Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,141	G→A	S213S (TCG→TCA)	proA →	glutamate‑5‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,141	0	G	A	100.0%	237.3 / NA	67	S213S (TCG→TCA)	proA	glutamate‑5‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/35);  total (32/35)

CGGCGGGTGCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTG  >  NC_000913/262008‑262271                                                                                                                                      |                                                                                                                                   cggcggGTGCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATc                                                                                                                              <  2:1137737/140‑1 (MQ=255)     gggTGCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCgg                                                                                                                          <  1:284727/140‑1 (MQ=255)         gCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGAt                                                                                                                      <  1:478770/140‑1 (MQ=255)          ccGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATc                                                                                                                     <  1:326699/140‑1 (MQ=255)           cGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATca                                                                                                                    <  1:164476/140‑1 (MQ=255)              gCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAg                                                                                                                 <  1:556354/140‑1 (MQ=255)               cAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAgg                                                                                                                <  2:155703/140‑1 (MQ=255)               cAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAgg                                                                                                                <  1:497849/140‑1 (MQ=255)               cAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAgg                                                                                                                <  1:335998/140‑1 (MQ=255)                          tAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCg                                                                                                     <  1:447760/140‑1 (MQ=255)                                tGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGcc                                                                                               >  1:695213/1‑140 (MQ=255)                                tGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGcc                                                                                               >  1:329204/1‑140 (MQ=255)                                tGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGcc                                                                                               >  1:353721/1‑140 (MQ=255)                                 gACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCa                                                                                              >  1:979892/1‑140 (MQ=255)                                      tGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATAttt                                                                                         >  2:1111342/1‑140 (MQ=255)                                      tGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATAttt                                                                                         >  1:195366/1‑140 (MQ=255)                                       gcgcTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTa                                                                                        >  2:884459/1‑140 (MQ=255)                                        cgcTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTAc                                                                                       >  1:1094508/1‑140 (MQ=255)                                            ggTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTtg                                                                                   >  1:686877/1‑140 (MQ=255)                                             gTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTtga                                                                                  >  2:521966/1‑140 (MQ=255)                                                aGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTtgatga                                                                               <  1:1111342/140‑1 (MQ=255)                                                aGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTtgatga                                                                               <  1:22746/140‑1 (MQ=255)                                                aGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTtgatga                                                                               <  1:372342/140‑1 (MQ=255)                                                  tGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGaaa                                                                             <  2:695213/140‑1 (MQ=255)                                                         ctgcGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTaga                                                                      <  1:578737/140‑1 (MQ=255)                                                                      aaTACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCa                                                         <  1:160123/140‑1 (MQ=255)                                                                      aaTACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATC‑CAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCAt                                                        >  2:421173/1‑140 (MQ=255)                                                                          cATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTaa                                                     <  2:195366/140‑1 (MQ=255)                                                                            tCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTaaaa                                                   >  2:1008437/1‑140 (MQ=255)                                                                            tCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTaaaa                                                   >  1:134418/1‑140 (MQ=255)                                                                             cGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAg                                                  >  2:975467/1‑140 (MQ=255)                                                                               aCATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTg                                                >  2:825557/1‑140 (MQ=255)                                                                               aCATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTg                                                >  1:458804/1‑140 (MQ=255)                                                                                cATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGa                                               >  2:695736/1‑140 (MQ=255)                                                                                     tGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTc                                          <  1:975467/140‑1 (MQ=255)                                                                                     tGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTc                                          >  1:261055/1‑140 (MQ=255)                                                                                            gcgTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGa                                   >  2:972145/1‑140 (MQ=255)                                                                                             cgTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGaa                                  >  2:754191/1‑140 (MQ=255)                                                                                             cgTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGaa                                  >  2:1193232/1‑140 (MQ=255)                                                                                             cgTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGaa                                  >  1:595854/1‑140 (MQ=255)                                                                                                 gtggCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACt                              >  2:1008908/1‑140 (MQ=255)                                                                                                  tggCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAAcg                                      >  1:464452/1‑131 (MQ=255)                                                                                                  tggCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAAcg                                      <  2:464452/131‑1 (MQ=255)                                                                                                   ggCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCa                            <  1:825557/140‑1 (MQ=255)                                                                                                      gcTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCg                         >  2:127782/1‑140 (MQ=255)                                                                                                       cTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGt                        >  1:463175/1‑140 (MQ=255)                                                                                                       cTGGTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGt                        <  2:595854/140‑1 (MQ=255)                                                                                                          gTTTGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATC‑CAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGa                    <  1:421173/140‑1 (MQ=255)                                                                                                            ttGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAg                   <  2:137539/140‑1 (MQ=255)                                                                                                            ttGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAg                   <  1:127782/140‑1 (MQ=255)                                                                                                             tGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  1:327319/140‑1 (MQ=255)                                                                                                             tGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  2:261055/140‑1 (MQ=255)                                                                                                             tGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  1:1193232/140‑1 (MQ=255)                                                                                                             tGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  2:686877/140‑1 (MQ=255)                                                                                                             tGCATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGACGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  1:63676/140‑1 (MQ=255)                                                                                                               cATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGca                 >  1:963709/1‑139 (MQ=255)                                                                                                               cATAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGca                 <  2:963709/139‑1 (MQ=255)                                                                                                                aTAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGcaca               <  2:1009383/140‑1 (MQ=255)                                                                                                                aTAAACTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGcaca               <  2:458804/140‑1 (MQ=255)                                                                                                                    aCTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  >  2:494995/1‑133 (MQ=255)                                                                                                                    aCTGTGCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGc                  <  1:494995/133‑1 (MQ=255)                                                                                                                       gtgCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATa        <  2:353721/140‑1 (MQ=255)                                                                                                                         gCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTc                       >  2:504068/1‑123 (MQ=255)                                                                                                                         gCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTc                       <  1:504068/123‑1 (MQ=255)                                                                                                                         gCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACg      >  1:9521/1‑140 (MQ=255)                                                                                                                         gCCGTGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACg      >  1:319873/1‑140 (MQ=255)                                                                                                                             tGAACAGTCAACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTg  >  1:620633/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   CGGCGGGTGCCGTGCAGGCGATTGATAATCCTGACCGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGTGGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGTGGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAAGTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTG  >  NC_000913/262008‑262271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑