Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	406,413	A→G	Q3Q (CAA→CAG)	aroL →	shikimate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	406,413	0	A	G	100.0%	334.7 / NA	92	Q3Q (CAA→CAG)	aroL	shikimate kinase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/43);  total (49/43)

TGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGA  >  NC_000913/406277‑406546                                                                                                                                          |                                                                                                                                      tgtATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCct                                                                                                                                    <  1:29258/140‑1 (MQ=255)   tATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCctct                                                                                                                                  >  1:572120/1‑140 (MQ=255)   tATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCctct                                                                                                                                  >  1:447102/1‑140 (MQ=255)   tATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCctct                                                                                                                                  >  1:663847/1‑140 (MQ=255)   tATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCctct                                                                                                                                  >  2:389250/1‑140 (MQ=255)        agaTTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTc                                                                                                                             >  1:1216354/1‑140 (MQ=255)          aTTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTg                                                                                                                           >  2:134432/1‑140 (MQ=255)            tttCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGAt                                                                                                                         <  2:254783/140‑1 (MQ=255)              tCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCg                                                                                                                       <  2:572120/140‑1 (MQ=255)                    tAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCg                                                                                                                       <  1:758442/134‑1 (MQ=255)                    tAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCg                                                                                                                       >  2:758442/1‑134 (MQ=255)                    tAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTc                                                                                                                 >  1:838780/1‑140 (MQ=255)                        tGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCgggg                                                                                                             <  2:1216354/140‑1 (MQ=255)                         ggAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGc                                                                                                            <  1:33335/140‑1 (MQ=255)                         ggAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGc                                                                                                            <  1:918783/140‑1 (MQ=255)                           aaTTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTGTGATCGGGCCTCGGGGCtg                                                                                                          <  2:814285/140‑1 (MQ=255)                           aaTTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCtg                                                                                                          <  2:838780/140‑1 (MQ=255)                                                   gTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGc                                                                                  >  2:1147833/1‑140 (MQ=255)                                                       tAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCtt                                                                              <  1:134432/140‑1 (MQ=255)                                                         gTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGc                                                                            >  2:413931/1‑140 (MQ=255)                                                            tGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGa                                                                         >  2:17342/1‑140 (MQ=255)                                                             gATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGAt                                                                        <  1:302076/140‑1 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGc                                                                            <  1:174064/136‑1 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGc                                                                            >  2:174064/1‑136 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGAtt                                                                       >  1:367422/1‑140 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGAtt                                                                       >  2:194941/1‑140 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGAtt                                                                       >  1:471436/1‑140 (MQ=255)                                                              aTGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGAtt                                                                       >  1:549735/1‑140 (MQ=255)                                                                ggTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCg                                                                     >  1:579296/1‑140 (MQ=255)                                                                  tATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCt                                                                   <  1:462039/140‑1 (MQ=255)                                                                   aTGATCGCTATTCTCATGACACCGGCTTTCGTCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCtt                                                                  >  2:631735/1‑140 (MQ=255)                                                                   aTGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCtt                                                                  <  1:435863/140‑1 (MQ=255)                                                                         gCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGt                                                            >  1:999576/1‑140 (MQ=255)                                                                          cTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTc                                                           >  1:147672/1‑140 (MQ=255)                                                                          cTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTACT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTAGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTc                                                           >  2:602971/1‑140 (MQ=255)                                                                           tATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCg                                                          >  2:273673/1‑140 (MQ=255)                                                                           tATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCg                                                          >  2:1139334/1‑140 (MQ=255)                                                                           tATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCg                                                          >  2:735278/1‑140 (MQ=255)                                                                           tATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCg                                                          >  2:1060613/1‑140 (MQ=255)                                                                            aTTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCgg                                                         <  2:447102/140‑1 (MQ=255)                                                                            aTTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCgg                                                         <  1:1171514/140‑1 (MQ=255)                                                                            aTTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCgg                                                         <  2:147672/140‑1 (MQ=255)                                                                            aTTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCgg                                                         >  1:908047/1‑140 (MQ=255)                                                                             ttCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGt                                                        <  1:389250/140‑1 (MQ=255)                                                                             ttCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGt                                                        <  1:273673/140‑1 (MQ=255)                                                                                tcATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTg                                                     >  1:270716/1‑140 (MQ=255)                                                                                 cATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGt                                                    >  1:1020056/1‑140 (MQ=255)                                                                                 cATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGt                                                    <  1:1147833/140‑1 (MQ=255)                                                                                  aTGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTc                                                   >  2:275458/1‑140 (MQ=255)                                                                                   tGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCg                                                  >  1:740515/1‑140 (MQ=255)                                                                                     acacCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATGGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCACTTAACCGTCGGTTTGTCGAt                                                >  2:1105771/1‑140 (MQ=255)                                                                                     acacCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGAt                                                <  2:17632/140‑1 (MQ=255)                                                                                      cacCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATa                                               <  1:1105771/140‑1 (MQ=255)                                                                                      cacCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATa                                               <  1:614032/140‑1 (MQ=255)                                                                                       acCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGt                                                        <  2:1013048/130‑1 (MQ=255)                                                                                       acCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGt                                                        >  1:1013048/1‑130 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTc                                                           >  2:441749/1‑125 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTc                                                           <  1:441749/125‑1 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGt                                                    >  2:1039756/1‑132 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGt                                                    <  1:1039756/132‑1 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATAcc                                             >  1:61567/1‑139 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATAcc                                             <  2:61567/139‑1 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCg                                            >  1:1017549/1‑140 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCg                                            >  2:304626/1‑140 (MQ=255)                                                                                         cGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCg                                            >  2:125465/1‑140 (MQ=255)                                                                                          ggCTTTCGCCGCATTGCGACCTACT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTAGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGa                                           <  1:602971/140‑1 (MQ=255)                                                                                           gCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGAt                                          <  1:17342/140‑1 (MQ=255)                                                                                              ttCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAg                                       <  2:613697/140‑1 (MQ=255)                                                                                              ttCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAg                                       <  2:1020056/140‑1 (MQ=255)                                                                                                 gccgcATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCg                                                  <  2:582015/126‑1 (MQ=255)                                                                                                 gccgcATTGCGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCg                                                  >  1:582015/1‑126 (MQ=255)                                                                                                          cGACCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATca                           <  2:1017549/140‑1 (MQ=255)                                                                                                            aCCTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATcaca                         >  2:516245/1‑140 (MQ=255)                                                                                                             ccTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAg                        <  1:125465/140‑1 (MQ=255)                                                                                                             ccTATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAg                        >  2:376558/1‑140 (MQ=255)                                                                                                               tATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCt                      <  1:735278/140‑1 (MQ=255)                                                                                                               tATT‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCt                      <  1:376558/140‑1 (MQ=255)                                                                                                                  t‑GGGGAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCaa                   >  1:1065983/1‑140 (MQ=255)                                                                                                                      ggAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAatat                <  2:579296/140‑1 (MQ=255)                                                                                                                      ggAAAACCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTC‑GGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATg               >  2:537624/1‑140 (MQ=255)                                                                                                                        aaaaCCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa              <  2:549735/140‑1 (MQ=255)                                                                                                                        aaaaCCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa              >  1:122748/1‑140 (MQ=255)                                                                                                                          aaCCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg            <  2:122748/140‑1 (MQ=255)                                                                                                                          aaCCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg            >  1:1206972/1‑140 (MQ=255)                                                                                                                          aaCCCACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg            >  2:266727/1‑140 (MQ=255)                                                                                                                            cccACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa              >  1:782733/1‑136 (MQ=255)                                                                                                                            cccACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa              <  2:782733/136‑1 (MQ=255)                                                                                                                            cccACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGt          >  1:530466/1‑140 (MQ=255)                                                                                                                            cccACGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGt          >  1:1080326/1‑140 (MQ=255)                                                                                                                                cGATGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTcgcg      <  2:801604/140‑1 (MQ=255)                                                                                                                                  aTGACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGga    <  2:367422/140‑1 (MQ=255)                                                                                                                                    gACACAGCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGgaga  <  2:740515/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGACCTATT‑GGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGA  >  NC_000913/406277‑406546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑