Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,057	A→G	E27E (GAA→GAG)	gpt →	xanthine‑guanine phsophoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,057	0	A	G	100.0%	328.6 / NA	92	E27E (GAA→GAG)	gpt	xanthine‑guanine phsophoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/46);  total (46/46)

CCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAA  >  NC_000913/255920‑256192                                                                                                                                          |                                                                                                                                        ccTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCa                                                                                                                                       >  2:213427/1‑140 (MQ=255) ccTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCa                                                                                                                                       >  2:373307/1‑140 (MQ=255)  cTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCaa                                                                                                                                      <  2:6767/140‑1 (MQ=255)   tATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAAt                                                                                                                                     <  2:850085/140‑1 (MQ=255)           ccTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGc                                                                                                                             >  2:823454/1‑140 (MQ=255)               gAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAgg                                                                                                                              >  1:821126/1‑135 (MQ=255)               gAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAgg                                                                                                                              <  2:821126/135‑1 (MQ=255)               gAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCatta                                                                                                                         >  2:123669/1‑140 (MQ=255)               gAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCatta                                                                                                                         <  2:150406/140‑1 (MQ=255)                          aaGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGc                                                                                                              <  1:944231/140‑1 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCTGTAAGCCGTgg                                                                                                         <  1:262012/140‑1 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                                         >  1:727367/1‑140 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                                         >  2:716843/1‑140 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                                         >  2:911679/1‑140 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                                         >  1:1184838/1‑140 (MQ=255)                               gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTAAGCAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                                         <  1:764994/140‑1 (MQ=255)                                tAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGc                                                                                                        >  2:548966/1‑140 (MQ=255)                                 aGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCg                                                                                                       <  1:373307/140‑1 (MQ=255)                                 aGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCg                                                                                                       >  2:881430/1‑140 (MQ=255)                                   aTAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGt                                                                                                     >  1:247111/1‑140 (MQ=255)                                   aTAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGt                                                                                                     <  2:188302/140‑1 (MQ=255)                                          ggCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGt                                                                                                     >  1:285981/1‑133 (MQ=255)                                          ggCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGt                                                                                                     <  2:285981/133‑1 (MQ=255)                                           gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             >  2:251956/1‑140 (MQ=255)                                           gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             >  2:886109/1‑140 (MQ=255)                                           gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             >  2:1003605/1‑140 (MQ=255)                                           gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             <  2:970798/140‑1 (MQ=255)                                           gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             >  1:547589/1‑140 (MQ=255)                                            cTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCg                                                                                            >  1:419155/1‑140 (MQ=255)                                             tGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCgg                                                                                           >  2:65043/1‑140 (MQ=255)                                             tGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCgg                                                                                           <  1:823454/140‑1 (MQ=255)                                             tGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCgg                                                                                           >  1:93840/1‑140 (MQ=255)                                              gggACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCggg                                                                                          <  1:734825/140‑1 (MQ=255)                                                    cTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGtt                                                                                    <  2:914515/140‑1 (MQ=255)                                                     ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             >  1:1144557/1‑130 (MQ=255)                                                     ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTAcc                                                                                             <  2:1144557/130‑1 (MQ=255)                                                     ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTa                                                                                   >  2:278903/1‑140 (MQ=255)                                                       cacaTGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACt                                                                                 <  1:716843/140‑1 (MQ=255)                                                       cacaTGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACt                                                                                 <  2:419155/140‑1 (MQ=255)                                                       cacaTGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACt                                                                                 <  2:195234/140‑1 (MQ=255)                                                           tGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGgcg                                                                             <  1:886109/140‑1 (MQ=255)                                                             aGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGcgcg                                                                           >  1:724124/1‑140 (MQ=255)                                                             aGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGcgcg                                                                           >  1:848227/1‑140 (MQ=255)                                                               cGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGgcgc                                                                            >  2:79884/1‑137 (MQ=255)                                                               cGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGgcgc                                                                            <  1:79884/137‑1 (MQ=255)                                                                 aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                                       >  1:161154/1‑140 (MQ=255)                                                                 aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                                       >  1:282004/1‑140 (MQ=255)                                                                 aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                                       >  2:813256/1‑140 (MQ=255)                                                                 aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                                       >  1:14681/1‑140 (MQ=255)                                                                 aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                                       >  1:890804/1‑140 (MQ=255)                                                                  aaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAAc                                                                      <  2:93840/140‑1 (MQ=255)                                                                   aaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACt                                                                     <  1:123669/140‑1 (MQ=255)                                                                   aaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACt                                                                     <  2:901954/140‑1 (MQ=255)                                                                   aaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACt                                                                     <  1:245704/140‑1 (MQ=255)                                                                        cATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTa                                                                <  2:231271/140‑1 (MQ=255)                                                                         aTCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTAt                                                               <  1:548966/140‑1 (MQ=255)                                                                           cGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTGCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTc                                                             <  2:1063703/140‑1 (MQ=255)                                                                            gTCACCTGGGACATGTTGCAGATCCATGCACGTAACCTCGCAACCCGACTGATCCCTTCGGAACAACGGCAAGGCATTATTGCCGTAGGCCGTGGCGGTCTGGTACCGGGTACGTTACTGGCGCGTGAACTGGGTATTcc                                                            >  2:440711/1‑139 (MQ=255)                                                                             tCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGt                                                           <  1:813256/140‑1 (MQ=255)                                                                             tCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGt                                                           <  1:213427/140‑1 (MQ=255)                                                                             tCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGt                                                           >  1:411078/1‑140 (MQ=255)                                                                             tCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGt                                                           <  2:411078/140‑1 (MQ=255)                                                                                    ggACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCAGGTACCGGGGGCGTTTCTGGCGCGTGAACTGGGGATTCGtgctggtg                                                    >  2:900550/1‑133 (MQ=255)                                                                                       cATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATa                                                 >  2:390148/1‑140 (MQ=255)                                                                                       cATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATAGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATa                                                 <  1:622010/140‑1 (MQ=255)                                                                                         tGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATAcc                                               <  1:358177/140‑1 (MQ=255)                                                                                         tGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATAGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGGGAACTGGGGATTCGTCATGTTGATAcc                                               >  2:622010/1‑140 (MQ=255)                                                                                          gTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCg                                              <  1:881430/140‑1 (MQ=255)                                                                                             gCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGttt                                           <  2:282004/140‑1 (MQ=255)                                                                                               aGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGt                                         <  2:14681/140‑1 (MQ=255)                                                                                                 aTCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTAt                                       >  2:800095/1‑140 (MQ=255)                                                                                                  tCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTAtt                                      <  2:547589/140‑1 (MQ=255)                                                                                                     aTGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTcc                                   >  1:138494/1‑140 (MQ=255)                                                                                                      tGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCa                                  >  1:152323/1‑140 (MQ=255)                                                                                                      tGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCa                                  >  2:544130/1‑140 (MQ=255)                                                                                                         aCGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCt                               <  1:390148/140‑1 (MQ=255)                                                                                                          cGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTa                              <  2:848227/140‑1 (MQ=255)                                                                                                          cGTAAACTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTa                              <  1:911679/140‑1 (MQ=255)                                                                                                             aaaCTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTTGTCATGTCGATACCGTTTGTATTTCCAGCTACgc                           >  1:417114/1‑139 (MQ=255)                                                                                                             aaaCTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGt                                         <  2:321314/126‑1 (MQ=255)                                                                                                             aaaCTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGt                                         >  1:321314/1‑126 (MQ=255)                                                                                                             aaaCTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGc                                >  2:641505/1‑135 (MQ=255)                                                                                                             aaaCTCGCAAGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGc                                <  1:641505/135‑1 (MQ=255)                                                                                                                     aaGCCGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACa                   >  2:351797/1‑140 (MQ=255)                                                                                                                        ccGACTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAAcc                <  2:951082/140‑1 (MQ=255)                                                                                                                           aCTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgc             >  2:642462/1‑140 (MQ=255)                                                                                                                            cTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg            <  1:251956/140‑1 (MQ=255)                                                                                                                            cTGATGCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg            <  1:642462/140‑1 (MQ=255)                                                                                                                                 gCCTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGc       >  1:915828/1‑140 (MQ=255)                                                                                                                                  ccTTCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCt      >  1:196456/1‑140 (MQ=255)                                                                                                                                    ttCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTa    >  1:782155/1‑140 (MQ=255)                                                                                                                                    ttCTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTa    <  2:890804/140‑1 (MQ=255)                                                                                                                                      cTGAGCAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTaaa  <  2:138494/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAA  >  NC_000913/255920‑256192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑