Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,616,756	T→C	L192P (CTG→CCG)	nikC →	Ni(2(+)) ABC transporter membrane subunit NikC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,616,756	0	T	C	100.0%	305.2 / NA	86	L192P (CTG→CCG)	nikC	Ni(2(+)) ABC transporter membrane subunit NikC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/51);  total (35/51)

TGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGAC  >  NC_000913/3616623‑3616891                                                                                                                                      |                                                                                                                                        tGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCcgctggt                                                                                                                                   >  2:797001/1‑140 (MQ=255)  ggTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCcgctggtg                                                                                                                                  >  1:622043/1‑140 (MQ=255)  ggTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCcgctggtg                                                                                                                                  >  2:236444/1‑140 (MQ=255)   gTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCcgctggtgc                                                                                                                                 >  1:142566/1‑140 (MQ=255)       gCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGc                                                                                                                             >  2:63970/1‑140 (MQ=255)        cACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCa                                                                                                                            <  2:796786/140‑1 (MQ=255)        cACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCa                                                                                                                            <  2:544674/140‑1 (MQ=255)        cACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCa                                                                                                                            <  2:706849/140‑1 (MQ=255)             aTGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCa                                                                                                                            <  2:139148/135‑1 (MQ=255)             aTGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCa                                                                                                                            >  1:139148/1‑135 (MQ=255)             aTGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACg                                                                                                                         <  1:791701/138‑1 (MQ=255)             aTGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACg                                                                                                                         >  2:791701/1‑138 (MQ=255)              tGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCt                                                                                                                       <  1:1088225/139‑1 (MQ=255)              tGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTg                                                                                                                      >  2:764480/1‑140 (MQ=255)              tGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTg                                                                                                                      >  2:1170702/1‑140 (MQ=255)              tGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGAGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCt                                                                                                                       >  2:1088225/1‑139 (MQ=255)                gTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGCGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGa                                                                                                                    >  1:311454/1‑140 (MQ=255)                  gcgcAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGata                                                                                                                  <  2:286692/140‑1 (MQ=255)                  gcgcAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGata                                                                                                                  <  1:797001/140‑1 (MQ=255)                   cgcAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGatat                                                                                                                 <  1:163719/140‑1 (MQ=255)                   cgcAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGatat                                                                                                                 >  1:195733/1‑140 (MQ=255)                     cAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCg                                                                                                               >  1:1165514/1‑140 (MQ=255)                        ccTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGcc                                                                                                            >  2:5275/1‑140 (MQ=255)                        ccTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGcc                                                                                                            <  1:236444/140‑1 (MQ=255)                        ccTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGcc                                                                                                            >  2:996615/1‑140 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGTCGCGGTGAGCCCTTCGCCGCTGGTGCTGGCAACGCTAGATATCGGCCata                                                                                                         <  1:301812/140‑1 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCata                                                                                                         <  1:409605/140‑1 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCata                                                                                                         >  1:130695/1‑140 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCata                                                                                                         >  2:856260/1‑140 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCata                                                                                                         >  2:374842/1‑140 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCa                                                                                                           <  2:1105674/138‑1 (MQ=255)                           ggTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCa                                                                                                           >  1:1105674/1‑138 (MQ=255)                                  tCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGAtgct                                                                                                  >  1:1122099/1‑140 (MQ=255)                                  tCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGAtgct                                                                                                  >  1:629082/1‑140 (MQ=255)                                  tCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGAtgct                                                                                                  >  1:704956/1‑140 (MQ=255)                                     cTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCa                                                                                               <  1:585574/140‑1 (MQ=255)                                     cTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCa                                                                                               <  2:619215/140‑1 (MQ=255)                                     cTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCa                                                                                               <  2:142566/140‑1 (MQ=255)                                       aCGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACg                                                                                             <  1:1170702/140‑1 (MQ=255)                                        cGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGt                                                                                            >  2:511315/1‑140 (MQ=255)                                          ccAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTcg                                                                                          >  1:195964/1‑140 (MQ=255)                                              cgcgAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCggg                                                                                      <  1:880463/140‑1 (MQ=255)                                               gcgAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCgggg                                                                                     <  1:511315/140‑1 (MQ=255)                                               gcgAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCgggg                                                                                     <  2:1165514/140‑1 (MQ=255)                                               gcgAGTTTGTGCTGGCGTCACGGCTTCCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCgggg                                                                                     <  2:1190332/140‑1 (MQ=255)                                                 gAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGAt                                                                                   <  1:374842/140‑1 (MQ=255)                                                    tttGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTc                                                                                <  2:1122624/140‑1 (MQ=255)                                                    tttGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTc                                                                                <  1:959391/140‑1 (MQ=255)                                                     ttGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCt                                                                               <  1:996615/140‑1 (MQ=255)                                                        tgCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCggg                                                                                      >  1:1168343/1‑130 (MQ=255)                                                        tgCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCggg                                                                                      <  2:1168343/130‑1 (MQ=255)                                                          cTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCt                                                                          <  2:1122099/140‑1 (MQ=255)                                                          cTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCt                                                                          <  1:148641/140‑1 (MQ=255)                                                              cGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGGGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGc                                                                      <  1:306290/140‑1 (MQ=255)                                                              cGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGc                                                                      <  2:581931/140‑1 (MQ=255)                                                              cGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGc                                                                      <  1:504801/140‑1 (MQ=255)                                                              cGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGc                                                                      <  1:63970/140‑1 (MQ=255)                                                              cGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCAATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGc                                                                      <  2:590911/140‑1 (MQ=255)                                                                tCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCt                                                                    <  2:130695/140‑1 (MQ=255)                                                                   cGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCgg                                                                 <  2:629082/140‑1 (MQ=255)                                                                                cGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCg                                                    >  2:6966/1‑140 (MQ=255)                                                                                  ggCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGAc                                                  >  2:158135/1‑140 (MQ=255)                                                                                        gtgCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGa                                            >  1:251539/1‑140 (MQ=255)                                                                                           cGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATg                                         <  1:296056/140‑1 (MQ=255)                                                                                                gtTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCg                                    <  1:5275/140‑1 (MQ=255)                                                                                                 tttGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGt                                   >  2:1198672/1‑140 (MQ=255)                                                                                                 tttGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGt                                   <  2:765832/140‑1 (MQ=255)                                                                                                 tttGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGt                                   >  2:730956/1‑140 (MQ=255)                                                                                                  ttGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGtg                                  <  2:704956/140‑1 (MQ=255)                                                                                                  ttGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGtg                                  >  2:105453/1‑140 (MQ=255)                                                                                                   tGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGtga                                 >  2:883188/1‑140 (MQ=255)                                                                                                   tGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGtga                                 <  1:420466/140‑1 (MQ=255)                                                                                                          catcTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTaa                          <  1:158135/140‑1 (MQ=255)                                                                                                            tcTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAacg                        >  1:969834/1‑140 (MQ=255)                                                                                                            tcTGGCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAacg                        >  1:123751/1‑140 (MQ=255)                                                                                                               ggCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAacgacg                     >  1:15064/1‑140 (MQ=255)                                                                                                               ggCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAacgacg                     <  1:1204221/140‑1 (MQ=255)                                                                                                               ggCAGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAacgacg                     <  1:1198672/140‑1 (MQ=255)                                                                                                                  aGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGAcgcgc                  <  1:883188/140‑1 (MQ=255)                                                                                                                  aGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGAcgcgc                  <  1:336693/140‑1 (MQ=255)                                                                                                                  aGGCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGAcgcgc                  <  1:6966/140‑1 (MQ=255)                                                                                                                   ggCGCGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGAcgcgcg                 <  1:764480/140‑1 (MQ=255)                                                                                                                    gcgcGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACgcgcgc                >  2:715739/1‑140 (MQ=255)                                                                                                                    gcgcGGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACgcgcgc                <  1:939282/140‑1 (MQ=255)                                                                                                                      gcgGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCa              <  1:856260/140‑1 (MQ=255)                                                                                                                       cgGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAg             >  1:668788/1‑140 (MQ=255)                                                                                                                       cgGTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAg             <  2:984505/140‑1 (MQ=255)                                                                                                                        ggTGATCCCTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGt            <  2:668788/140‑1 (MQ=255)                                                                                                                                cTTCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTgg    >  1:525236/1‑140 (MQ=255)                                                                                                                                  tCGCCGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGAc  >  1:258354/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGAC  >  NC_000913/3616623‑3616891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑