| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,743,478 | T→C | V249A (GTC→GCC) | yiaK → | 2,3‑diketo‑L‑gulonate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,743,478 | 0 | T | C | 96.4% | 182.6 / ‑3.0 | 56 | V249A (GTC→GCC) | yiaK | 2,3‑diketo‑L‑gulonate reductase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (27/27); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATTTGACCAAAGAACCTGGCGTTATCGAGAAGAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGTCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTG > NC_000913/3743343‑3743612 | aTTTGACCAAAGAACCTGGCGTTATCGAGAAGAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCAcc < 1:868396/140‑1 (MQ=255) tGACCAAAGAACCTGGCGTTATCGAGAAGAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAg < 1:307488/140‑1 (MQ=255) aGACCCTGGCGTTATCGAGAAGAATCGCTGCATTTTGCCGATGGGCTCCTGTAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTACCGACGGCGCATCCGTTGCCGAAGCCCCCCAGGacaaca < 2:250157/140‑1 (MQ=255) cTGGCGTTATCGAGAAGAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGcgac > 2:358432/1‑140 (MQ=255) agaagaATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCAtt > 1:414648/1‑140 (MQ=255) tCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACaaa > 2:112863/1‑140 (MQ=255) ccgcATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAAttt > 2:358414/1‑140 (MQ=255) cgcATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAAtttt > 2:625919/1‑140 (MQ=255) aTTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTAt < 1:388179/140‑1 (MQ=255) ttttGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCAACCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTAtt > 2:206505/1‑140 (MQ=255) tGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGcc > 2:2352/1‑140 (MQ=255) gCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCa < 1:206505/140‑1 (MQ=255) aTGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGa > 1:999876/1‑140 (MQ=255) tGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGaa < 1:1192098/140‑1 (MQ=255) cTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTgg > 1:612962/1‑140 (MQ=255) cTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTACCATTGAAGTGGACa < 1:358432/140‑1 (MQ=255) tGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACg < 2:1084609/140‑1 (MQ=255) tGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACg < 1:112863/140‑1 (MQ=255) tGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTccc < 1:1042878/140‑1 (MQ=255) tGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTccc > 1:779683/1‑140 (MQ=255) gTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGt < 2:1215049/136‑1 (MQ=255) gTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGt < 2:287154/136‑1 (MQ=255) gTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGt > 1:1215049/1‑136 (MQ=255) gTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGt > 1:287154/1‑136 (MQ=255) gTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCa < 1:973699/140‑1 (MQ=255) tCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCAc > 1:969535/1‑140 (MQ=255) gtgCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGa < 1:625919/140‑1 (MQ=255) ggATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCa < 1:1084003/140‑1 (MQ=255) ggATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCa < 2:393569/140‑1 (MQ=255) tatGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGc > 1:1118888/1‑140 (MQ=255) aTCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCa < 2:1118888/140‑1 (MQ=255) tctcTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACAGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGGGATGCCAAGCTGCaa < 2:168736/137‑1 (MQ=255) tCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCaa > 2:486897/1‑140 (MQ=255) tCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCaa < 1:2352/140‑1 (MQ=255) tCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCaa > 1:121536/1‑140 (MQ=255) tCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCaa < 2:999876/140‑1 (MQ=255) cTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGc > 1:164309/1‑140 (MQ=255) tACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGGGGACAAGCTTATCAACGGTCCCACCCGCGATGCCAAGCTGCAACGca > 2:362901/1‑140 (MQ=255) aCTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGTCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGcat > 1:855210/1‑140 (MQ=255) aCTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGcat < 2:121536/140‑1 (MQ=255) ctctCCTTTCCGACGGCGCATCCGTTGCCGAAGTCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGcatc < 2:855210/140‑1 (MQ=255) tcCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATg > 1:85276/1‑140 (MQ=255) tcCTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATg < 2:923514/140‑1 (MQ=255) cTTTCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGa > 2:886419/1‑140 (MQ=255) tCCGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTa > 1:229558/1‑140 (MQ=255) ccGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTAc < 1:928350/140‑1 (MQ=255) cGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATgg < 1:764835/134‑1 (MQ=255) cGACGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATgg > 2:764835/1‑134 (MQ=255) cGGCGCATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTa < 2:969535/140‑1 (MQ=255) gcgcATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGcat < 2:1093836/124‑1 (MQ=255) gcgcATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGcat > 1:1093836/1‑124 (MQ=255) gcgcATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATgg < 2:444799/129‑1 (MQ=255) gcgcATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATgg > 1:444799/1‑129 (MQ=255) cATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGt > 1:1196188/1‑140 (MQ=255) cATCCGTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGt > 1:1065450/1‑140 (MQ=255) gTTGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGa < 2:821966/140‑1 (MQ=255) ttGCCGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAg > 1:1212478/1‑140 (MQ=255) cGAAGCCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTg < 1:886419/140‑1 (MQ=255) | ATTTGACCAAAGAACCTGGCGTTATCGAGAAGAATCGCCGCATTTTGCCGATGGGCTACTGGAAAGGTTCTGGCATGTCGATTGTGCTGGATATGATCGCTACTCTCCTTTCCGACGGCGCATCCGTTGCCGAAGTCACCCAGGACAACAGCGACGAATACGGCATTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTG > NC_000913/3743343‑3743612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |