Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,590,214	A→G	V233A (GTG→GCG)	ugpA ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,590,214	0	A	G	100.0%	201.4 / NA	57	V233A (GTG→GCG)	ugpA	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

ATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAA  >  NC_000913/3590079‑3590350                                                                                                                                        |                                                                                                                                           aTGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCgg                                                                                                                                        >  1:614761/1‑140 (MQ=255)  tGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCggg                                                                                                                                       <  1:885673/140‑1 (MQ=255)            cATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCga                                                                                                                             >  2:550942/1‑140 (MQ=255)              tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           <  1:864096/140‑1 (MQ=255)              tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           <  1:578804/140‑1 (MQ=255)              tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           <  2:686138/140‑1 (MQ=255)              tCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           >  1:395882/1‑140 (MQ=255)               cAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaaga                                                                                                                          <  1:588759/140‑1 (MQ=255)                    ccaccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCa                                                                                                                     <  1:438490/140‑1 (MQ=255)                     caccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCAt                                                                                                                    >  1:627452/1‑140 (MQ=255)                        cGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATaca                                                                                                                 >  2:134858/1‑140 (MQ=255)                        cGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATaca                                                                                                                 <  2:627452/140‑1 (MQ=255)                         gACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATacac                                                                                                                >  2:18428/1‑140 (MQ=255)                          aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           <  1:1049505/128‑1 (MQ=255)                          aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCgaag                                                                                                                           >  2:1049505/1‑128 (MQ=255)                          aCTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACAcc                                                                                                               >  2:700939/1‑140 (MQ=255)                            tgtgCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGATGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAg                                                                                                             <  2:799840/140‑1 (MQ=255)                             gtgCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAgg                                                                                                            >  2:261099/1‑140 (MQ=255)                                   cAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACt                                                                                                    <  1:134858/140‑1 (MQ=255)                                                      tCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCa                                                                                 >  1:742803/1‑140 (MQ=255)                                                      tCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCa                                                                                 >  1:1039705/1‑140 (MQ=255)                                                         ggTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCg                                                                              <  1:261099/140‑1 (MQ=255)                                                          gTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCgg                                                                             <  2:742803/140‑1 (MQ=255)                                                           tAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCggg                                                                            >  2:207659/1‑140 (MQ=255)                                                            aaaaCCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCgggg                                                                           <  1:771560/140‑1 (MQ=255)                                                                   tCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAg                                                                    >  1:27822/1‑140 (MQ=255)                                                                    cgcgGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGc                                                                   >  1:211437/1‑140 (MQ=255)                                                                           gATCTTATAAATCATCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                            <  2:910000/140‑1 (MQ=255)                                                                           gATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                            <  1:648701/140‑1 (MQ=255)                                                                            aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                           <  1:1118671/140‑1 (MQ=255)                                                                            aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                           <  2:593664/140‑1 (MQ=255)                                                                            aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                           <  2:395882/140‑1 (MQ=255)                                                                            aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                           <  2:1177121/140‑1 (MQ=255)                                                                            aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                           <  2:1035833/140‑1 (MQ=255)                                                                             tCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                            <  2:646758/140‑1 (MQ=255)                                                                                         gCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAaagaag                                              >  1:69674/1‑140 (MQ=255)                                                                                         gCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAaagaag                                              >  2:22022/1‑140 (MQ=255)                                                                                          cgtcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                             >  2:129820/1‑140 (MQ=255)                                                                                          cgtcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                             >  1:572276/1‑140 (MQ=255)                                                                                            tcgtGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcgg                                           <  2:890945/140‑1 (MQ=255)                                                                                                 gtggCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                                      <  1:645455/140‑1 (MQ=255)                                                                                                       tGAACCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGccc                                >  1:321058/1‑140 (MQ=255)                                                                                                         aaCCGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTg                              >  2:871616/1‑140 (MQ=255)                                                                                                           ccGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCa                            <  1:263014/140‑1 (MQ=255)                                                                                                           ccGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCa                            <  2:572276/140‑1 (MQ=255)                                                                                                           ccGGCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCa                            <  2:614761/140‑1 (MQ=255)                                                                                                              gCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg                         >  1:846471/1‑140 (MQ=255)                                                                                                              gCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg                         >  1:690627/1‑140 (MQ=255)                                                                                                              gCCCGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg                         >  2:168503/1‑140 (MQ=255)                                                                                                              gCCCGCCGGACGTGGCGGCGGCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCg                         >  2:700651/1‑140 (MQ=255)                                                                                                               cccGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGt                        >  2:100119/1‑140 (MQ=255)                                                                                                               cccGCCGGACGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGt                        >  1:842127/1‑140 (MQ=255)                                                                                                                        cGTGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGAT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTg               >  2:184025/1‑140 (MQ=255)                                                                                                                          tGGCGGCGTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCg             <  1:380165/140‑1 (MQ=255)                                                                                                                            gcggcgTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGc           >  1:763549/1‑140 (MQ=255)                                                                                                                              ggcgTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCtt         <  1:700651/140‑1 (MQ=255)                                                                                                                              ggcgTCGATCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCtt         <  2:846471/140‑1 (MQ=255)                                                                                                                                     aTCGCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCaa  <  2:1027961/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                           ATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTT‑‑CACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAA  >  NC_000913/3590079‑3590350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑