Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,617,688	T→C	V225A (GTA→GCA)	nikD →	Ni(2(+)) ABC transporter ATP binding subunit NikD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,617,688	0	T	C	100.0%	171.0 / NA	50	V225A (GTA→GCA)	nikD	Ni(2(+)) ABC transporter ATP binding subunit NikD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/36);  total (14/36)

TCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGT  >  NC_000913/3617553‑3617823                                                                                                                                        |                                                                                                                                         tCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGaa                                                                                                                                      >  2:317648/1‑140 (MQ=255)  cGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGaaa                                                                                                                                     <  1:700565/140‑1 (MQ=255)    aTCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACg                                                                                                                                   <  1:1006172/140‑1 (MQ=255)    aTCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACg                                                                                                                                   <  1:85930/140‑1 (MQ=255)         ctgGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑tt                                                                                                                             >  1:812864/1‑140 (MQ=255)         ctgGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGGGATGTCTTACGGGAAGATTGTCGAACAGGGGGATGCAGAAAAGCTG‑tt                                                                                                                             >  2:1180596/1‑140 (MQ=255)          tgGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGGAAGATTGTCGAACAGGGGGATGCAGAAAAGCTG‑ttt                                                                                                                            >  2:691629/1‑140 (MQ=255)              aaGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACg                                                                                                                        <  2:1178067/140‑1 (MQ=255)               aGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGGTGTCTGACGGTAAGATTGTCGAACAGGGGGGAGGAGAAAAGCTG‑ttttaagc                                                                                                                       >  2:85930/1‑135 (MQ=255)               aGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGc                                                                                                                       >  2:728703/1‑140 (MQ=255)                  aTTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGcccc                                                                                                                    <  2:812864/140‑1 (MQ=255)                  aTTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGcccc                                                                                                                    <  1:13867/140‑1 (MQ=255)                   ttATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGccccc                                                                                                                   >  1:1035335/1‑140 (MQ=255)                   ttATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGccccc                                                                                                                   <  2:995803/140‑1 (MQ=255)                   ttATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGccccc                                                                                                                   >  1:1050577/1‑140 (MQ=255)                     aTGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCaa                                                                                                                 <  1:691629/140‑1 (MQ=255)                      tGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCaaa                                                                                                                >  1:510568/1‑140 (MQ=255)                       gCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAAc                                                                                                               <  1:96103/140‑1 (MQ=255)                           aaaCAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGGAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAAAATAc                                                                                                           >  1:1038264/1‑140 (MQ=255)                                  cgcCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGAcg                                                                                                    <  2:123682/140‑1 (MQ=255)                                    ccGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGAcgcg                                                                                                  <  2:817605/140‑1 (MQ=255)                                           tgctgcTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTg                                                                                           <  1:1180596/140‑1 (MQ=255)                                           tgctgcTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGGTATTTTTGACGGGAAGAATGTCGAAAAAGGCGGTGCAGGAAAACTG‑TTTAAAGCCCCCAAAAATAAAGAGACGGGCCGCCTg                                                                                           >  2:277909/1‑140 (MQ=255)                                             ctgctgGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTTGCGGGAAGATTGTCGAACACGGCGATGCAGAAACGCTG‑TTTTAAGCCCCCCAAAATAAAGAGACGCGCCGCCTggg                                                                                         >  2:572855/1‑139 (MQ=255)                                             ctgctgGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGt                                                                                         <  1:317648/140‑1 (MQ=255)                                                   gTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGGGATGTTTGACGGTAAGATTGTCGAACAGGGCGAGGCAGAAAAGCTG‑GTTTACGCCCCCAAACAAACAGTGACGCGCAGCCCGGTTTCCGc                                                                                   >  2:249993/1‑140 (MQ=255)                                                     agcccATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTc                                                                                 <  1:556737/138‑1 (MQ=255)                                                       cccATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCCAAAATACAGGGACGCGCAGCCTGGTTTTCGCCCAt                                                                               >  2:109729/1‑140 (MQ=255)                                                        ccATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCAtc                                                                              <  2:510568/140‑1 (MQ=255)                                                         cATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCAtct                                                                             <  1:728703/140‑1 (MQ=255)                                                          aTGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCAtctc                                                                            <  1:572855/140‑1 (MQ=255)                                                           tGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCg                                                                           <  2:1092871/140‑1 (MQ=255)                                                           tGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCAAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCg                                                                           <  2:633520/140‑1 (MQ=255)                                                           tGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGGAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCCAACATACAGGGACGCGCAGCCTGGGTTCCGCTCAtctcc                                                                           >  2:144416/1‑139 (MQ=255)                                                                  ggCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTa                                                                    <  1:912670/140‑1 (MQ=255)                                                                  ggCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTa                                                                    <  1:306179/140‑1 (MQ=255)                                                                  ggCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTa                                                                    <  1:464191/140‑1 (MQ=255)                                                                  ggCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGGAAGAATGTCGAACAAGGCGGTGCAGAAACGCTGTTTTAACCCCCCCAAA‑ATACAATGACGCGCAGCCCGGGTTCCGCTCATCTCGCCctccc                                                                    >  2:556737/1‑138 (MQ=255)                                                                     gTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGGAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAAAATAAAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACgg                                                                 >  1:10620/1‑140 (MQ=255)                                                                      ttGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGGCGGGAAGATTGTCGGACAGGGCGATGCAGAAAAGCTG‑TTTAACGCCCCCAAAAATACAGTGACGCGCAGCCTGGTTTTCGCTCATCTCGCCCCCTACggg                                                                >  2:99316/1‑139 (MQ=255)                                                                      ttGTGGCGCGTCTGGCGGATGACGTGGCGGGGATGTTTTACCGGAAGAATGTCGAACAGGGCGATGCAGAAAAGCTG‑TTTTACGCCCCCAAAAATACAAAGACGCGCAGCCTGGGTTTCCCTCATCTCCCCctcctcggg                                                                >  2:912670/1‑134 (MQ=255)                                                                       tgtgGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTa                                                               <  1:144416/140‑1 (MQ=255)                                                                        gtgGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGccc                                                                        >  1:136082/1‑130 (MQ=255)                                                                        gtgGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGccc                                                                        <  2:136082/130‑1 (MQ=255)                                                                        gtgGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTa                                                                    <  2:612006/134‑1 (MQ=255)                                                                        gtgGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTa                                                                    >  1:612006/1‑134 (MQ=255)                                                                               gTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTg                                                       <  1:705135/140‑1 (MQ=255)                                                                                   ggCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGcat                                                   <  2:103918/140‑1 (MQ=255)                                                                                                  ggTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTaa                                    <  2:1035335/140‑1 (MQ=255)                                                                                                  ggTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTaa                                    <  2:1050577/140‑1 (MQ=255)                                                                                                            gACGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGc                          >  1:1127712/1‑140 (MQ=255)                                                                                                             aCGGTAAGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGcc                         <  1:187118/140‑1 (MQ=255)                                                                                                                  aaGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTcc                    <  1:249993/140‑1 (MQ=255)                                                                                                                   aGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGcc                         >  1:1055915/1‑134 (MQ=255)                                                                                                                   aGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGcc                         <  2:1055915/134‑1 (MQ=255)                                                                                                                   aGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTccc                   >  1:1069591/1‑140 (MQ=255)                                                                                                                   aGATTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTccc                   <  2:959915/140‑1 (MQ=255)                                                                                                                     aTTGTCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCAt                 <  1:99316/140‑1 (MQ=255)                                                                                                                         tCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACt             >  2:1193931/1‑140 (MQ=255)                                                                                                                         tCGAACAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACt             >  2:1008830/1‑140 (MQ=255)                                                                                                                             aCAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATgc         >  1:246166/1‑140 (MQ=255)                                                                                                                              cAGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATgcg        >  2:490655/1‑140 (MQ=255)                                                                                                                               aGGGCGATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATgcgc       >  2:42789/1‑140 (MQ=255)                                                                                                                                    gATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGt  <  1:125364/140‑1 (MQ=255)                                                                                                                                    gATGCAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGt  <  2:1061504/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTG‑TTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGT  >  NC_000913/3617553‑3617823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑