Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,513	T→C	N467D (AAC→GAC)	speA ←	arginine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,513	0	T	C	100.0%	276.5 / NA	77	N467D (AAC→GAC)	speA	arginine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/42);  total (35/42)

CCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTG  >  NC_000913/3084376‑3084643                                                                                                                                          |                                                                                                                                   cccTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGtctt                                                                                                                                  >  1:944467/1‑140 (MQ=255)  ccTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTg                                                                                                                                 >  1:726305/1‑140 (MQ=255)  ccTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTg                                                                                                                                 >  1:614391/1‑140 (MQ=255)    ttCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGtctt                                                                                                                                  >  1:558680/1‑137 (MQ=255)      ccAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCTATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGa                                                                                                                             >  2:5079/1‑140 (MQ=255)            gCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAgc                                                                                                                       <  2:972919/140‑1 (MQ=255)              aGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAgctg                                                                                                                     >  1:635116/1‑140 (MQ=255)              aGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAgctg                                                                                                                     >  1:449102/1‑140 (MQ=255)                aaCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAgctgct                                                                                                                   >  1:23004/1‑140 (MQ=255)                 aCCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATCCGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCtt                                                                                                                  <  2:841062/140‑1 (MQ=255)                     ggAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGc                                                                                                              <  2:752452/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             <  2:333298/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             <  2:726305/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             <  2:944467/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             <  2:802423/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             <  2:1136584/140‑1 (MQ=255)                      gAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCa                                                                                                             >  2:1157242/1‑140 (MQ=255)                       aacaacTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCAc                                                                                                            <  2:614391/140‑1 (MQ=255)                            cTGGTCGATCCCCCATGCGTCCGGCATCGTCTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCAt                                                                                                       >  2:1145305/1‑140 (MQ=255)                               gTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAATTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGc                                                                                                    >  2:553765/1‑140 (MQ=255)                               gTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGc                                                                                                    >  1:302161/1‑140 (MQ=255)                               gTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGc                                                                                                    >  2:790603/1‑140 (MQ=255)                                tCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGTGGATCCAGCTGCTTTTGCACTTCATGGca                                                                                                   <  1:607697/140‑1 (MQ=255)                                tCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGca                                                                                                   <  1:553765/140‑1 (MQ=255)                                tCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGca                                                                                                   <  2:75310/140‑1 (MQ=255)                                  gATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGcaca                                                                                                 <  2:23004/140‑1 (MQ=255)                                    tCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATg                                                                                               >  2:365262/1‑140 (MQ=255)                                          aTGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGAGGTATAGTGTGTCCGAGATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCaaa                                                                                         >  2:1207883/1‑140 (MQ=255)                                             cGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGTACTTCATGGcaca                                                                                                 <  1:917732/129‑1 (MQ=255)                                             cGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGTACTTCATGGcaca                                                                                                 >  2:917732/1‑129 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGca                                                                                                   >  1:773166/1‑126 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGca                                                                                                   <  2:773166/126‑1 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATaaa                                                                                     >  2:627067/1‑140 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATaaa                                                                                     <  2:204598/140‑1 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATaaa                                                                                     >  1:325345/1‑140 (MQ=255)                                              gTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATaaa                                                                                     >  1:204598/1‑140 (MQ=255)                                               tCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAg                                                                                    >  1:550508/1‑140 (MQ=255)                                               tCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAg                                                                                    >  1:808245/1‑140 (MQ=255)                                               tCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAg                                                                                    >  1:1040138/1‑140 (MQ=255)                                               tCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAg                                                                                    >  1:10790/1‑140 (MQ=255)                                                      tCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCa                                                                             >  1:772819/1‑140 (MQ=255)                                                        gACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGc                                                                           <  1:1027984/140‑1 (MQ=255)                                                        gACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGc                                                                           <  2:876586/140‑1 (MQ=255)                                                             gAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATg                                                                      <  1:1001046/140‑1 (MQ=255)                                                                   cGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGtt                                                                >  1:364548/1‑140 (MQ=255)                                                                     agaAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGc                                                                           <  1:307432/127‑1 (MQ=255)                                                                     agaAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGc                                                                           >  2:307432/1‑127 (MQ=255)                                                                       aaGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTg                                                            <  2:772819/140‑1 (MQ=255)                                                                       aaGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTg                                                            <  1:340975/140‑1 (MQ=255)                                                                       aaGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTg                                                            <  1:1157242/140‑1 (MQ=255)                                                                       aaGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTg                                                            <  2:808245/140‑1 (MQ=255)                                                                                tACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTaaa                                                   >  2:1088449/1‑140 (MQ=255)                                                                                    ttttGTCTGCTATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATg                                               >  2:693154/1‑140 (MQ=255)                                                                                       tGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGTAGGCTAAAGATGCCg                                            >  2:524304/1‑140 (MQ=255)                                                                                       tGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCg                                            >  2:360145/1‑140 (MQ=255)                                                                                       tGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCg                                            <  1:627067/140‑1 (MQ=255)                                                                                           cGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAag                                        >  2:51958/1‑140 (MQ=255)                                                                                           cGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAag                                        >  1:1015948/1‑140 (MQ=255)                                                                                            gCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAaga                                       <  1:360145/140‑1 (MQ=255)                                                                                             ccATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAagag                                      >  2:817034/1‑140 (MQ=255)                                                                                             ccATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAagag                                      <  2:10790/140‑1 (MQ=255)                                                                                             ccATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAagag                                      <  1:790603/140‑1 (MQ=255)                                                                                                tACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAg                                   <  2:1198181/140‑1 (MQ=255)                                                                                                 aCGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGc                                  <  1:693154/140‑1 (MQ=255)                                                                                                  ccttCCTGCCGCTCGTCGATAATCGGACGATGAGCCAGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCCCAAATAAAGCTGCTCAGCCCATGCCCGTTCTTGCCGGCTAAAGATGCCGGAAGAGTAGcc                                 <  2:550508/138‑1 (MQ=255)                                                                                                  cGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGcc                                 <  1:1145305/140‑1 (MQ=255)                                                                                                  cGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGcc                                 <  1:268993/140‑1 (MQ=255)                                                                                                  cGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGcc                                 <  1:1207883/140‑1 (MQ=255)                                                                                                       cTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAg                                   >  1:764723/1‑133 (MQ=255)                                                                                                       cTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAg                                   <  2:764723/133‑1 (MQ=255)                                                                                                       cTGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGatat                            <  1:770168/140‑1 (MQ=255)                                                                                                        tGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGc                                  <  2:37361/133‑1 (MQ=255)                                                                                                        tGCAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGc                                  >  1:37361/1‑133 (MQ=255)                                                                                                          cAGCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGaa                         >  2:918769/1‑140 (MQ=255)                                                                                                            gCTCGTCGATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATg                       <  1:607058/140‑1 (MQ=255)                                                                                                               cgtcgATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGcc                                 <  2:439605/127‑1 (MQ=255)                                                                                                               cgtcgATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGcc                                 >  1:439605/1‑127 (MQ=255)                                                                                                                  cgATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGGAGAGTAGCCGATATGAATGTCGTGc                 <  1:1062534/140‑1 (MQ=255)                                                                                                                  cgATAATCGGACGATGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGc                 <  2:1015948/140‑1 (MQ=255)                                                                                                                                tGAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACt   >  1:680853/1‑140 (MQ=255)                                                                                                                                 gAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTg  <  2:635116/140‑1 (MQ=255)                                                                                                                                 gAGCACGGTCTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTg  <  2:449102/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CCCTTCCAGCGGCAGAACCGGGAACAACTGGTCGATCCCCCATGCGTCCGGCATCGACTGGAACAGCGAGAAGTTGACGTACATTTTGTCCGCCATACGTTCCTGCAGCTCGTCGATAATCGGACGATGAGCACGGTTTTGCGGATCCAGCTGCTTTTGCACTTCATGGCACATGCTCAAATAAAGCTGCTCAGCCCATGCACGTTCTTGCAGGCTAAAGATGCCGGAAGAGTAGCCGATATGAATGTCGTGCAGATCCATCTGACTG  >  NC_000913/3084376‑3084643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑