Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,467,351	A→G	F835L (TTC→CTC)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,467,351	0	A	G	100.0%	306.4 / NA	85	F835L (TTC→CTC)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/38);  total (47/38)

CTTTAGTCAGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAACACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGCACACTTA  >  NC_000913/3467216‑3467487                                                                                                                                        |                                                                                                                                         cTTTAGTCAGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCAcc                                                                                                                                      >  1:192625/1‑140 (MQ=255)       tCAGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGccc                                                                                                                                >  2:679525/1‑140 (MQ=255)         aGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTg                                                                                                                              >  2:1161119/1‑140 (MQ=255)         aGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTg                                                                                                                              >  1:955574/1‑140 (MQ=255)          ggCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGa                                                                                                                             >  2:850227/1‑140 (MQ=255)           gCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGaa                                                                                                                            >  2:379843/1‑140 (MQ=255)           gCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGaa                                                                                                                            >  2:1205393/1‑140 (MQ=255)                  aaTTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGtt                                                                                                                     <  2:1150103/140‑1 (MQ=255)                     tCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCAc                                                                                                                  >  1:372701/1‑140 (MQ=255)                            aCAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAAcc                                                                                                           >  1:1060168/1‑140 (MQ=255)                                gTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       >  1:1161460/1‑140 (MQ=255)                                gTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       <  2:591035/140‑1 (MQ=255)                                gTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       <  1:1205393/140‑1 (MQ=255)                                gTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACAAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       >  2:676828/1‑140 (MQ=255)                                   aGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    <  1:472530/140‑1 (MQ=255)                                   aGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    <  2:192625/140‑1 (MQ=255)                                   aGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    <  1:379843/140‑1 (MQ=255)                                   aGTGCCGCCCGGCAGGTCGCGCGCGACTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    <  1:850227/140‑1 (MQ=255)                                    gTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGt                                                                                                   <  2:1161460/140‑1 (MQ=255)                                     tGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCAt                                                                                                         >  1:337695/1‑133 (MQ=255)                                     tGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCAt                                                                                                         <  2:337695/133‑1 (MQ=255)                                     tGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTg                                                                                                  <  1:679525/140‑1 (MQ=255)                                        cgccCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCAt                                                                                                         <  1:393069/130‑1 (MQ=255)                                        cgccCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCAt                                                                                                         >  2:393069/1‑130 (MQ=255)                                          cccagCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTAcc                                                                                             >  2:779523/5‑140 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATTACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTAcc                                                                                             >  2:923070/1‑140 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       <  2:709109/130‑1 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       >  1:709109/1‑130 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    <  1:727702/133‑1 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCg                                                                                                    >  2:727702/1‑133 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTg                                                                                                  <  1:952644/135‑1 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTg                                                                                                  >  2:952644/1‑135 (MQ=255)                                          cccGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTAcc                                                                                             >  2:850758/1‑140 (MQ=255)                                             ggCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCcat                                                                                          <  2:372701/140‑1 (MQ=255)                                              gCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       >  2:322912/1‑126 (MQ=255)                                              gCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCg                                                                                                       <  1:322912/126‑1 (MQ=255)                                               cAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCcatca                                                                                        <  1:779523/140‑1 (MQ=255)                                                     gcgcgcgATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGt                                                                                  >  1:387437/1‑140 (MQ=255)                                                      cgcgcgATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTc                                                                                 <  1:172887/140‑1 (MQ=255)                                                        cgcgATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCaa                                                                               >  2:53614/1‑140 (MQ=255)                                                         gcgATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATAATAGCGTCAAt                                                                              <  1:375672/140‑1 (MQ=255)                                                            aTTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTc                                                                           >  2:100164/1‑140 (MQ=255)                                                                 ccAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATTACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCAt                                                                      <  1:923070/140‑1 (MQ=255)                                                                   aGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCg                                                                    <  2:999316/140‑1 (MQ=255)                                                                   aGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCg                                                                    <  2:387437/140‑1 (MQ=255)                                                                   aGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCg                                                                    <  1:49510/140‑1 (MQ=255)                                                                    gATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGc                                                                   <  1:767995/140‑1 (MQ=255)                                                                       accaaccaTACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGctct                                                                >  1:561831/1‑140 (MQ=255)                                                                            ccaTACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTg                                                           >  1:780843/1‑140 (MQ=255)                                                                               tACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCAt                                                                      >  2:700512/1‑126 (MQ=255)                                                                               tACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCAt                                                                      <  1:700512/126‑1 (MQ=255)                                                                               tACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTgg                                                          >  1:662586/1‑138 (MQ=255)                                                                               tACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTgg                                                          <  2:662586/138‑1 (MQ=255)                                                                               tACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTAGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTACATCGCTCTTATTGGGa                                                        >  2:420886/1‑140 (MQ=255)                                                                               tACCGAGATTACGCTTCTGAGCATCCTGCATGACCAGACGGGCATCAGAGAGATAGAGCACCTCGCCCTGAACGACGTTCCCGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGa                                                        >  2:1162834/1‑140 (MQ=255)                                                                                aCCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGAt                                                       >  1:97578/1‑140 (MQ=255)                                                                                aCCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGAt                                                       >  2:402521/1‑140 (MQ=255)                                                                                 ccGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATg                                                      <  2:780843/140‑1 (MQ=255)                                                                                  cGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTa                                                              <  1:1216470/131‑1 (MQ=255)                                                                                  cGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTa                                                              >  2:1216470/1‑131 (MQ=255)                                                                                      aTTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGc                                                 >  2:895643/1‑140 (MQ=255)                                                                                       ttACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGcc                                                >  2:368109/1‑140 (MQ=255)                                                                                       ttACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGcc                                                >  2:485781/1‑140 (MQ=255)                                                                                          cGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATgg                                                     >  2:713893/1‑132 (MQ=255)                                                                                          cGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATgg                                                     <  1:713893/132‑1 (MQ=255)                                                                                            cTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCa                                           <  2:203885/140‑1 (MQ=255)                                                                                            cTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCACTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCa                                           <  2:1060168/140‑1 (MQ=255)                                                                                                tGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATtg                                       >  2:1203079/1‑140 (MQ=255)                                                                                                      tCCTGCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATg                                 <  2:885427/140‑1 (MQ=255)                                                                                                          gCATGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAgg                             >  1:409355/1‑140 (MQ=255)                                                                                                            aTGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGtt                           >  2:112050/1‑140 (MQ=255)                                                                                                            aTGACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGtt                           <  1:527843/140‑1 (MQ=255)                                                                                                              gACCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTgg                         <  1:676828/140‑1 (MQ=255)                                                                                                               aCCAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGc                        >  2:728611/1‑140 (MQ=255)                                                                                                                 cAGACGAGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCaa                      >  1:663168/1‑140 (MQ=255)                                                                                                                       aGCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCCTTCAATTGTGAATGCAGGTTGGCAATCGCAg                <  2:561831/140‑1 (MQ=255)                                                                                                                        gCATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGa               >  1:586485/1‑140 (MQ=255)                                                                                                                         cATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGAc              >  1:569431/1‑140 (MQ=255)                                                                                                                         cATCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGAc              >  1:281842/1‑140 (MQ=255)                                                                                                                          aTCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACg             <  2:955574/140‑1 (MQ=255)                                                                                                                           tCAGAGAGATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGt            >  2:1024788/1‑140 (MQ=255)                                                                                                                             agagagATAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGttc          >  1:887991/1‑139 (MQ=255)                                                                                                                              gagagaTAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGa               <  1:909255/134‑1 (MQ=255)                                                                                                                              gagagaTAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGa               >  2:909255/1‑134 (MQ=255)                                                                                                                              gagagaTAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGc         <  2:374360/140‑1 (MQ=255)                                                                                                                                 agaTAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGcaca      >  1:907284/1‑140 (MQ=255)                                                                                                                                   aTAGAGCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGCACACt    >  2:261875/1‑140 (MQ=255)                                                                                                                                     agagCACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGCACACTTa  <  2:281842/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CTTTAGTCAGGCCGTGGAATTCCGGAGACAGGTTAGTGCCGCCCGGCAGGTCGCGCGCGATTGACCAGATACCAACCATACCGAGATTACGCTTCTGCGCATCCTGCATGACCAGACGAGCATCAGAGAGATAGAACACCTCGCCCTGAACGTCGTTCACGCCAACCATCGGCGTGGTACCCATCATAGCGTCAATTTCTGCATCGCTCTTATTGGGATGGAGGCCTTTCAATTGTGAATGCAGGTTGGCAATCGCAGACGTTGCACACTTA  >  NC_000913/3467216‑3467487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑