Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,870,459	T→C	E282E (GAA→GAG)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,870,459	0	T	C	100.0%	283.3 / NA	79	E282E (GAA→GAG)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/39);  total (40/39)

CCCGCCGGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTC  >  NC_000913/2870325‑2870590                                                                                                                                       |                                                                                                                                    cccGCCGGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTgc                                                                                                                                >  2:756364/1‑140 (MQ=255) cccGCCGGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTgc                                                                                                                                >  2:1049482/1‑140 (MQ=255)    gccgGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTgcggc                                                                                                                             <  2:679235/140‑1 (MQ=255)      cgGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGa                                                                                                                           >  2:806305/1‑140 (MQ=255)       ggAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGAc                                                                                                                          <  2:852735/140‑1 (MQ=255)           gCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAgct                                                                                                                      >  2:201533/1‑140 (MQ=255)              aGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAgctgct                                                                                                                   <  1:475801/140‑1 (MQ=255)                 aaCGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGt                                                                                                                <  2:1095644/140‑1 (MQ=255)                    ggATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCg                                                                                                             <  1:1049482/140‑1 (MQ=255)                     gATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGa                                                                                                            <  2:965765/140‑1 (MQ=255)                             cGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAgc                                                                                                    >  1:355077/1‑140 (MQ=255)                              ggTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAgcg                                                                                                   >  2:1124156/1‑140 (MQ=255)                                tGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCt                                                                                                 >  1:1025537/1‑140 (MQ=255)                                tGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCt                                                                                                 >  2:1008800/1‑140 (MQ=255)                                tGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCt                                                                                                 >  1:284765/1‑140 (MQ=255)                                  acatCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTc                                                                                               >  1:490591/4‑140 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGa                                                                                                            >  2:655305/1‑127 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGa                                                                                                            <  1:655305/127‑1 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAgcgc                                                                                                  <  1:1183104/137‑1 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAgcgc                                                                                                  >  2:1183104/1‑137 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTc                                                                                               >  2:688889/1‑140 (MQ=255)                                  aCGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTc                                                                                               >  2:335789/1‑140 (MQ=255)                                   cGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGTGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCa                                                                                              >  2:190982/1‑140 (MQ=255)                                       aTCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCt                                                                                          <  1:587500/140‑1 (MQ=255)                                            accaATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGtt                                                                                     >  2:1102035/1‑140 (MQ=255)                                            accaATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGtt                                                                                     <  1:1124156/140‑1 (MQ=255)                                              caATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTcc                                                                                   <  1:756364/140‑1 (MQ=255)                                                 ttCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCa                                                                                <  2:261338/140‑1 (MQ=255)                                                  tCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGt                                                                                      >  1:906550/1‑133 (MQ=255)                                                  tCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGt                                                                                      <  2:906550/133‑1 (MQ=255)                                                   ccAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGtt                                                                                     >  1:1048011/1‑133 (MQ=255)                                                   ccAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGtt                                                                                     <  2:1048011/133‑1 (MQ=255)                                                      gCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGc                                                                           <  1:770087/140‑1 (MQ=255)                                                        ttAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         <  2:278872/140‑1 (MQ=255)                                                        ttAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         <  1:1001846/140‑1 (MQ=255)                                                          aaTTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACt                                                                       >  2:1052602/1‑140 (MQ=255)                                                           aTTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTg                                                                      >  1:1038191/1‑140 (MQ=255)                                                                tgCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTgg                                                                 <  1:989607/140‑1 (MQ=255)                                                                tgCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTgg                                                                 <  1:334008/140‑1 (MQ=255)                                                                    gATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAAt                                                             >  2:289320/1‑140 (MQ=255)                                                                    gATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAAt                                                             >  1:688966/1‑140 (MQ=255)                                                                        cagcagCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATgcgg                                                         <  1:201533/140‑1 (MQ=255)                                                                         agcagcATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         <  1:460901/123‑1 (MQ=255)                                                                         agcagcATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         <  1:339097/123‑1 (MQ=255)                                                                         agcagcATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         >  2:339097/1‑123 (MQ=255)                                                                         agcagcATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCa                                                                         >  2:460901/1‑123 (MQ=255)                                                                             gcaTCGCTCTGCGCGCGGCTTCAACTTTTTCGCACACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGtt                                                    <  2:569827/140‑1 (MQ=255)                                                                              caTCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGttt                                                   >  1:790896/1‑139 (MQ=255)                                                                              caTCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTa                                                   >  2:1081296/1‑140 (MQ=255)                                                                                tCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatc                                                 >  1:1064495/1‑140 (MQ=255)                                                                                 cGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatca                                                >  1:697372/1‑140 (MQ=255)                                                                                  gCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatcat                                               <  1:806305/140‑1 (MQ=255)                                                                                   ctctGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatcatc                                              <  1:527703/140‑1 (MQ=255)                                                                                   ctctGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatcatc                                              <  2:272902/140‑1 (MQ=255)                                                                                   ctctGCGCGCGGCTTCAACTTTTTCGCACACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTatcatc                                              >  1:446937/1‑140 (MQ=255)                                                                                    tctGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTAtcatca                                             >  2:107594/1‑140 (MQ=255)                                                                                     ctGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCaa                                            <  1:996972/140‑1 (MQ=255)                                                                                        cgcgcgGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTTCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAActc                                         >  1:146900/1‑140 (MQ=255)                                                                                        cgcgcgGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAActc                                         >  2:772088/1‑140 (MQ=255)                                                                                          cgcgGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCtt                                       <  2:741626/140‑1 (MQ=255)                                                                                          cgcgGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTACTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACCCtt                                       >  1:496818/1‑140 (MQ=255)                                                                                           gcgGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCttt                                      >  1:277575/1‑140 (MQ=255)                                                                                                  cAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTg                               >  2:222927/1‑140 (MQ=255)                                                                                                     cTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacg                            <  1:107594/140‑1 (MQ=255)                                                                                                     cTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacg                            <  2:1025537/140‑1 (MQ=255)                                                                                                     cTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacg                            <  2:697372/140‑1 (MQ=255)                                                                                                      tttttCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGTGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgc                           <  1:190982/140‑1 (MQ=255)                                                                                                      tttttCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgc                           <  1:222927/140‑1 (MQ=255)                                                                                                        tttCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcga                         <  2:349145/140‑1 (MQ=255)                                                                                                        tttCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcga                         <  2:1038191/140‑1 (MQ=255)                                                                                                        tttCGCGCACCAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTgacgcga                         <  1:637780/140‑1 (MQ=255)                                                                                                                  cAGTAAAGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAAtt               >  2:1168741/1‑140 (MQ=255)                                                                                                                      aaaGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGc           <  1:1052602/140‑1 (MQ=255)                                                                                                                        aGCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCa         <  2:688966/140‑1 (MQ=255)                                                                                                                         gCTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAg        <  2:94400/140‑1 (MQ=255)                                                                                                                          cTTGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGt       >  2:324315/1‑140 (MQ=255)                                                                                                                            tGTAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttc     >  2:80264/1‑140 (MQ=255)                                                                                                                              tAATTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttctt   >  2:420647/1‑140 (MQ=255)                                                                                                                               aaTTCAGTCTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGttcttc  >  1:633158/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    CCCGCCGGAAGCCAGAAACGGATCTCTACGGTGACGTCATCCCACCAATTCCAGCTTAATTGTTGCGGATACAGCAGCATCGCTCTGCGCGCGGCTTCAACTTTTTCGCGCACCAGTAAAGCTTGTAATTCAGTTTCTGCGGCGACAGCTGCTTGTTCGAATGCCAGCGCTTCACGCTGAGTTCCCCATTCGCCACTGCCTGGCAATGCGGCGGTTATCATCAACTCTTTATCGTTGACGCGACGCTGTAATTCCGCCAGTTCTTC  >  NC_000913/2870325‑2870590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑