Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,925	T→C	K237K (AAA→AAG)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,925	0	T	C	100.0%	184.4 / NA	52	K237K (AAA→AAG)	tktA	transketolase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

GCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACG  >  NC_000913/3080789‑3081060                                                                                                                                         |                                                                                                                                        gcgcATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTg                                                                                                                                      <  1:509830/140‑1 (MQ=255)    cATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTgtca                                                                                                                                   >  2:804867/1‑140 (MQ=255)            cAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTTGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgc                                                                                                                           >  1:1393/1‑140 (MQ=255)              gCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcgc                                                                                                                         >  2:1137221/1‑140 (MQ=255)                ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcgcgc                                                                                                                       <  1:804867/140‑1 (MQ=255)                ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcgcgc                                                                                                                       <  1:662856/140‑1 (MQ=255)                ccAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcgcgc                                                                                                                       <  1:611842/140‑1 (MQ=255)                   gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTgt                                                                                                                                     >  2:325175/1‑123 (MQ=255)                   gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTgt                                                                                                                                     <  1:325175/123‑1 (MQ=255)                   gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcg                                                                                                                          >  2:386587/1‑134 (MQ=255)                   gttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTgcg                                                                                                                          <  1:386587/134‑1 (MQ=255)                    ttgttCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGctt                                                                                                                   >  1:867473/1‑140 (MQ=255)                         cgcgGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTtcttct                                                                                                              >  2:954640/1‑140 (MQ=255)                         cgcgGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTtcttct                                                                                                              >  1:434259/1‑140 (MQ=255)                            gggTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACt                                                                                                           <  1:515968/140‑1 (MQ=255)                              gTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTgc                                                                                                         >  2:353305/1‑140 (MQ=255)                               tCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTgcg                                                                                                        <  1:791283/140‑1 (MQ=255)                                  gggCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGt                                                                                                     <  1:274528/140‑1 (MQ=255)                                     cAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTg                                                                                                  <  1:1052549/140‑1 (MQ=255)                                        tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCTCATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCCACTGCGCGTTTGATa                                                                                               >  2:102243/1‑140 (MQ=255)                                        tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATa                                                                                               >  2:261935/1‑140 (MQ=255)                                        tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATa                                                                                               >  2:47035/1‑140 (MQ=255)                                        tttCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATa                                                                                               >  1:52006/1‑140 (MQ=255)                                             gCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGt                                                                                                     >  1:147114/1‑129 (MQ=255)                                             gCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGt                                                                                                     <  2:147114/129‑1 (MQ=255)                                              cGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGcc                                                                                         <  1:933774/140‑1 (MQ=255)                                                tCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCcgc                                                                                       <  1:149263/140‑1 (MQ=255)                                                    ccAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCa                                                                                   <  2:637001/140‑1 (MQ=255)                                                     cAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCAt                                                                                  <  1:261935/140‑1 (MQ=255)                                                             cACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCg                                                                          <  1:1137221/140‑1 (MQ=255)                                                                  tGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTc                                                                     <  1:174008/140‑1 (MQ=255)                                                                     gAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTcgcg                                                                  <  2:867473/140‑1 (MQ=255)                                                                          gTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATaa                                                             >  1:1098680/1‑140 (MQ=255)                                                                             ggTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGt                                                          >  2:747736/1‑140 (MQ=255)                                                                                aCCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGcc                                                       >  1:89697/1‑140 (MQ=255)                                                                                    gCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGcc                                                   >  2:396940/1‑140 (MQ=255)                                                                                    gCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGcc                                                   >  1:614955/1‑140 (MQ=255)                                                                                        tGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTaa                                               >  1:834294/1‑140 (MQ=255)                                                                                                  accgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAAc                                     >  2:938725/1‑140 (MQ=255)                                                                                                          cgaTGATGGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg                             <  2:833704/140‑1 (MQ=255)                                                                                                            atgatgGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt                           <  2:1085086/140‑1 (MQ=255)                                                                                                              gatgGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtgt                         <  1:938725/140‑1 (MQ=255)                                                                                                              gatgGTTTTGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtgt                         <  2:502307/140‑1 (MQ=255)                                                                                                                    tttGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCgg                   <  1:668565/140‑1 (MQ=255)                                                                                                                      tGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTg                 >  1:329334/1‑140 (MQ=255)                                                                                                                      tGCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTg                 >  1:110621/1‑140 (MQ=255)                                                                                                                       gCACATCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGa                >  2:949506/1‑140 (MQ=255)                                                                                                                          caTCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAcc             <  2:1393/140‑1 (MQ=255)                                                                                                                            tCAGCAGGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAg           <  1:806937/140‑1 (MQ=255)                                                                                                                                 agGGAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTc      <  1:396940/140‑1 (MQ=255)                                                                                                                                  gggAAGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCa     >  1:1091775/1‑140 (MQ=255)                                                                                                                                     aaGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACg  >  2:798114/1‑140 (MQ=255)                                                                                                                                     aaGGCTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACg  >  2:886179/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCGCATATTTCCAGCCCAGTTGTTCGCGGGTCAGGGCAATTTCAGCGTCGCCCAGCGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACG  >  NC_000913/3080789‑3081060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑