Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,593	A→G	T438A (ACC→GCC)	garD →	galactarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,593	0	A	G	100.0%	172.4 / NA	49	T438A (ACC→GCC)	garD	galactarate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

AATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACT  >  NC_000913/3276463‑3276724                                                                                                                                   |                                                                                                                                    aaTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTg                                                                                                                            <  2:1050934/140‑1 (MQ=255) aaTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTg                                                                                                                            >  1:950259/1‑140 (MQ=255)   ttgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGt                                                                                                                          >  1:1106914/1‑140 (MQ=255)    tgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTa                                                                                                                         <  2:482998/140‑1 (MQ=255)    tgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTa                                                                                                                         <  1:594871/140‑1 (MQ=255)     gttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTAc                                                                                                                        <  2:1187487/140‑1 (MQ=255)      ttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACg                                                                                                                       >  2:854318/1‑140 (MQ=255)             gCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACg                                                                                                                >  1:1095752/1‑140 (MQ=255)              cTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACgg                                                                                                               >  2:1133636/1‑140 (MQ=255)                 tCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCt                                                                                                            <  2:272771/140‑1 (MQ=255)                 tCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCt                                                                                                            >  2:185240/1‑140 (MQ=255)                    cccGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGAt                                                                                                         >  1:314803/1‑140 (MQ=255)                      cGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATgg                                                                                                       >  2:439331/1‑140 (MQ=255)                        gTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCg                                                                                                     >  2:1103229/1‑140 (MQ=255)                           aaCGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTa                                                                                                  >  1:760677/1‑140 (MQ=255)                            aCGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTAc                                                                                                 <  1:493171/140‑1 (MQ=255)                             cGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTAcc                                                                                                <  2:80550/140‑1 (MQ=255)                                            ggATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGc                                                                                 <  2:314803/140‑1 (MQ=255)                                            ggATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGc                                                                                 <  1:151680/140‑1 (MQ=255)                                                   tttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGc                                                                          >  1:780962/1‑140 (MQ=255)                                                   tttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGc                                                                          <  2:1095752/140‑1 (MQ=255)                                                    ttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCa                                                                         <  2:950259/140‑1 (MQ=255)                                                          cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:939712/1‑140 (MQ=255)                                                          cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:276537/1‑140 (MQ=255)                                                          cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:245317/1‑140 (MQ=255)                                                            cgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTg                                                                 >  2:547282/1‑140 (MQ=255)                                                            cgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTg                                                                 >  2:272560/1‑140 (MQ=255)                                                                gccagccaGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGa                                                             >  1:824439/1‑140 (MQ=255)                                                                    gccagcGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCg                                                         <  2:824439/140‑1 (MQ=255)                                                                     ccagcGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGc                                                        <  2:1106914/140‑1 (MQ=255)                                                                         cGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGt                                                    >  1:1201056/1‑140 (MQ=255)                                                                             tttGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGa                                                <  1:854318/140‑1 (MQ=255)                                                                                 tCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                                            <  2:760677/140‑1 (MQ=255)                                                                                 tCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                                            <  2:457178/140‑1 (MQ=255)                                                                                   tgtgGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAAt                                          <  1:1103229/140‑1 (MQ=255)                                                                                   tgtgGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAAt                                          <  1:185240/140‑1 (MQ=255)                                                                                                  gTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           <  1:547282/140‑1 (MQ=255)                                                                                                    ggCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCa                         <  2:1201056/140‑1 (MQ=255)                                                                                                       ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGTCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       >  2:1136317/1‑139 (MQ=255)                                                                                                       ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       <  1:224240/139‑1 (MQ=255)                                                                                                       ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       >  2:224240/1‑139 (MQ=255)                                                                                                       ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       <  1:1136317/139‑1 (MQ=255)                                                                                                       ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCa                      >  2:1211517/1‑140 (MQ=255)                                                                                                         cGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATc                    >  2:1156864/1‑140 (MQ=255)                                                                                                                  caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           >  1:1185807/1‑124 (MQ=255)                                                                                                                  caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           <  2:1185807/124‑1 (MQ=255)                                                                                                                  caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGc           <  1:1156864/140‑1 (MQ=255)                                                                                                                     tGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGCACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCgaa        >  2:731663/1‑140 (MQ=255)                                                                                                                       cAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCgaaga      <  1:245317/140‑1 (MQ=255)                                                                                                                         aGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGaaa    <  1:731663/140‑1 (MQ=255)                                                                                                                           tgtTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACt  <  2:416742/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    AATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACT  >  NC_000913/3276463‑3276724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑