT→GGC)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG  >  NC_000913/2854459‑2854718                                                                                                                             |                                                                                                                                        cTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCAtt                                                                                                                          >  2:350515/1‑140 (MQ=255)      cTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGt                                                                                                                      >  2:267142/1‑139 (MQ=255)      cTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGt                                                                                                                      <  1:267142/139‑1 (MQ=255)         gCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTcgcg                                                                                                                  <  2:474242/140‑1 (MQ=255)           cGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGcc                                                                                                                <  2:501323/140‑1 (MQ=255)              cAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTg                                                                                                             >  2:444159/1‑140 (MQ=255)                 cgcgGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCCCACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATa                                                                                                          <  2:310836/140‑1 (MQ=255)                   cgGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACg                                                                                                        <  2:425368/140‑1 (MQ=255)                        aTTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCa                                                                                                   <  1:350515/140‑1 (MQ=255)                                gtgGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGtt                                                                                           >  2:1141282/1‑140 (MQ=255)                                   gCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACg                                                                                        >  2:513072/1‑140 (MQ=255)                                   gCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACg                                                                                        >  2:63537/1‑140 (MQ=255)                                    cAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACga                                                                                       <  1:959530/140‑1 (MQ=255)                                      aGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACgaag                                                                                     >  1:313116/1‑140 (MQ=255)                                            gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAAtttt                                                                               >  2:392825/1‑140 (MQ=255)                                                  aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTtgtg                                                                            <  1:93228/137‑1 (MQ=255)                                                  aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTtgtg                                                                            >  2:93228/1‑137 (MQ=255)                                                  aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGaaa                                                                         >  1:400490/1‑140 (MQ=255)                                                            tattaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCa                                                               >  2:1203585/1‑140 (MQ=255)                                                             attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAg                                                              <  1:1141282/140‑1 (MQ=255)                                                                  gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGa                                                         <  1:63537/140‑1 (MQ=255)                                                                    gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  1:871489/1‑140 (MQ=255)                                                                    gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  2:477133/1‑140 (MQ=255)                                                                    gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  1:14572/1‑140 (MQ=255)                                                                      cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  1:823369/1‑140 (MQ=255)                                                                      cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  2:289984/1‑140 (MQ=255)                                                                      cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  1:952992/1‑140 (MQ=255)                                                                       gcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAg                                                    <  2:14572/140‑1 (MQ=255)                                                                         gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGt                                                  <  2:952992/140‑1 (MQ=255)                                                                              aaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCt                                             <  2:355088/140‑1 (MQ=255)                                                                               aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCta                                            <  1:423076/140‑1 (MQ=255)                                                                                aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGc                                                      <  2:483418/129‑1 (MQ=255)                                                                                aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGc                                                      >  1:483418/1‑129 (MQ=255)                                                                                aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCtat                                           >  1:847298/1‑140 (MQ=255)                                                                                  acacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         >  2:398203/1‑140 (MQ=255)                                                                                   cacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAcc                                        <  1:513072/140‑1 (MQ=255)                                                                                      ccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaa                                     >  2:634042/1‑140 (MQ=255)                                                                                         cATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                  <  2:269917/140‑1 (MQ=255)                                                                                         cATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                  <  1:444159/140‑1 (MQ=255)                                                                                             aaaTATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              <  2:1058406/140‑1 (MQ=255)                                                                                              aaTATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                             <  1:392825/140‑1 (MQ=255)                                                                                               atatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTc                            >  2:571352/1‑140 (MQ=255)                                                                                                tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         <  2:684512/126‑1 (MQ=255)                                                                                                tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         >  1:684512/1‑126 (MQ=255)                                                                                                tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                           <  1:571352/140‑1 (MQ=255)                                                                                                    aaGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                       <  1:1203585/140‑1 (MQ=255)                                                                                                    aaGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                       >  1:734833/1‑140 (MQ=255)                                                                                                       aCGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              >  1:465824/1‑130 (MQ=255)                                                                                                       aCGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              <  2:465824/130‑1 (MQ=255)                                                                                                                ttCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCa           >  1:118513/1‑140 (MQ=255)                                                                                                                    ggCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg       <  2:734833/140‑1 (MQ=255)                                                                                                                     gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCt                  >  2:485506/1‑128 (MQ=255)                                                                                                                     gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCt                  <  1:485506/128‑1 (MQ=255)                                                                                                                     gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggc      >  2:1139569/1‑140 (MQ=255)                                                                                                                         acGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGgcggcg  <  2:823369/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                        CTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG  >  NC_000913/2854459‑2854718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑