Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,184	A→G	V261A (GTC→GCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,184	0	A	G	100.0%	165.1 / NA	48	V261A (GTC→GCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

GTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCC  >  NC_000913/3200055‑3200318                                                                                                                                  |                                                                                                                                       gtagagtagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAAt                                                                                                                              >  2:750749/1‑140 (MQ=255)      gtagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTg                                                                                                                         >  2:378377/1‑140 (MQ=255)        agaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGcc                                                                                                                       <  2:34953/140‑1 (MQ=255)               cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGGTCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  2:721262/1‑140 (MQ=255)               cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  1:1197583/1‑140 (MQ=255)               cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCt                                                                                                                >  1:445416/1‑140 (MQ=255)                 tCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGt                                                                                                              >  2:971587/1‑140 (MQ=255)                 tCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGt                                                                                                              >  2:569215/1‑140 (MQ=255)                             ccGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCa                                                                                                  <  2:1197583/140‑1 (MQ=255)                                cAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCg                                                                                               >  2:1139579/1‑140 (MQ=255)                                cAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCg                                                                                               <  1:569215/140‑1 (MQ=255)                                   aaGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTg                                                                                            >  2:27774/1‑140 (MQ=255)                                    aGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGc                                                                                           <  2:982013/140‑1 (MQ=255)                                      cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcg                                                                                         >  2:880041/1‑140 (MQ=255)                                      cAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcg                                                                                         <  1:587041/140‑1 (MQ=255)                                       aaTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgc                                                                                        >  1:1111226/1‑140 (MQ=255)                                        aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  2:119477/1‑140 (MQ=255)                                        aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  2:1177111/1‑140 (MQ=255)                                        aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                                                                       >  1:818642/1‑140 (MQ=255)                                         tGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGt                                                                                      <  1:1139579/140‑1 (MQ=255)                                            tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                   <  1:971587/140‑1 (MQ=255)                                            tCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTAcc                                                                                   <  1:392458/140‑1 (MQ=255)                                              gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                 <  2:445416/140‑1 (MQ=255)                                              gCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCca                                                                                 <  2:860331/140‑1 (MQ=255)                                                cTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCcaca                                                                               <  1:119477/140‑1 (MQ=255)                                                 tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                                                              <  1:750749/140‑1 (MQ=255)                                                 tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                                                              <  1:668953/140‑1 (MQ=255)                                                   gCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGtt                                                                            <  2:1073055/140‑1 (MQ=255)                                                      ttGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttctt                                                                         <  2:720806/140‑1 (MQ=255)                                                               cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttcttc                                                                        >  2:6892/1‑132 (MQ=255)                                                               cagATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGttcttc                                                                        <  1:6892/132‑1 (MQ=255)                                                                               gtgCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGc                                                >  1:174075/1‑140 (MQ=255)                                                                                   tCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCTACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGc                                            >  2:489451/1‑140 (MQ=255)                                                                                   tCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGc                                            >  1:74515/1‑140 (MQ=255)                                                                                        ttACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAg                                       >  1:677649/1‑140 (MQ=255)                                                                                           cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                    <  1:721262/140‑1 (MQ=255)                                                                                           cgcgGCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAgcgc                                    <  1:126634/140‑1 (MQ=255)                                                                                               gCACAATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCt                                >  2:385171/1‑140 (MQ=255)                                                                                                 acaATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTc                              >  1:12092/1‑140 (MQ=255)                                                                                                 acaATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTc                              >  2:225772/1‑140 (MQ=255)                                                                                                  caATACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTcc                             <  2:1111226/140‑1 (MQ=255)                                                                                                     tACCACCGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGc                          <  1:1177111/140‑1 (MQ=255)                                                                                                        caccGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGcccc                       <  2:174075/140‑1 (MQ=255)                                                                                                          ccGAACAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCTCAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTg                     >  2:560678/1‑140 (MQ=255)                                                                                                              aCAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTcc                 >  1:829166/1‑140 (MQ=255)                                                                                                               cAGCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCca                >  2:285623/1‑140 (MQ=255)                                                                                                                 gCATCAGCGTATGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCcaca              <  1:385171/140‑1 (MQ=255)                                                                                                                           aTGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTg    <  2:12092/140‑1 (MQ=255)                                                                                                                            tGACGGGCCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCT‑ACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGcc  >  1:155825/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       GTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTCGAGCGCCGCTTCCAGCCCCTGTTCCACATCGGCTTTTGCC  >  NC_000913/3200055‑3200318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑