Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,542	T→C	D402G (GAC→GGC)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,542	0	T	C	100.0%	144.8 / NA	42	D402G (GAC→GGC)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

TCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGT  >  NC_000913/2919406‑2919676                                                                                                                                         |                                                                                                                                       tCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCccagccca                                                                                                                                     <  1:86482/140‑1 (MQ=255)   aaTGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCccagcccagt                                                                                                                                   >  2:718385/1‑140 (MQ=255)    aTGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGtt                                                                                                                                  >  1:747674/1‑140 (MQ=255)         ggCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACg                                                                                                                             >  1:953122/1‑140 (MQ=255)          gCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGc                                                                                                                            >  2:791959/1‑140 (MQ=255)                  cGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCg                                                                                                                    <  1:189214/140‑1 (MQ=255)                    ggACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCggg                                                                                                                  <  1:863453/140‑1 (MQ=255)                     gACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCggg                                                                                                                  <  2:386789/139‑1 (MQ=255)                     gACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCggg                                                                                                                  >  1:386789/1‑139 (MQ=255)                           tACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCa                                                                                                           >  2:541886/1‑140 (MQ=255)                             cGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAgg                                                                                                         <  1:133129/140‑1 (MQ=255)                                  aaaGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACgg                                                                                                    <  1:112567/140‑1 (MQ=255)                                              ggggATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt                                                                                        <  1:314113/140‑1 (MQ=255)                                              ggggATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt                                                                                        <  1:550061/140‑1 (MQ=255)                                                   tCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTa                                                                                   <  1:480903/140‑1 (MQ=255)                                                               tCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGa                                                                       >  2:659161/1‑140 (MQ=255)                                                                  gACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAttt                                                                    >  1:267532/1‑140 (MQ=255)                                                                     cTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTgg                                                                 >  1:574746/1‑140 (MQ=255)                                                                     cTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTgg                                                                 >  1:165710/1‑140 (MQ=255)                                                                       gCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGt                                                               <  2:315713/140‑1 (MQ=255)                                                                       gCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGt                                                               <  1:229632/140‑1 (MQ=255)                                                                            gtTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc                                                          <  1:659161/140‑1 (MQ=255)                                                                            gtTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGc                                                          >  1:428288/1‑140 (MQ=255)                                                                             ttACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCg                                                         >  2:1048398/1‑140 (MQ=255)                                                                             ttACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCg                                                         >  2:591310/1‑140 (MQ=255)                                                                                  cgcACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGTAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAAt                                                    >  1:968118/1‑140 (MQ=255)                                                                                  cgcACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAAt                                                    >  2:40371/1‑140 (MQ=255)                                                                                       cGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc                                               >  1:891381/1‑140 (MQ=255)                                                                                         ccAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCcctc                                             <  1:935671/140‑1 (MQ=255)                                                                                                gACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCaa                                      <  1:1048398/140‑1 (MQ=255)                                                                                                 aCGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCaaa                                     >  1:1136543/1‑140 (MQ=255)                                                                                                      tataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAg                                <  1:591310/140‑1 (MQ=255)                                                                                                       ataGGCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGt                               <  1:1034645/140‑1 (MQ=255)                                                                                                           gCCTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGt                           >  1:768074/1‑140 (MQ=255)                                                                                                             cTCATGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTAt                         >  2:761563/1‑140 (MQ=255)                                                                                                                aTGTGCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGa                      <  1:791959/140‑1 (MQ=255)                                                                                                                 tgtgCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAt                     <  1:784655/140‑1 (MQ=255)                                                                                                                 tgtgCCTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGAt                     <  1:449575/140‑1 (MQ=255)                                                                                                                      cTTTTGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCgg                <  1:541886/140‑1 (MQ=255)                                                                                                                          tGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCgg                >  1:925395/1‑136 (MQ=255)                                                                                                                          tGTACCTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCgg                <  2:925395/136‑1 (MQ=255)                                                                                                                               cTGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTAc       >  1:269538/1‑140 (MQ=255)                                                                                                                                tgatCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTAcc      >  2:477630/4‑140 (MQ=255)                                                                                                                                tGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTAcc      >  2:200663/1‑140 (MQ=255)                                                                                                                                tGTTCCCAGCCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTAcc      <  2:165710/140‑1 (MQ=255)                                                                                                                                    cccagcccagTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGt  >  1:421482/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCTCCTGCCAAATCCAGTGGGTATCGATAGCGGTAGGATTACCCGGT  >  NC_000913/2919406‑2919676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑