Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,014,810	G→A	P83L (CCC→CTC)	yqeC ←	uncharacterized protein YqeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,014,810	0	G	A	100.0%	231.6 / NA	66	P83L (CCC→CTC)	yqeC	uncharacterized protein YqeC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/31);  total (35/31)

TCCACGCGAGCCATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGGGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAG  >  NC_000913/3014677‑3014937                                                                                                                                       |                                                                                                                                tCCACGCGAGCCATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATg                                                                                                                           >  2:98846/1‑140 (MQ=255)       cgAGCCATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAagag                                                                                                                     <  2:298842/140‑1 (MQ=255)          gCCATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACg                                                                                                                  <  2:351359/140‑1 (MQ=255)           ccATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGc                                                                                                                 <  1:1067503/140‑1 (MQ=255)                    tCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAg                                                                                                        >  1:1190712/1‑140 (MQ=255)                     cAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGc                                                                                                       <  1:98846/140‑1 (MQ=255)                               tACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCATGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGAt                                                                                             <  2:1056894/140‑1 (MQ=255)                                 cGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           >  2:523156/1‑140 (MQ=255)                                 cGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           <  1:523156/140‑1 (MQ=255)                                  gTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCAc                                                                                          <  1:1167751/140‑1 (MQ=255)                                         tctGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           <  1:1181840/132‑1 (MQ=255)                                         tctGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           >  2:1181840/1‑132 (MQ=255)                                         tctGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGaa                                                                                   >  1:40594/1‑140 (MQ=255)                                            ggTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAAc                                                                                >  1:875335/1‑140 (MQ=255)                                             gTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGaaaa                                                                                 <  2:233437/138‑1 (MQ=255)                                             gTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGaaaa                                                                                 >  1:233437/1‑138 (MQ=255)                                             gTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAAcc                                                                               >  1:148065/1‑140 (MQ=255)                                                gTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACg                                                                            <  2:230275/140‑1 (MQ=255)                                                 ttGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACgg                                                                           >  1:490509/1‑140 (MQ=255)                                                 ttGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACg                                                                            <  2:649332/139‑1 (MQ=255)                                                 ttGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACg                                                                            >  1:649332/1‑139 (MQ=255)                                                  tGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACggg                                                                          <  1:219725/140‑1 (MQ=255)                                                  tGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACggg                                                                          <  1:944140/140‑1 (MQ=255)                                                   gCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTTCCCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           >  2:311820/1‑123 (MQ=255)                                                   gCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTTCCCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCa                                                                                           <  1:311820/123‑1 (MQ=255)                                                   gCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGc                                                                         >  2:176715/1‑140 (MQ=255)                                                   gCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGc                                                                         <  2:148065/140‑1 (MQ=255)                                                     aCCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCa                                                                       >  1:206084/1‑140 (MQ=255)                                                         gTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTga                                                                   <  2:206084/140‑1 (MQ=255)                                                          tGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTgag                                                                  >  2:1121428/1‑140 (MQ=255)                                                            cATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGAc                                                                >  2:451449/1‑140 (MQ=255)                                                             aTCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACg                                                               <  1:523177/140‑1 (MQ=255)                                                             aTCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACg                                                               <  2:1190712/140‑1 (MQ=255)                                                              tCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGt                                                              <  1:176715/140‑1 (MQ=255)                                                                 aTCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTgg                                                           >  1:1059937/1‑140 (MQ=255)                                                                 aTCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTgg                                                           >  1:222094/1‑140 (MQ=255)                                                                  tCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGc                                                          <  1:451449/140‑1 (MQ=255)                                                                   cGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTgaga                                                                 >  1:161409/1‑132 (MQ=255)                                                                   cGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTgaga                                                                 <  2:161409/132‑1 (MQ=255)                                                                    gCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCGAGCGAGACGTTGGCaa                                                        >  2:1091201/1‑139 (MQ=255)                                                                    gCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCAt                                                        >  1:155066/1‑140 (MQ=255)                                                                    gCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGG‑CAGTGAGACGTTGGCATa                                                       >  1:744631/1‑140 (MQ=255)                                                                       tCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATaaa                                                     >  1:616390/1‑140 (MQ=255)                                                                           gCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACata                                                 <  1:67446/140‑1 (MQ=255)                                                                                aaTCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATgtgt                                            >  1:366108/1‑140 (MQ=255)                                                                                aaTCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATgtgt                                            >  1:1175088/1‑140 (MQ=255)                                                                                aaTCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATgtgt                                            >  1:752223/1‑140 (MQ=255)                                                                                   cccTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTgtcgt                                         <  2:875335/140‑1 (MQ=255)                                                                                     cTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTgtcgtcg                                       <  2:859054/140‑1 (MQ=255)                                                                                     cTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTGGGCATAAACATATGTgtcgtcg                                       >  1:859054/1‑140 (MQ=255)                                                                                             tt‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGAtt                               >  1:656224/1‑140 (MQ=255)                                                                                             tt‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGAtt                               >  1:347865/1‑140 (MQ=255)                                                                                                  cTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAaca                           <  2:40594/140‑1 (MQ=255)                                                                                                    ggTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAacaca                         <  2:616390/140‑1 (MQ=255)                                                                                                        cGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTgtcgtcgtc                                     <  2:905923/124‑1 (MQ=255)                                                                                                        cGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTgtcgtcgtc                                     >  1:905923/1‑124 (MQ=255)                                                                                                        cGCTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGcc                     >  2:766373/1‑140 (MQ=255)                                                                                                          cTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTg                   >  1:672510/1‑140 (MQ=255)                                                                                                          cTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTg                   >  2:330178/1‑140 (MQ=255)                                                                                                          cTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTg                   >  1:1086761/1‑140 (MQ=255)                                                                                                          cTTTCCAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTg                   >  2:577392/1‑140 (MQ=255)                                                                                                              ccAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGc               <  1:1121428/140‑1 (MQ=255)                                                                                                              ccAGCGGGGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGc               <  2:309222/140‑1 (MQ=255)                                                                                                               cAGCTGTGAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCt              >  1:191075/1‑140 (MQ=255)                                                                                                                   tgtgAAAACAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGcc          <  2:1086761/140‑1 (MQ=255)                                                                                                                       aaaaCAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGa      <  2:1059937/140‑1 (MQ=255)                                                                                                                           cAAAATGAAATGAGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGCCCAGTGAGACGTTGGCATAACCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAg  <  1:1091201/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                TCCACGCGAGCCATCTGCTTCAATGAGAATTACGTCACACTCTGGTCGTTGCACCAGTGCATCAATCGCTTCTGGCGTAAATCCCTGCACTTTT‑CCCTGGTTCGCTTTCCAGCTGTGAAAACAAAATGAAATGGGGGATGTAAGAGACGCATGAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAG  >  NC_000913/3014677‑3014937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑