Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,633	T→C	intergenic (‑41/+98)	gntK ← / ← gntR	D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,633	0	T	C	100.0%	205.7 / NA	57	intergenic (‑41/+98)	gntK/gntR	D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/33);  total (24/33)

TGATGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAA  >  NC_000913/3577501‑3577767                                                                                                                                     |                                                                                                                                       tGATGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTTGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGtt                                                                                                                                 <  1:814364/140‑1 (MQ=255) tGATGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGtt                                                                                                                                 <  1:640047/140‑1 (MQ=255)  gATGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTa                                                                                                                                <  2:72172/140‑1 (MQ=255)   aTGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTAt                                                                                                                               <  1:865980/140‑1 (MQ=255)        ccACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACATTACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTa                                                                                                                          >  2:383118/1‑140 (MQ=255)        ccACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGtt                                                                                                                                 <  2:920316/133‑1 (MQ=255)        ccACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGtt                                                                                                                                 >  1:920316/1‑133 (MQ=255)        ccACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTa                                                                                                                          >  2:599703/1‑140 (MQ=255)              cACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTg                                                                                                                    <  1:1004054/140‑1 (MQ=255)                   gCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAg                                                                                                               <  1:74613/140‑1 (MQ=255)                   gCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAg                                                                                                               <  1:51779/140‑1 (MQ=255)                       ccGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACATTACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCgg                                                                                                           <  1:383118/140‑1 (MQ=255)                       ccGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCgg                                                                                                           <  1:599703/140‑1 (MQ=255)                        cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGa                                                                                                          >  1:1164522/1‑140 (MQ=255)                        cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGa                                                                                                          <  1:452751/140‑1 (MQ=255)                                tGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCa                                                                                                  <  1:585517/140‑1 (MQ=255)                                 gCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAg                                                                                                 <  2:471933/140‑1 (MQ=255)                                    gCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                              <  1:112600/140‑1 (MQ=255)                                                  ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatccatc                                                                                   >  2:1017945/1‑137 (MQ=255)                                                  ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatccatc                                                                                   <  1:1017945/137‑1 (MQ=255)                                                     tCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGcc                                                                             >  2:34022/1‑140 (MQ=255)                                                       aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                           <  1:34022/140‑1 (MQ=255)                                                       aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                           <  1:715323/140‑1 (MQ=255)                                                       aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                           <  1:245346/140‑1 (MQ=255)                                                                     gtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGtat                                                                   <  1:766777/134‑1 (MQ=255)                                                                     gtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGtat                                                                   >  2:766777/1‑134 (MQ=255)                                                                        aTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                           <  2:616765/123‑1 (MQ=255)                                                                        aTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                           >  1:616765/1‑123 (MQ=255)                                                                           aTGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTa                                                       >  2:764163/1‑140 (MQ=255)                                                                                tAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCa                                                  >  2:976169/1‑140 (MQ=255)                                                                                tAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCa                                                  <  1:1054586/140‑1 (MQ=255)                                                                                  gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgtg                                                              >  1:937610/1‑126 (MQ=255)                                                                                  gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgtg                                                              <  2:937610/126‑1 (MQ=255)                                                                                  gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCaaa                                                >  2:752745/1‑140 (MQ=255)                                                                                   tCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAt                                               >  2:1166431/1‑140 (MQ=255)                                                                                   tCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAt                                               >  2:417884/1‑140 (MQ=255)                                                                                      tGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtg                                            <  2:1020964/140‑1 (MQ=255)                                                                                        cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                          >  2:1025823/1‑140 (MQ=255)                                                                                          cAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTggg                                        <  2:1006821/140‑1 (MQ=255)                                                                                           aaaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                       <  1:976169/140‑1 (MQ=255)                                                                                             aGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCtt                                     >  1:104371/1‑140 (MQ=255)                                                                                                 gtgCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                       >  2:267603/1‑134 (MQ=255)                                                                                                 gtgCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                       <  1:267603/134‑1 (MQ=255)                                                                                                  tgCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATa                                <  2:1164522/140‑1 (MQ=255)                                                                                                    cccccACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGa                              >  2:1040895/1‑140 (MQ=255)                                                                                                      cccACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATc                            <  1:417884/140‑1 (MQ=255)                                                                                                      cccACAATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATc                            <  1:361426/140‑1 (MQ=255)                                                                                                          caATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                                  >  1:1135177/1‑130 (MQ=255)                                                                                                          caATACAAGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                                  <  2:1135177/130‑1 (MQ=255)                                                                                                                 aGAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGAc                 <  2:104371/140‑1 (MQ=255)                                                                                                                  gAATTAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACa                >  1:567909/1‑140 (MQ=255)                                                                                                                     ttAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAgg             >  2:590160/1‑140 (MQ=255)                                                                                                                      tAAAAATGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt            >  2:739033/1‑140 (MQ=255)                                                                                                                           aTGTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAAc       <  2:706479/140‑1 (MQ=255)                                                                                                                             gTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCt     <  1:590160/140‑1 (MQ=255)                                                                                                                             gTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCt     <  1:1166431/140‑1 (MQ=255)                                                                                                                             gTTACGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCt     >  1:780289/1‑140 (MQ=255)                                                                                                                                aCGGGCAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTaaa  >  2:849402/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TGATGCGCCACTTCACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAA  >  NC_000913/3577501‑3577767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑